Development of an analytical method for metabolomics of fecal organic acid in intestinal flora
| Project/Area Number |
26410161
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
KOTANI Akira 東京薬科大学, 薬学部, 准教授 (40318184)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 有機酸 / 電気分析化学 / 液体クロマトグラフィー / 糞便 / メタボロミクス |
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| Outline of Final Research Achievements |
A high-performance liquid chromatography with electrochemical detection (HPLC-ECD) system based on a voltammetric reduction of quinone was developed to perform the metabolomics of fecal organic acids in intestinal flora. The present HPLC-ECD system performed the determination of fecal organic acids (lactic, acetic, propionic, butyric, isovaleric, and valeric acids) in rats. Moreover, a change of fecal organic acids in rats after ingestion of an antimicrobial agent and a Kampo medicine was monitored by the present HPLC-ECD system. It was shown that the present HPLC-ECD system was useful to examine the effect of foods and medicines on bioactivities of intestinal flora.
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Report
(4 results)
Research Products
(9 results)
