Introduction of lactam with useful functions into proteins at specific lysine residue by reactions with artificial nucleic acids
| Project/Area Number |
26410181
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kyushu University |
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Principal Investigator |
Aso Mariko 九州大学, 薬学研究院, 准教授 (30201891)
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| Co-Investigator(Renkei-kenkyūsha) |
KATAYAMA Tsutomu 九州大学, 大学院薬学研究院, 教授 (70264059)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 核酸 / 蛋白質 / リジン / ラクタム / 蛍光 / たんぱく質 / コンジュゲート / 修飾 / DNA / 核酸関連化学 / 蛋白質修飾 |
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| Outline of Final Research Achievements |
We developed oligodeoxynucleotides (ODNs) containing 1,4-dicarbonyl groups to modify specific lysine residue in DNA-interacting proteins and provide information on position and function of the lysine residue in question. Reductive amination of ODNs containing 1,4-dicarbonyl groups in ribose moiety proceeded with a proximal lysine residue in protein-ODN complex and cross-link products were obtained efficiently. ODNs containing 1,4-dicarbonyl groups on substituted benzene ring modified a proximal lysine residue to produce stable fluorescent compound and turn-on fluorescent protein modification at a specific lysine residue was achieved.
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Report
(4 results)
Research Products
(18 results)
