An Investigation on a Light Waveguide Implemented Optical Total Analysis System for Internal Cell Structure Analysis
| Project/Area Number |
26420210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Intelligent mechanics/Mechanical systems
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| Research Institution | Toyo University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
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| Keywords | TAS（総合分析システム） / マイクロ流路 / 光導波路 / 蛍光検出 / FDTD法 / BPM法 / 光スキャナー / TAS / 照射角度走査 / 光スキャナ / FDTD 法 / 前方散乱光 / 側方散乱光 |
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| Outline of Final Research Achievements |
The purpose of our research is to estimate distribution of fluorescent substance inside cells or micro particles utilizing directivity of emitted fluorescence from such micro objects. In order to obtain precise directivity, we utilized a transparent resin material and fabricated it as cell-sized micro fluidic channels and light waveguides formed on a monolithic chip. We prepared three stages of development steps: they were, to obtain extremely weak fluorescence with higher signal-to-noise ratio, to obtain fluorescence by switching light waveguides sequentially, and finally, to detect fluorescence by changing illuminating angle sequentially. Three goals could have almost been achieved, and as a result, we have succeeded in obtaining directivity of a 5-μm-diameter resin particle with uniformly distributed fluorescent substance.
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Report
(4 results)
Research Products
(12 results)
