HIgh-efficiency and -accuracy cell separation using three-dimensional gradient AC electric field

• Project/Area Number: 26420130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Fluid engineering
• Research Institution: 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工学群)
• Principal Investigator: TADA Shigeru 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工, 応用科学群, 教授 (70251650)
• Co-Investigator(Kenkyū-buntansha): 塚本 哲 防衛大学校(総合教育学群、人文社会科学群、応用科学群、電気情報学群及びシステム工, 応用科学群, 講師 (90511460) ; 江口 正徳 一般財団法人ファジィシステム研究所, 研究部, 主任研究員 (60613594)
• Research Collaborator: Qiu Zhiyong
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 誘電泳動 / 細胞分離 / 対向式櫛型電極 / 数値シミュレーション / 交流電場 / ヒト正常細胞 / 出芽酵母

Outline of Final Research Achievements

In this study, a dielectrophoretic (DEP) cell-separation flow chamber having a parallel plate geometry was proposed. The flow chamber consisting of an interdigitated pair electrodes array at the bottom and a planar electrode on the top was developed to facilitate the separation of cells by creating a nonuniform AC electric field in the whole volume of the flow chamber. The operation and performance of the device was evaluated using live and dead yeast and Human epithermal breast (MCF10A) cells. The separation dynamics of suspended cells in the flow chamber was also investigated by simulating numerically the trajectories of individual cells under the action of combined DEP and dipole-dipole interparticle forces. Results demonstrated that cells were successfully separated with a remarkably high separation ratio under the appropriately-tuned field frequency. The numerically predicted behavior of the cell separation also showed a good agreement with those observed experimentally.

Research Products

• [Journal Article] Modeling and simulation of dielectrophoretic collective dynamics in a suspension of polarizable particles under the action of a gradient AC electric field (2017)
  • Author(s): Shigeru Tada, Yan Shen, Zhiyong Qiu
  • Journal Title: Electrophoresis Volume: 38 Issue: 11 Pages: 1434-1440
  • DOI: 10.1002/elps.201600572
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Enhancement of continuous-flow separation of viable/nonviable yeast cells using a nonuniform alternating current electric field with complex spatial distribution (2016)
  • Author(s): S. Tada, A. Nakanishi, M. Eguchi, K. Ochi, M. Baba, A. Tsukamoto
  • Journal Title: Biomicrofluidics Volume: 10 Issue: 3
  • DOI: 10.1063/1.4950999
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Concentration-tuned interactions and structures in flowing suspensions under a gradient AC electric field (2016)
  • Author(s): Shigeru Tada, Zhiyong Qiu, Yan Shen
  • Journal Title: Journal of Applied Physics Volume: 120 Issue: 17
  • DOI: 10.1063/1.4966567
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Numerical simulation of dielectrophoretic separation of live/dead cells using a three-dimensional nonuniform AC electric field in micro-fabricated devices (2015)
  • Author(s): Shigeru Tada
  • Journal Title: Biorheology Volume: 52 Issue: 3 Pages: 211-224
  • DOI: 10.3233/bir-14039
  • Peer Reviewed
• [Journal Article] Dielectric aggregation kinetics of cells in a uniform AC electric field (2015)
  • Author(s): Shigeru Tada, Tomoyuki Natsuya, Akira Tsukamoto
  • Journal Title: Biorheology Volume: 51 Issue: 6 Pages: 381-397
  • DOI: 10.3233/bir-14034
  • Peer Reviewed
• [Presentation] 三次元不均一電場での誘電泳動による細胞分離 (2017)
  • Author(s): 多田 茂, 林 雅子, 逢見優衣, 塚本 哲
  • Organizer: 第29回バイオエンジニアリング講演会
  • Place of Presentation: 名古屋, ウィンク愛知
• [Presentation] 誘電泳動による細胞分離の高効率化 (2016)
  • Author(s): 多田 茂, 中西 有咲, 大地 健吾, 塚本 哲
  • Organizer: 第28回バイオエンジニアリング講演会
  • Place of Presentation: 東京工業大学大岡山キャンパス
  • Year and Date: 2016-01-10
• [Presentation] 三次元不均一交流電界を用いた細胞分離 (2015)
  • Author(s): 多田 茂, 大地健吾, 塚本 哲
  • Organizer: 第27回バイオエンジニアリング講演会
  • Place of Presentation: 新潟県，新潟市
  • Year and Date: 2015-01-10
• [Presentation] Separation of Live/Dead Cells by the Use of Three Dimensional Non-uniform AC Electric Field (2014)
  • Author(s): Shigeru Tada
  • Organizer: World Automation Congress 2014
  • Place of Presentation: Hawaii, USA
  • Year and Date: 2014-08-04
• [Remarks] 防衛大学校 応用科学群 応用物理学科 応用数理生理学研究室
  • URL: http://www.nda.ac.jp/cc/biomed/index.html