| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Aminoglycoside antibiotics contain a unique aminocyclitol that is decorated with various aminosugars leading to a wide variety of structures including kanamycin and neomycin. A common biosynthetic scheme of kanamycin and neomycin has been elucidated, when this project started. Thus, unique enzymatic modifications of common biosynthetic intermediates were speculated to be involved in the individual biosynthetic machinery. Radical S-adenosyl-L-methionine (SAM) enzymes that are often encoded in the biosynthetic gene clusters are supposed to be responsible for such unique reaction, because radical SAM enzyme generates 5′-deoxyadenosyl radical that can trigger various radical reactions in living system. In current research program, I focused on the functional analysis of radical SAM epimerase NeoN in neomycin B biosynthesis and radical SAM dehydratase AprD4 in apramycin biosynthesis. Consequently, we could clarify the functions of NeoN and AprD4 and propose plausible reaction mechanisms.
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