Quantification of post-translational modified amino acids by 2D-HPLC and ultra-sensitive amino acid analysis
| Project/Area Number |
26410190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | National Institute of Maritime, Port and Aviation Technology |
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Principal Investigator |
Masuda Akiko 国立研究開発法人海上・港湾・航空技術研究所, その他部局等, 研究員 (10322679)
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| Co-Investigator(Kenkyū-buntansha) |
堂前 直 国立研究開発法人理化学研究所, 環境資源科学研究センター, ユニットリーダー (00321787)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | アミノ酸分析 / 翻訳後修飾 / タンパク質 / 2次元クロマトグラフィー / 翻訳後修飾アミノ酸 / 固体酸触媒 / 加水分解 / アミノ酸 |
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| Outline of Final Research Achievements |
Proteins are chemically modified after translation based on genetic information of DNA, the modifications which are related to the expression of various functions of proteins. The modifications are referred to as post-translational modifications (PTMs). It is important to elucidate the relationship between PTMs and protein functions. In order to analysis of PTMs, modified amino acids themselves must be quantified since there is no description in DNA of what kind of modifications are executed. Until now, however, it has been difficult to quantify the modified amino acids because only small amounts of them are contained. In this study, quantitative hydrolysis method of proteins to amino acids and 2-dimensional HPLC to improve the resolution of analysis were developed. Combining these techniques with ultra-sensitive amino acid analysis, the analysis method was established for absolute quantification of modified amino acids.
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Report
(4 results)
Research Products
(7 results)
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[Journal Article] Structural basis for amino-acid export by DMT superfamily transporter YddG2016
Author(s)
H. Tsuchiya, S. Doki, M. Takemoto, T. Ikuta, T. Higuchi, K. Fukui, Y. Usuda, E. Tabuchi, S. Nagatoishi, K. Tsumoto, T. Nishizawa, K. Ito, N. Dohmae, R. Ishitani and O. Nureki
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Journal Title
Nature
Volume: 534
Issue: 7607
Pages: 417-420
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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