| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Outline of Final Research Achievements |
It is reported that concentration of particular RNAs in body fluids is associated with the onset of the diseases. Therefore the RNAs in blood serum, urine, and saliva are anticipated to be used as biomarkers for the diagnosis of severe diseases. Although reverse transcription quantitative PCR is generally used to quantify RNAs, the PCR has been poorly utilized as a diagnostic method due to a complicated operation for amplification of nucleic acids. Until now, we have developed a highly sensitive and simple nucleic acid detection system that does not require the amplification step of nucleic acids. In this study, we developed a chemical tag with a Raman signal in a region that does not appear any signals of the biological component in the Raman spectrum and constructed a sandwich assay system using a SERS positive tag.
|
