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Sialyllactose - modified 3-way junction DNA as a inhibitor of influenza hemagglutinin

Research Project

Project/Area Number 26410180
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionKobe University

Principal Investigator

Ebara Yasuhito  神戸大学, 人間発達環境学研究科, 准教授 (40251657)

Co-Investigator(Kenkyū-buntansha) 中村 晴信  神戸大学, 人間発達環境学研究科, 教授 (10322140)
開發 邦宏  大阪大学, 産業科学研究所, 特任准教授(常勤) (70419464)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsインフルエンザウイルス / 3-way junction DNA / シアリルラクトース / ヘマグルチニン / 検出 / DNAポリメラーゼ / インフルエンザ / DNA / シアリダーゼ / three way junction
Outline of Final Research Achievements

Influenza is one of the most infectious diseases in the world. On the virus, there are hemagglutinin(HA) protein that recognizes sialic acid (SA) on a cell surface and plays an important role in the first step of infection. So the compounds that bind strongly to HA would be useful for various influenza virus detection as the corresponding antibodies because the amino acid sequence of HA’s SA binding sites are highly conserved if virus mutations occur.
In this study, Sialyllactose (SL) -modified 3-way junction DNAs were synthesized using SL-modified dUTP and DNA polymerase. One of the 3WJ DNA showed 80,000-fold higher binding affinity for influenza virus A/Puerto Rico/08/34 (H1N1) compared to the 2,3-SL. This resultindicated that the glycocluster effect and optimal spatial arrangement of the 3WJ DNA improved the weak interactions between a sialic acid and its binding site on HA. This 3WJ DNA compound has possible application as an inhibitor of influenza infection and for virus sensing.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2016 2015 2014

All Presentation (13 results) (of which Int'l Joint Research: 4 results) Book (1 results)

  • [Presentation] Sialyllactose-modified 3-way junction DNA that have Influenza virus binding ability2016

    • Author(s)
      山部 美幸、開發 邦宏、江原 靖人
    • Organizer
      Japan-Taiwan Medical Spectroscopy International Symposium
    • Place of Presentation
      淡路夢舞台国際会議場(兵庫県淡路市)
    • Year and Date
      2016-12-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] インフルエンザウイルスと結合するシアリルラクトース修飾 DNA2016

    • Author(s)
      山部 美幸、開發 邦宏、江原 靖人
    • Organizer
      第65回高分子学会年次大会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] Expanding glycoconjugate library on nucleic acids and its enhanced affinity to lectins2016

    • Author(s)
      江原 靖人、松田 美加、山部 美幸
    • Organizer
      第65回高分子学会年次大会
    • Place of Presentation
      神戸国際会議場(兵庫県神戸市)
    • Year and Date
      2016-05-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] シアル酸修飾オリゴヌクレオチド担持ナノ粒子を用いたインフルエンザウイルスの検出2016

    • Author(s)
      赤松大地、山部美幸、江原靖人
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府・京田辺市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Presentation] あらゆるインフルエンザウイルスと結合するシアル酸修飾3-way junction DNAの合成2016

    • Author(s)
      山部美幸、開發邦宏、江原靖人
    • Organizer
      日本化学会第96春季年会
    • Place of Presentation
      同志社大学(京都府・京田辺市)
    • Year and Date
      2016-03-24
    • Related Report
      2015 Research-status Report
  • [Presentation] Sialyllactose-modified 3-way junction DNA as a inhibitor of influenza hemagglutinin2015

    • Author(s)
      山部美幸、赤松大地、開發邦宏、加藤修雄、江原靖人
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Detection of influenza viruses by using oligonucleotide-based sialic acid-modified nanoparticle2015

    • Author(s)
      赤松大地、山部美幸、江原靖人
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Expanding glycoconjugate library on DNAs and its enhanced affinity to lectins2015

    • Author(s)
      江原靖人、乃村勇輝、松田美加、山部美幸、赤松大地
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2015-12-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] あらゆるインフルエンザウイルスを捕捉するシアル酸修飾3-way junction型DNA2015

    • Author(s)
      山部美幸、乃村勇輝、松田美加、赤松大地、浅井佑介、江原靖人
    • Organizer
      第61回高分子研究発表会
    • Place of Presentation
      兵庫県民会館(兵庫県・神戸市)
    • Year and Date
      2015-07-17
    • Related Report
      2015 Research-status Report
  • [Presentation] あらゆるインフルエンザウイルスを捕捉する シアル酸修飾 3-way junction 構造 DNA2015

    • Author(s)
      山部美幸、赤松大地、開發邦宏、江原靖人
    • Organizer
      第64回高分子学会年次大会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2015-05-27
    • Related Report
      2015 Research-status Report
  • [Presentation] シアリルラクトース修飾3-way junction型DNAを用いたインフルエンザウイルスの検出2015

    • Author(s)
      山部美幸
    • Organizer
      日本化学会第95春季年会
    • Place of Presentation
      日本大学船橋キャンパス
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] あらゆるインフルエンザウイルスと結合する糖鎖修飾 three-way junction DNA2014

    • Author(s)
      山部美幸
    • Organizer
      第60回高分子研究発表会(神戸)
    • Place of Presentation
      兵庫県民会館
    • Year and Date
      2014-07-25
    • Related Report
      2014 Research-status Report
  • [Presentation] あらゆるインフルエンザウイルスと結合する シアリルラクトース修飾 3-way junction型 DNA2014

    • Author(s)
      江原靖人
    • Organizer
      第63回高分子学会年次大会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2014-05-28 – 2014-05-30
    • Related Report
      2014 Research-status Report
  • [Book] 現代化学 2月号2015

    • Author(s)
      江原靖人
    • Total Pages
      6
    • Publisher
      東京化学同人
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2019-03-29  

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