| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
A measurement system for surface plasmon-field enhanced fluorescence spectroscopy (SPFS) was developed. A laser diode as a light source was employed in the optical system. A convergent light beam was irradiated from the laser diode to the interface between a prism and an Au thin film. Fluorescent dye was excited by surface plasmon resonance (SPR) and fluorescence emission measured by a photomultiplier tube. Specific antibody-antigen interaction between fluorescent labeled-anti-trinitrotoluene (TNT) monoclonal antibody and a TNT analog immobilized on the Au surface or TNT could be monitored. Additionally, a TNT analog was immobilized on a polymer-modified sensor surface. TNT was measured by a displacement method on an SPR system, and a LOD achieved over the sensor chip was 0.9 ng/mL. Furthermore, inverse opal photonic crystal (IOPC) films modified with different six alkoxysilanes were developed as odor-responsive films. Three gases could be identified using the IOPC films.
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