Effect of DNA secondary structures and chemical environments on the regulation of rate of gene replication
| Project/Area Number |
26410188
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Konan University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | DNA / 複製 / グアニン四重鎖 / 分子クラウディング / i-motif |
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| Outline of Final Research Achievements |
In this study, we observed DNA repliation reaction in time and quantitatively investigated the replication rate depending on the stabiity of the template DNA and surronding chemical environments. As a result of analysis of the collerations between the stability of the template DNA and the replication rate, we found that the replication rate depended on not only the stability of the template DNA but also the topology of the structure of the template DNA. Furthermore, we identified that the changing the topology by molecular crowding conditions also affected the replication rate. These results imply the novel mechanism of the regulatino of biological reactions by the structures of nucleic acids.
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Report
(4 results)
Research Products
(26 results)
