Inovation in fluorous chemistry focusing on selective analysis of peptides and proteins
| Project/Area Number |
26410166
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Masatoshi 福岡大学, 薬学部, 教授 (50117280)
HAYAMA Tadashi 福岡大学, 薬学部, 准教授 (90549693)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | フルオラス化学 / 前処理 / ペプチド / タンパク質 / 誘導体化 / アミノ基 / リン酸基 / ペプチド分析 / タンパク質分析 / パーフルオロアルキル / LC/MS/MS / パーフルオラス化合物 / フルオラスカラム / 分析化学 / フルオラスケミストリー / 分離分析 / 液体クロマトグラフィー / 金属キレートアフィニティー / 溶媒・固相抽出 |
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| Outline of Final Research Achievements |
Highly selective and effective purification methods for peptides and proteins have been developed. One is for polyamino-containing peptides. The amino moieties of peptides are perfluoroalkylated with amine-reactive fluorous reagent, then the derivatives are highly selectively extracted to a fluorous solvent, followed by LC/MS/MS analysis. The other is for phosphorylated peptides. The Fe(III) immobilized fluorous reagent is designed and synthesized. The reagent reacts with phosphorylated peptides to form their fluorous chelates, those are extracted selectively into a fluorous solvent. The former is applied to the determination of brain peptides in rat brain samples, and the latter to the assay for protein kinase activity.
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Report
(5 results)
Research Products
(41 results)
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[Journal Article] Assessment of the Efficacy of Anticancer Drugs by Amino Acid Metabolomics Using Fluorescence Derivatization-HPLC2014
Author(s)
Ryoko Tomita, Kenichiro Todoroki, Kazuyuki Machida, Sho Nishida, Hiroshi Maruoka, Hideyuki Yoshida, Toshihiro Fujioka, Manabu Nakashima, Masatoshi Yamaguchi, Hitoshi Nohta
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Journal Title
Analytical Sciences
Volume: 30
Issue: 7
Pages: 751-758
DOI
NAID
ISSN
0910-6340, 1348-2246
Related Report
Peer Reviewed / Open Access
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