| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We developed oligodeoxynucleotides (ODNs) containing 1,4-dicarbonyl groups to modify specific lysine residue in DNA-interacting proteins and provide information on position and function of the lysine residue in question. Reductive amination of ODNs containing 1,4-dicarbonyl groups in ribose moiety proceeded with a proximal lysine residue in protein-ODN complex and cross-link products were obtained efficiently. ODNs containing 1,4-dicarbonyl groups on substituted benzene ring modified a proximal lysine residue to produce stable fluorescent compound and turn-on fluorescent protein modification at a specific lysine residue was achieved.
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