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An Investigation on a Light Waveguide Implemented Optical Total Analysis System for Internal Cell Structure Analysis

Research Project

Project/Area Number 26420210
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Intelligent mechanics/Mechanical systems
Research InstitutionToyo University

Principal Investigator

Ohkubo Toshifumi  東洋大学, 理工学部, 教授 (60349933)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
KeywordsTAS(総合分析システム) / マイクロ流路 / 光導波路 / 蛍光検出 / FDTD法 / BPM法 / 光スキャナー / TAS / 照射角度走査 / 光スキャナ / FDTD 法 / 前方散乱光 / 側方散乱光
Outline of Final Research Achievements

The purpose of our research is to estimate distribution of fluorescent substance inside cells or micro particles utilizing directivity of emitted fluorescence from such micro objects. In order to obtain precise directivity, we utilized a transparent resin material and fabricated it as cell-sized micro fluidic channels and light waveguides formed on a monolithic chip. We prepared three stages of development steps: they were, to obtain extremely weak fluorescence with higher signal-to-noise ratio, to obtain fluorescence by switching light waveguides sequentially, and finally, to detect fluorescence by changing illuminating angle sequentially. Three goals could have almost been achieved, and as a result, we have succeeded in obtaining directivity of a 5-μm-diameter resin particle with uniformly distributed fluorescent substance.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Open Access: 3 results,  Acknowledgement Compliant: 3 results) Presentation (5 results) (of which Int'l Joint Research: 3 results) Remarks (4 results)

  • [Journal Article] Evaluation of fluorescence emitting characteristics of a microparticle by illumination angle scanning utilizing a resin-based monolithic TAS chip2017

    • Author(s)
      Toshifumi Ohkubo, Nobuyuki Terada, Yoshikazu Yoshida
    • Journal Title

      Microsystem Technologies, Springer

      Volume: 印刷中 Issue: 11 Pages: 5149-5162

    • DOI

      10.1007/s00542-016-3199-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Preliminary scanning fluorescence detection of a minute particle running along a waveguide implemented microfluidic channel using a light switching mechanism2016

    • Author(s)
      Toshifumi. Ohkubo, Nobuyuki. Terada, Yoshikazu. Yoshida
    • Journal Title

      Microsystem Technologies, Springer

      Volume: 未定 Issue: 6 Pages: 1227-1240

    • DOI

      10.1007/s00542-016-2844-0

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Fluorescence detection of minute particles using a resin-based optical total analysis system with a high-aspect-ratio light waveguide core2015

    • Author(s)
      Toshifumi. Ohkubo, Nobuyuki. Terada, Yoshikazu. Yoshida
    • Journal Title

      Microsystem Technologies, Springer

      Volume: 印刷中 Issue: 12 Pages: 2611-2622

    • DOI

      10.1007/s00542-015-2505-8

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Evaluation of fluorescence emitting characteristics of a microparticle by illumination angle scanning utilizing a resin-based monolithic TAS chip2016

    • Author(s)
      Toshifumi Ohkubo, Nobuyuki Terada, Yoshikazu Yoshida
    • Organizer
      The 14th International Symposium on Bio-Science and Nano Technology
    • Place of Presentation
      Toyo University, Kawagoe, Saitama 350-8585, JAPAN
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of fluorescence emitting characteristics of a micro particle by illumination angle scanning utilizing a resin-based monolithic TAS chip2016

    • Author(s)
      Toshifumi Ohkubo, Nobuyuki Terada, Yoshikazu  Yoshida
    • Organizer
      ASME 2016 Conference on Information Storage and Processing Systems, ISPS2016
    • Place of Presentation
      Santa Clara (Mariott), California, USA
    • Year and Date
      2016-06-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Preliminary scanning fluorescence detection of a minute particle running along a waveguide implemented microfluidic channel using a light switching mechanism2015

    • Author(s)
      Toshifumi. Ohkubo, Nobuyuki. Terada, Yoshikazu. Yoshida
    • Organizer
      2015 JSME-IIP/ASME-ISPS Joint Integration Conference on Micromechatronics for Information and Precision Equipment, MIPE2015
    • Place of Presentation
      Kobe International Conference Center, Kobe, Japan
    • Year and Date
      2015-06-15
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Fluorescence detection of minute particles using a resin-based optical total analysis system with a high-aspect-ratio light waveguide core2014

    • Author(s)
      Toshifumi. Ohkubo, Nobuyuki. Terada, Yoshikazu. Yoshida
    • Organizer
      The 12th International Symposium on Bio-Science and Nano Technology
    • Place of Presentation
      Toyo University, Kawagoe, Saitama 350-8585, JAPAN
    • Year and Date
      2014-11-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Fluorescence detection of minute particles using a resin-based optical total analysis system with a high-aspect-ratio light waveguide core2014

    • Author(s)
      Toshifumi. Ohkubo, Nobuyuki. Terada, Yoshikazu. Yoshida
    • Organizer
      ASME(米国機械学会)、JSME(日本機械学会)共催国際会議ISPS2014
    • Place of Presentation
      Santa Clara University, San Jose, California, USA
    • Year and Date
      2014-06-24
    • Related Report
      2014 Research-status Report
  • [Remarks] 東洋大学研究者情報データベース

    • URL

      http://ris.toyo.ac.jp/profile/ja.5efeabbf9c86c31faf262871afbb8730.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 東洋大学大学院 理工学研究科 機能システム専攻 教員紹介

    • URL

      http://ris.toyo.ac.jp/profile/ja.FR21Va3TZP42g0heFsX.XQ==.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 東洋大学 理工学部 機械工学科 マイクロメカトロニクス研究室

    • URL

      http://www.toyo.ac.jp/site/dmec/24233.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 東洋大学 理工学研究科機能システム専攻の教員紹介

    • URL

      http://ris.toyo.ac.jp/profile/ja.FR21Va3TZP42g0heFsX.XQ==.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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