| Project/Area Number |
26410185
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
丹羽 一樹 国立研究開発法人産業技術総合研究所, その他部局等, 研究員 (30443211)
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| Co-Investigator(Renkei-kenkyūsha) |
NEGORO Seiji 兵庫県立大学, 大学院工学研究科, 教授 (90156159)
TAKEO Masahiro 兵庫県立大学, 大学院工学研究科, 准教授 (40236443)
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| Research Collaborator |
NAKAJIMA Yoshihiro 国立研究開発法人産業技術総合研究所, 健康工学研究部門, 研究グループ長
SHIRAKAWA Daiki 兵庫県立大学, 大学院工学研究科, 学生
OKUDA Masatoshi 兵庫県立大学, 大学院工学研究科, 学生
MAEDA Juri 鹿児島大学, 大学院理工学研究科, 学生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | バイオテクノロジー / ホタル / 生物発光 / 生合成経路 / トランスクリプトーム解析 |
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| Outline of Final Research Achievements |
In this project, we tried to establish a chiral free bioluminescence system inspired by the biosynthetic pathway of firefly D-luciferin. In fireflies, L-luciferin is the biosynthetic precursor of D-luciferin, which is produced from the L-form undergoing deracemization. This deracemization consists of three successive reactions: L-enantioselective thioesterification by luciferase, in situ epimerization, and hydrolysis by thioesterase. At first, a deracemizative luminescence system using a combination of firefly luciferase and fatty acyl-CoA thioesterase from bacteria was constructed. We could confirm that this firefly luciferase-thioesterase combination system worked well and can improve bioanalysis applications using the firefly bioluminescence reaction by efficient deracemization of D-luciferin. We also performed a transcriptome analysis toward adult firefly.
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