| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
We proposed a new analytical method to detect the methylation state of cytosine in DNA utilizing the rotation of mismatched DNA bases, and evaluated properties and fabricated a micro-sensing device. Conventionally, although the total amount of methylcytosine can be measured with antibody, the utility value is poor due to unknown position information in the DNA sequence. We challenged sequence-selective antibody recognition utilizing outward turned mismatched bases and rapid measurement by micro-sensing device fabrication. We have developed a novel SPR chip (epigenetic chip) to realize methylation assessment of oligo- and genomic- DNAs in a small SPR instrument.
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