Elucidation of electronic control of hemoprotein function and its application to construct artificial blood substitute
Project/Area Number |
26410172
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bio-related chemistry
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Research Institution | University of Tsukuba |
Principal Investigator |
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Research Collaborator |
SHIBATA Tomokazu
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | ヘムタンパク質 / ヘム電子構造 / 配位子結合反応 / 機能調節 / 人工酸素運搬体 |
Outline of Final Research Achievements |
We analyzed the functional properties of myoglobin and its mutant proteins reconstituted with heme cofactors possessing a heme Fe atom with a variety of electron densities. The study revealed that the oxygen (O2) affinities of the proteins are regulated by the electron density of the heme Fe atom (R(Fe)) in such a manner that the O2 affinity of the protein decreases, due to an increase in the O2 dissociation rate constant, with a decrease in the R(Fe). On the other hand, the carbon monoxide (CO) affinities of the proteins were almost independent of the R(Fe). As a result, the regulation of the O2/CO discrimination in the proteins is controlled by the R(Fe). Thus the electronic tuning of the intrinsic heme Fe reactivity through the R(Fe) plays a vital role in the regulation of the protein function, as the heme environment furnished by the nearby amino acid residues does. These findings pave the way to construct artificial blood substitute.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Effects of Heme Electronic Structure and Distal Polar Interaction on Functional and Vibrational Properties of Myoglobin2016
Author(s)
Y. Kanai, R. Nishimura, K. Nishiyama, T. Shibata, S. Yanagisawa, T. Ogura, T. Matsuo, S. Hirota, S. Neya, A. Suzuki, Y. Yamamoto
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Journal Title
Inorg. Chem.
Volume: 55
Issue: 4
Pages: 1613-1622
DOI
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Peer Reviewed / Open Access
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[Journal Article] Electronic control of ligand-binding preference of a myoglobin mutant2014
Author(s)
R. Nishimura, D. Matsumoto, T. Shibata, S. Yanagisawa, T. Ogura, H. Tai, T. Matsuo, S. Hirota, S. Neya, A. Suzuki, Y. Yamamoto
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Journal Title
Inorganic Chemistry
Volume: 53
Issue: 17
Pages: 9156-9165
DOI
Related Report
Peer Reviewed
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[Presentation] ヘムの系統的化学修飾および遠位アミノ酸置換がミオグロビンの機能に与える影響2017
Author(s)
渡邉美帆, 金井佑生, 西村龍, 柴田友和, 松尾貴史, 廣田俊, 柳澤幸子, 小倉尚志, 鈴木秋弘, 根矢三郎, 山本泰彦
Organizer
日本化学会 第97春季年会(2017)
Place of Presentation
慶応義塾大学日吉キャンパス(神奈川県横浜市)
Year and Date
2017-03-16
Related Report
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[Presentation] ヘムと四重鎖DNAの複合体の構造解析2017
Author(s)
越智健太郎, 山本泰彦, 柴田友和, 萩原正規, 逸見光
Organizer
日本化学会 第97春季年会(2017)
Place of Presentation
慶応義塾大学日吉キャンパス(神奈川県横浜市)
Year and Date
2017-03-16
Related Report
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[Presentation] Characterization of Complexes between Hemes and Parallel G-Quadruplex DNAs2016
Author(s)
Y. Yamamoto, T. Shibata, Y. Katahira, Y. Nakayama, H. Tai, T. Matsui, K. Morihashi, A. Watanabe, T. Nakao, S. Yanagisawa, T. Ogura, A. Suzuki, S. Neya
Organizer
8th Asian Bioligical Inorganic Chemistry Conference(AsBIC 8)
Place of Presentation
The University of Auckland(Auckland, New Zealand)
Year and Date
2016-12-04
Related Report
Int'l Joint Research
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[Presentation] Electronic Control of Myoglobin Function2015
Author(s)
Y. Yamamoto, R. Nishimura, Y. Kanai, T. Shibata, S. Yanagisawa, T. Ogura, H. Tai, T. Matsuo, S. Hirota, S. Neya, and A. Suzuki
Organizer
2015 環太平洋国際化学会議
Place of Presentation
Honolulu, USA
Year and Date
2015-12-15
Related Report
Int'l Joint Research / Invited
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[Presentation] Heme-DNA Complexes2015
Author(s)
Masashi Kinoshita, Tomokazu Shibata, Hikaru Hemmi, and Yasuhiko Yamamoto
Organizer
5th International Meeting on Quadruplex Nucleic Acids
Place of Presentation
Bordeaux (France)
Year and Date
2015-05-26 – 2015-05-28
Related Report
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[Presentation] Heme-DNA Complexes2015
Author(s)
M. Kinoshita, T. Shibata. H. Hemmi, and Y Yamamoto
Organizer
5th International Meeting on Quadruplex Nucleic Acids: G4thering in Bordeaux
Place of Presentation
Bordeaux, France
Year and Date
2015-05-26
Related Report
Int'l Joint Research
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[Presentation] Electronic Regulation of Ligand-Binding Preference of the L29F Mutant of Myoglobin2014
Author(s)
T. Shibata, R. Nishimura, D. Matsumoto, S. Yanagisawa, T. Ogura, H. Tai, T. Matsuo, S. Hirota, S. Neya, A. Suzuki, and Y. Yamamoto
Organizer
Michinoku International Symposium on Porphyrins, Phthalocyanines and Functional Molecules
Place of Presentation
宮城蔵王ロイヤルホテル(宮城県刈田郡蔵王町)
Year and Date
2014-10-14 – 2014-10-16
Related Report
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[Presentation] 一酸化炭素よりも酸素に対して高い親和性を示すミオグロビンの創製2014
Author(s)
西村 龍, 松本 大地, 柴田 友和, 柳澤 幸子, 小倉 尚志, 太 虎林, 松尾 貴史, 廣田 俊, 根矢 三郎, 鈴木 秋弘, 山本 泰彦
Organizer
第52回日本生物物理学会年会(BSJ2014)
Place of Presentation
札幌コンベンションセンター(北海道札幌市)
Year and Date
2014-09-13 – 2014-09-15
Related Report
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[Presentation] 酸素結合型ミオグロビンにおける量子化学的共鳴を通した酸素親和性および自動酸化反応速度の調節2014
Author(s)
西村龍, 西山康太郎, 松本大地, 柴田友和, 石上泉, 小倉尚志, 太虎林, 長尾聡, 松尾貴史, 廣田俊, 荘司長三, 渡辺芳人, 今井清博, 根矢三郎, 鈴木秋弘, 山本泰彦
Organizer
第41回生体分子科学討論会
Place of Presentation
九州大学西新プラザ(福岡県福岡市)
Year and Date
2014-06-06 – 2014-06-07
Related Report
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