Development of life-survey methods using SERS probes
Project/Area Number |
26410179
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bio-related chemistry
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Research Institution | Kyoto Institute of Technology |
Principal Investigator |
KOBORI AKIO 京都工芸繊維大学, 分子化学系, 准教授 (00397605)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | SERS / ラマン散乱 / DNA / RNA / miRNA / エピジェネティクス |
Outline of Final Research Achievements |
It is reported that concentration of particular RNAs in body fluids is associated with the onset of the diseases. Therefore the RNAs in blood serum, urine, and saliva are anticipated to be used as biomarkers for the diagnosis of severe diseases. Although reverse transcription quantitative PCR is generally used to quantify RNAs, the PCR has been poorly utilized as a diagnostic method due to a complicated operation for amplification of nucleic acids. Until now, we have developed a highly sensitive and simple nucleic acid detection system that does not require the amplification step of nucleic acids. In this study, we developed a chemical tag with a Raman signal in a region that does not appear any signals of the biological component in the Raman spectrum and constructed a sandwich assay system using a SERS positive tag.
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Report
(4 results)
Research Products
(32 results)
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[Journal Article] Development of Novel Antisense Oligonucleotides for the Functional Regulation2015
Author(s)
Ariyoshi, J., Momokawa, D., Eimori, N., Kobori, A., Murakami A., Yamayoshi, A.
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Journal Title
Bioconjugate Chemistry
Volume: 26
Issue: 12
Pages: 2454-2460
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Development of a novel drug delivery system for targeting microRNA2016
Author(s)
○KISHIMOTO, Yusuke, TAMURA, Rie, MURAMATSU, Chie, KOBORI, Akio, ASHIHARA, Eishi, MURAKAMI, Akira YAMAYOSHI, Asako
Organizer
日本化学会第96春季年会
Place of Presentation
同志社大学 京田辺キャンパス
Year and Date
2016-03-24
Related Report
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[Presentation] Development of a novel system for evaluation of binding affinities between RISC and antisense oligonucleotides2016
Author(s)
○KONISHI, Ryo, ARIYOSHI, Jumpei, MATSUYAMA, Yohei, NAKAMURA, Hiromi1, KOBORI, Akio, MURAKAMI, Akira YAMAYOSHI, Asako
Organizer
日本化学会第96春季年会
Place of Presentation
同志社大学 京田辺キャンパス
Year and Date
2016-03-24
Related Report
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