| Project/Area Number |
26420534
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Civil and environmental engineering
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| Research Institution | Prefectural University of Hiroshima |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 嫌気性芽胞菌 / ウェルシュ菌 / ノロウイルス / PMMoV / 糞便汚染指標 / ソーストラッキング指標 / 毒素遺伝子 / 大腸菌 / 海域 |
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| Outline of Final Research Achievements |
In this study, the efficacy of anaerobic spores (Clostridium perfringens) as indicator for intestinal virus and microbial source tracking were investigated.
Concentrations of C. perfringens in coastal sea water were significantly correlated between Norovirus G2. C. perfingens were detected from all samples of Norovirus positive (11 samples), whereas, E. coli were only detected from only 4 of 11 Norovirus positive samples. Because of cpe positive C. perfringens isolates were detected from only human related wastewater samples, detection of cpe positive isolates were useful microbial source tracking indicator as human fecal pollution of surface waters. Concentration of Erythromycin in Handford Agar, which used for separation culture of C. perfringens from water samples, affected to growth and detection of C. perfringens.
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