Tailor-made artificial enzymes (catalytic antibodies) by the development of holo-abzymes
Project/Area Number |
26410184
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Bio-related chemistry
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Research Institution | Osaka Prefecture University |
Principal Investigator |
Tsumuraya Takeshi 大阪府立大学, 理学(系)研究科(研究院), 准教授 (00372855)
|
Co-Investigator(Kenkyū-buntansha) |
藤井 郁雄 大阪府立大学, 理学(系)研究科(研究院), 教授 (70189984)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | 酵素 / モノクローナル抗体 / 抗体酵素 / 免疫学 / タンパク質 / 分子認識 / 生体分子 / 人工酵素 / 抗体工学 / 抗体化学 / 抗原 / ハイブリドーマ / 遷移状態アナログ / X線結晶構造解析 |
Outline of Final Research Achievements |
In this work we have developed holo-abzyme in which the antigen-combining site can bind the artificial cofactors which have functional groups for the catalysis. The holo-abzyme catalyzes various types of chemical transformations by simply exchanging the artificial cofactors. We also succeeded in the X-ray crystal analysis of holo-abzyme 27C1, showing the molecular basis of the catalysis by 27C1.
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Report
(4 results)
Research Products
(30 results)
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[Presentation] 抗体酵素6D9の触媒活性の向上2015
Author(s)
吉村美穂,武田祐輔,宮本尚樹,円谷 健,藤井郁雄
Organizer
第9回バイオ関連化学シンポジウム
Place of Presentation
熊本大学黒髪キャンパス(熊本県熊本市)
Year and Date
2015-09-10
Related Report
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