| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Outline of Final Research Achievements |
A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) system based on a voltammetric reduction of quinone was developed to perform the metabolomics of fecal organic acids in intestinal flora. The present HPLC-ECD system performed the determination of fecal organic acids (lactic, acetic, propionic, butyric, isovaleric, and valeric acids) in rats. Moreover, a change of fecal organic acids in rats after ingestion of an antimicrobial agent and a Kampo medicine was monitored by the present HPLC-ECD system. It was shown that the present HPLC-ECD system was useful to examine the effect of foods and medicines on bioactivities of intestinal flora.
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