Effect of Hydration on Interfacial Interactions between DNA Brush Layers
| Project/Area Number |
26410232
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Polymer/Textile materials
|
|---|
| Research Institution | Shinshu University (2016-2017)
Institute of Physical and Chemical Research (2014-2015) |
|---|
Principal Investigator |
KANAYAMA Naoki 信州大学, 総合工学系研究科, 准教授(特定雇用) (80377811)
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | DNA / 界面 / 水和 / 界面間力 / フォースカーブ / AFM / 濡れ性 / イオン / 末端塩基対合 / 核酸 |
|---|
| Outline of Final Research Achievements |
Colloidal probe atomic force microscopy was employed to examine how the outermost base pairs affect the interaction between the dsDNA-grafted layers (dsDNA layers). The dsDNA layers having complementary (GC) or mismatched (CC) outermost base pairs were grafted onto the surfaces of colloidal probes and gold substrates. Force-distance curves measured in an aqueous medium under high-salt conditions revealed that the surface forces between the dsDNA layers were bilateral in nature and were governed by the outermost base pairs. Between complementary outermost dsDNA layers, the surface force changed from repulsive to attractive with an increase in the NaCl concentration (10-1000 mM). The attraction observed under high-salt conditions was attributed to the site-specific interaction proceeded only between complementary dsDNA terminals. In fact, between mismatched outermost dsDNA layers, the repulsive force was mostly dominant within the same NaCl concentration range.
|
Report
(5 results)
Research Products
(38 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] DNAで覆われたナノ粒子2016
Author(s)
金山直樹
Organizer
信州コロイド＆界面科学研究会 第2回研究討論会
Place of Presentation
信州大学 長野（工学）キャンパス、長野県
Year and Date
2016-10-28
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
