• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of water-soluble Zinc porphyrin aimed at Theranostics

Research Project

Project/Area Number 26410189
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Bio-related chemistry
Research InstitutionUbe National College of Technology

Principal Investigator

HIROHARA SHIHO  宇部工業高等専門学校, 物質工学科, 教授 (70413804)

Co-Investigator(Renkei-kenkyūsha) OBATA Makoto  山梨大学, 総合研究部, 准教授 (70343267)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsポルフィリン亜鉛錯体 / がん診断法(PET) / がん治療法(PDT) / グルコース鎖 / メルカプトエタノール鎖 / Theranostics / がん治療(PDT) / がん診断(PDD) / セラノスティクス / 光細胞毒性試験 / ポルフィリン / 腫瘍集積性置換基 / 腫瘍集積時間促進置換基 / がん治療(PDT) / がん診断(PET) / Theragnosis / PET / PDT / ポルフィリン誘導体 / 糖鎖
Outline of Final Research Achievements

We synthesized [65Zn] or [Zn]-5,10,15,20-tetrakis(pentafluorophenyl)porphyrins ([65Zn]-P or Zn-P) bearing two kinds of substituent groups (G2M2), namely glucose group (denoted as G) and 2-mercaptoethanol group (denoted as M) as theranostics drug. The in vitro photocytotoxicity of Zn-PG2M2 was evaluated in HeLa human cervical cell line, RGK gastric carcinoma mucosal cell line, and two human glioblastoma cell lines (U87 and T98G). Zn-PG2M2 exerted the higher photocytotoxicity than fully glucosylated TFPP (G4) at 8 h incubation. From the pharmacokinetics study, the accumulation rate of [65Zn]-PG2M2 in the tumor was about the same as that in digestive organs at 24 h after intraperitoneal injection.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (34 results)

All 2017 2016 2015 2014 Other

All Journal Article (9 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Acknowledgement Compliant: 6 results) Presentation (21 results) (of which Int'l Joint Research: 3 results,  Invited: 4 results) Book (1 results) Remarks (3 results)

  • [Journal Article] Synthesis of Amphiphilic Block Copolymer consisting of Glycopolymer and Poly(L-lactide) and Preparation of Sugar-coated Polymer Aggregates2016

    • Author(s)
      M. Obata, R. Otobuchi, T. Kuroyanagi, M. Takahashi, S. Hirohara
    • Journal Title

      J. Polym. Sci. Part A: Polym. Chem.

      Volume: 55 Issue: 3 Pages: 395-403

    • DOI

      10.1002/pola.28401

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Syntheses, photophysical properties, and photocytotoxicities of tetrakis(fluorophenyl)porphyrin derivatives bearing 2-hydroxyethylthio groups2016

    • Author(s)
      M. Obata, S. Hirohara
    • Journal Title

      J. Photochem. Photobiol. B: Biol.

      Volume: 162 Pages: 324-331

    • DOI

      10.1016/j.jphotobiol.2016.07.006

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Effect of polymer matrix on the performance of pressure-sensitive paint comprising 5,10,15,20-tetrakis (pentafluorophenyl)porphinato platinum(II) and poly(1,1,1,3,3,3-hexafluoroisopropyl-co- tert-butyl methacrylates)2015

    • Author(s)
      M. Obata, R. Asato, S. Hirohara, K. Mitsuo
    • Journal Title

      J. Appl. Polym. Sci.

      Volume: xxx Issue: 16

    • DOI

      10.1002/app.43316

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis, Photophysical Properties, and Biological Evaluation of trans-Bisthioglycosylated Tetrakis(fluorophenyl)chlorin for Photodynamic Therapy2015

    • Author(s)
      S. Hirohara, C. Oka, M. Totani, M. Obata, J. Yuasa, H. Ito, M. Tamura, H. Matsui, K. Kakiuchi, T. Kawai, M. Kawaichi, M. Tanihara
    • Journal Title

      J. Med. Chem.

      Volume: 58 Issue: 21 Pages: 8658-8670

    • DOI

      10.1021/acs.jmedchem.5b01262

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] 光化学ガン医療のための糖連結ポルフィリンの開発2015

    • Author(s)
      廣原志保
    • Journal Title

      The 37th Anual Meeting of The Japanese Society for Photomedicine and Photobiology

      Volume: 37th Pages: 44-44

    • Related Report
      2015 Research-status Report
  • [Journal Article] Encapsulation of rat bone marrow stromal cells using a poly-ion complex gel of chitosan and succinylated poly(Pro–Hyp–Gly)2015

    • Author(s)
      Yuni Kusumastuti, Yoshiaki Shibasaki, Shiho Hirohara, Mime Kobayashi, Kayo Terada,Tsuyoshi Ando, Masao Tanihara
    • Journal Title

      J Tissue Eng Regen Med

      Volume: xxx Issue: 3 Pages: 869-876

    • DOI

      10.1002/term.1987

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] RAFT Synthesis of Statistical and Block Copolymers of 2-(α-D-Mannopyranosyloxy)ethyl Methacrylate with 2-(N,N-Dimethylamino)ethyl Methacrylate and Application for Nonviral Gene Delivery2015

    • Author(s)
      Makoto Obata, Tomoya Kobori, Shiho Hirohara, Masao Tanihara
    • Journal Title

      Polymer Chemistry

      Volume: 6 Issue: 10 Pages: 1793-1804

    • DOI

      10.1039/c4py01652a

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Synthesis of Fluoroporphyrin comprising Glucose and Ethylene Glycol and Evaluation of Its Photodynamic Effect2014

    • Author(s)
      Shiho Hirohara, Masahiro Shiraishi, Naoto Akiyama, Makoto Obata, Masato Tamura, Hiromu Ito, Hirofumi Matui, Kiyomi Kakiuchi
    • Journal Title

      ALA-Porphyrin Science

      Volume: 3(2) Pages: 61-69

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Synthesis and PDT Effect of Polysaccharide-conjugated Porphyrins2014

    • Author(s)
      Shiho Hirohara, Kohei Sharyo, Makoto Obata, Hayato Akahoshi, Masahiro Shiraishi, Kyosuke Fujitsu, Naoto Akiyama, Kenji Sugimoto, Kenji, Yoichi Nakano, Masato Tamura, Hirofumi Matsui and Masao Tanihara
    • Journal Title

      ALA-Porphyrin Science

      Volume: 3(1) Pages: 11-21

    • NAID

      40020244921

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 高専教員として私が工夫していること~教育と研究を両立させる方法~2017

    • Author(s)
      廣原志保
    • Organizer
      第17回日本化学会男女共同参画シンポジウム
    • Place of Presentation
      慶應義塾大学 日吉キャンパス
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ポルフィリンを用いたセラノスティクス薬剤の創成2017

    • Author(s)
      廣原志保
    • Organizer
      フォトダイナミックセラノスティクス研究会
    • Place of Presentation
      静岡大学浜松キャンパス
    • Year and Date
      2017-01-21
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] メルカプトエタノール連結クロリンの合成とPDT評価2016

    • Author(s)
      井町美晴、松村神奈、廣原志保、小幡誠、松井裕史
    • Organizer
      第31回日本酸化ストレス学会関東部会
    • Place of Presentation
      芝浦工業大学 豊洲キャンパス
    • Year and Date
      2016-12-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] Znポルフィリン誘導体の合成と物性評価2016

    • Author(s)
      松丸直睦、須子望加、廣原志保、伊藤紘、松井裕史
    • Organizer
      第31回日本酸化ストレス学会関東部会
    • Place of Presentation
      芝浦工業大学 豊洲キャンパス
    • Year and Date
      2016-12-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 抗酸化剤としてのポルフィリン金属錯体の合成と評価2016

    • Author(s)
      松村神奈、小幡誠、伊藤紘、松井裕史、廣原志保
    • Organizer
      第31回日本酸化ストレス学会関東部会
    • Place of Presentation
      芝浦工業大学 豊洲キャンパス
    • Year and Date
      2016-12-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 2つの置換基を有するフッ素クロリンの光細胞毒性2016

    • Author(s)
      廣原志保、小幡誠、松井裕史、垣内喜代三
    • Organizer
      第26回日本光線力学学会学術講演会(シンポジウム:フォトダイナミックセラノスティクスのためのポルフィリン薬剤の創成)
    • Place of Presentation
      はまぎんホール ヴィアマーレ(横浜)
    • Year and Date
      2016-06-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 光増感剤のための高分子キャリアの精密合成2016

    • Author(s)
      小幡誠、筒井雄也、廣原志保
    • Organizer
      第26回日本光線力学学会学術講演会(シンポジウム:フォトダイナミックセラノスティクスのためのポルフィリン薬剤の創成)
    • Place of Presentation
      はまぎんホール ヴィアマーレ(横浜)
    • Year and Date
      2016-06-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] 2つの置換基を持つクロリンの物性評価2016

    • Author(s)
      廣原志保、小幡誠、松井裕史、垣内喜代三
    • Organizer
      第6回ポルフィリンALA学会
    • Place of Presentation
      東京大学・医科学研究所
    • Year and Date
      2016-04-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] Mn ポルフィリンの合成と物性評価2016

    • Author(s)
      松村神奈、小幡誠、松井裕史、廣原志保
    • Organizer
      第6回ポルフィリンALA学会
    • Place of Presentation
      東京大学・医科学研究所
    • Year and Date
      2016-04-23
    • Related Report
      2016 Annual Research Report
  • [Presentation] PDT effect of TFPP derivatives having two different functional groups2015

    • Author(s)
      S. Hirohara, Y. Kubota, N. Akiyama, M. Obata, H. Matsui, K. Kakiuchi
    • Organizer
      The 3rd International ALA and Porphyrin Symposium (IAPS3)
    • Place of Presentation
      Hawai
    • Year and Date
      2015-12-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Synthesis and photocytotoxicity of fluorophyenylporphyrin derivatives having two different functional groups2015

    • Author(s)
      S. Hirohara, Y. Kubota, N. Akiyama, M. Obata, H. Matsui, K. Kakiuchi
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Bench to Bedside: Chemistry of Health Care, Hawai
    • Year and Date
      2015-12-16
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Synthesis and characterization of water-soluble polymers bearing porphyrin moiety at the initiating end for photodynamic therapy2015

    • Author(s)
      M. Obata, Y. Tsutsui, S. Hirohara
    • Organizer
      Pacifichem 2015
    • Place of Presentation
      Bench to Bedside: Chemistry of Health Care, Hawai
    • Year and Date
      2015-12-06
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] 光化学ガン医療のための糖連結ポルフィリンの開発2015

    • Author(s)
      廣原志保
    • Organizer
      第37回日本光医学・光生物学会
    • Place of Presentation
      宮崎
    • Year and Date
      2015-07-18
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 二つの水溶性置換基を有するポルフィリン誘導体の合成とin vitro評価2015

    • Author(s)
      廣原志保, 小幡誠, 松井裕史, 垣内喜代三
    • Organizer
      第25回日本光線力学学会
    • Place of Presentation
      東京
    • Year and Date
      2015-07-11
    • Related Report
      2015 Research-status Report
  • [Presentation] グルコース連結クロリンのPDT評価2014

    • Author(s)
      廣原志保, 谷原正夫, 岡千緒, 川市正史, 垣内喜代三, 小幡誠, 松井裕史
    • Organizer
      第29 回日本酸化ストレス学会関東支部会
    • Place of Presentation
      東京
    • Year and Date
      2014-12-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 2つの置換基を有する フッ素クロリンの合成と物性評価2014

    • Author(s)
      秋山直澄, 窪田雄介, 廣原志保, 小幡誠, 松井裕史, 谷本裕樹, 垣内喜代三
    • Organizer
      第29 回日本酸化ストレス学会関東支部会
    • Place of Presentation
      東京
    • Year and Date
      2014-12-20
    • Related Report
      2014 Research-status Report
  • [Presentation] 2つの水溶性置換基を有するポルフィリン誘導体の開発2014

    • Author(s)
      窪田雄介, 秋山直澄, 藤津恭介, 白石昌大, 赤星迅人, 小幡誠, 松井裕史, 廣原志保
    • Organizer
      2014年日本化学会中国四国支部大会
    • Place of Presentation
      山口
    • Year and Date
      2014-11-07 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] メルカプトエタノールとグルコースを有するクロリンの合成と細胞評価2014

    • Author(s)
      秋山直澄, 窪田雄介, 藤津恭介, 白石昌大, 赤星迅人, 小幡誠, 松井裕史, 廣原志保
    • Organizer
      2014年日本化学会中国四国支部大会
    • Place of Presentation
      山口
    • Year and Date
      2014-11-07 – 2014-11-08
    • Related Report
      2014 Research-status Report
  • [Presentation] グルコース鎖とエチレングリコール鎖を用いたポルフィリンの合成と細胞評価2014

    • Author(s)
      廣原志保, 白石昌大, 小幡誠, 松井裕史
    • Organizer
      第24回日本光線力学学会
    • Place of Presentation
      静岡
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2014 Research-status Report
  • [Presentation] メルカプトエタノールを連結したフッ素ポルフィリンの合成と光細胞毒性2014

    • Author(s)
      小幡誠, 廣原志保
    • Organizer
      第24回日本光線力学学会
    • Place of Presentation
      静岡
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2014 Research-status Report
  • [Presentation] ポルフィリン金属錯体のX線感受性試験2014

    • Author(s)
      廣原志保, 田村磨聖, 松井裕史, 小幡誠
    • Organizer
      第4回ポルフィリンALA学会
    • Place of Presentation
      神戸
    • Year and Date
      2014-04-26
    • Related Report
      2014 Research-status Report
  • [Book] Spectroscopic Tools for the Diagnosis and Treatment of Cancer2017

    • Author(s)
      N.Miyoshi, K.Hirakawa, H. Horiuchi, S.Okazaki, S-I. Ogura, M.Obata, S.Hirohara
    • Total Pages
      164
    • Publisher
      三恵社
    • Related Report
      2016 Annual Research Report
  • [Remarks]

    • URL

      http://www.ube-k.ac.jp/staff/chemical/

    • Related Report
      2016 Annual Research Report
  • [Remarks] USEARCH

    • URL

      https://www.ube-k.ac.jp/cms/wp-content/uploads/2015/03/HIROHARA-Shiho.pdf

    • Related Report
      2015 Research-status Report
  • [Remarks]

    • URL

      http://u-search.ube-k.ac.jp/tc-data/14672

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi