Creation of material for regenerative medicine by supercritical carbon dioxide combined with ultrasonic wave

• Project/Area Number: 26420770
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Properties in chemical engineering process/Transfer operation/Unit operation
• Research Institution: Fukuoka University
• Principal Investigator: MISHIMA Kenji 福岡大学, 工学部, 教授 (40190623)
• Co-Investigator(Kenkyū-buntansha): 井上 俊孝 西九州大学, 健康福祉学部, 教授(移行) (20274615) ; 志村 英生 福岡大学, 医学部, 教授 (80178996)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
• Keywords: 再生医療 / 超臨界 / 二酸化炭素 / 材料 / 化学工学

Outline of Final Research Achievements

In this study, we developed a method of creating a regenerative medical material (a new bio-scaffold matrix) using supercritical carbon dioxide to irradiate tissues of different mammals such as pigs with ultrasonic waves to remove cellular constituents that can be used for regenerative medicine. Using a decellularized tissue of living tissue, a information of the tissue structure, such as a cross-sectional photograph was collected as data by micro X-ray CT. In order to make this a commercially available device, we succeeded in reproducing the decellularized tissue with the structure data obtained by micro X-ray CT data using the 3D printer.

Research Products

• [Journal Article] Enhancement and regulation effect of myrcene on antibody response in immunization with ovalbumin and Ag85B in mice (2017)
  • Author(s): 4)Saori Uyeda, Tanjina Sharmin, Tomomitsu Satho, Keiichi Irie, Mineo Watanabe, Masato Hosokawa, Yukihiro Hiramatsu, Takayuki Koga, Yukihiko Nakashima, Nobuhiro Kashige, Akihisa Toda, Kenji Mishima, Fumio Miake
  • Journal Title: Asian Pacific Journal of Allergy and Immunology Volume: 印刷中
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Extraction of Isovitexin from Melinjo (Gnetum Gnemon L.) Leaves Using Mixtures of Liquid Carbon Dioxide and Ethanol (2016)
  • Author(s): Yusraini Dian Inayati Siregar, Kenji Mishima, et. al.
  • Journal Title: Int. J. of Biomass and Renewables Volume: 5 Pages: 23-30
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Effect of Simple Calculation Exercise on Cerebral Blood Oxygenation During a Verbal Memory Task Measured by fNIRS (2016)
  • Author(s): Misumi, M., K. Mishima, H. Orii, T. Sharmin, S.Tokunaga, M.Haraguchi, M.Nakamura, T.Harada, T.Suetsugu,K.Irie, K.Mishima, T.Satho
  • Journal Title: J.Inst. Ind. Appli. Eng. Volume: 4 Pages: 199-205
  • NAID: 40021221730
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Functional Ingredients Extraction from Garcinia Mangostana Pericarp by Liquefied Dimethyl Ether (2016)
  • Author(s): Nerome, R. Hoshino, S. Ito, Ryohey E., Y. Eto, S. Wakiyama, T. Sharmin, M. Goto, H. Kanda, K. Mishima
  • Journal Title: Engineering Journal Volume: 20
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Bag of Visual-WordsとSVMを用いた絵画の分類 (2016)
  • Author(s): 7)三角 真、折居 英章、Tanjina SHARMIN、三島健司、鶴岡 知昭
  • Journal Title: 福岡大学工学集報 Volume: 96
  • Acknowledgement Compliant
• [Journal Article] fNIRSによる音楽聴取時の前頭前野における脳血液量の推測と考察 (2016)
  • Author(s): 8)三角 真、折居 英章、Tanjina SHARMIN、三島健司、西原 宏
  • Journal Title: 福岡大学工学集報 Volume: 96 Pages: 25-28
  • Acknowledgement Compliant
• [Journal Article] Ultrasonically enhanced extraction of luteolin and apigenin from the leaves of Perilla frutescens (L.) Britt. using Liquid carbon dioxide and ethanol (2016)
  • Author(s): Hirofumi Kawamura, Kenji Mishima, Tanjina Sharmin, Shota Ito, Ryo Kawakami, Takafumi Kato, Makoto Misumi, Tadashi Suetsugu, Hideaki Orii, et. al.
  • Journal Title: Ultrasonics Sonochemistry Volume: 29 Pages: 19-26
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Gas-saturated solution process to obtain microcomposite particles of alpha lipoic acid/hydrogenated colza oil in supercritical carbon dioxide (2016)
  • Author(s): Kenji Mishima, Masatoshi Honjo, Tanjina Sharmin, Shota Ito, Ryo Kawakami, Takafumi Kato, Makoto Misumi, Tadashi Suetsugu, Hideaki Orii, Hiroyuki Kawano, Keiichi Irie, Kazunori Sano, Kenichi Mishima, Takunori Harada, and Mikio Ouchi
  • Journal Title: Pharmaceutical Development and Technology Volume: 21(6) Pages: 37-48
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Enhancement and regulation effect of myrcene on antibody response in immunization with ovalbumin and Ag85B in mice (2016)
  • Author(s): Saori Uyeda, Tanjina Sharmin, Tomomitsu Satho, Keiichi Irie, Mineo Watanabe, Masato Hosokawa, Yukihiro Hiramatsu, Takayuki Koga, Yukihiko Nakashima, Nobuhiro Kashige, Akihisa Toda, Kenji Mishima, Fumio Miake
  • Journal Title: Asian Pacific Journal of Allergy and Immunology Volume: in press
  • Peer Reviewed
• [Journal Article] Bag of Visual-WordsとSVMを用いた絵画の分類 (2016)
  • Author(s): 三角 真、折居 英章、Tanjina SHARMIN、三島健司､鶴岡 知昭
  • Journal Title: 福岡大学工学集報 Volume: 96
• [Journal Article] Ultrasonically enhanced extraction of luteolin and apigenin from the leaves of Perilla frutescens (L.) Britt. using Liquid carbon dioxide and ethano (2016)
  • Author(s): Hirofumi Kawamura, Kenji Mishima, Tanjina Sharmin, Shota Ito, Ryo Kawakami, Takafumi Kato, Makoto Misumi, Tadashi Suetsugu, Hideaki Orii, Hiroyuki Kawano, Keiichi Irie, Kazunori Sano, Kenichi Mishima, Takunori Harada,
  • Journal Title: Ultrasonics Sonochemistry, Volume: 29
  • Peer Reviewed / Open Access
• [Journal Article] Extraction of Apigenin and Luteolin from Leaves of Alfalfa (Medicago sativa L.) Using Mixtures of Liquid Carbon Dioxide and Ethanol (2015)
  • Author(s): Kenji MISHIMA, Hirofumi KAWAMURA, Ryo KAWAKAMI, Shota ITO, Yuuta INOUE, Tetsuya HIROTA, Takunori HARADA, Takafumi KATO, Tanjina SHARMIN, Makoto MISUMI,Tadashi SUETSUGU, Keiichi IRIE, Kenichi MISHIMA, Salim MUSTOFA, Fauziyah HASANAH,Yusraini Dian Inayati SIEGAR, Hilyatuz ZAHARO, Lily Surayya Eka PUTRI, Agus SALIM
  • Journal Title: Solvent Extr. Res. Dev., Jpn Volume: 22 Pages: 177-186
  • NAID: 130005071512
  • Peer Reviewed / Open Access
• [Journal Article] Liquid Carbon Dioxide Extraction of Xanthones from the Pericarps of Garcinia Mangostana Linn. Enhanced by Ultrasonic Irradiation. (2015)
  • Author(s): 7)Kenji MISHIMA, Hirofumi KAWAMURA, Shota ITO, Yuuta INOUE, Tetsuya HIROTA, Takafumi KATO, Tanjina SHARMIN, Ryo KAWAKAMI, Keiichi IRIE, Kenichi MISHIMA, Takunori HARADA, Salim MUSTOFA, Fauziyah HASANAH,Yusraini Dian Inayati SIEGAR, Lily Surayya Eka PUTRI, Agus SALIM
  • Journal Title: Solvent Extr. Res. Dev., Jpn Volume: 22 Pages: 187-199
  • NAID: 130005071513
  • Peer Reviewed / Open Access
• [Journal Article] Extraction of Resveratrol from Melinjo (Gnetum gnemon L.) Seeds Using Mixtures of Liquid Carbon Dioxide and Ethanol (2015)
  • Author(s): Kenji Mishima, Yusraini Dian Inayati SIREGAR, Hirofumi KAWAMURA, Ryo KAWAKAMI, Shota ITO, Yuuta INOUE, Tetsuya HIROTA, Takunori HARADA, Takafumi KATO, Makoto MISUMI, Tadashi SUETSUGU, Keiichi IRIE, Kenichi MISHIMA, Salim MUSTOFA, Fauziyah HASANAH, Hilyatuz ZAHARO, Lily Surayya Eka PUTRI and Agus SALIM
  • Journal Title: Solvent Extr. Res. Dev., Jpn. Volume: 22 Pages: 69-77
  • NAID: 130005071503
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Extraction of Apigenin and Luteolin from Leaves of Alfalfa (Medicago sativa L.) Using Mixtures of Liquid Carbon Dioxide and Ethanol (2015)
  • Author(s): Kenji MISHIMA, Hirofumi KAWAMURA, Ryo KAWAKAMI, Shota ITO, Yuuta INOUE, Tetsuya HIROTA, Takunori HARADA, Takafumi KATO, Tanjina SHARMIN, Makoto MISUMI,Tadashi SUETSUGU, Keiichi IRIE, Kenichi MISHIMA, Salim MUSTOFA, Fauziyah HASANAH,Yusraini Dian Inayati SIEGAR, Hilyatuz ZAHARO, Lily Surayya Eka PUTRI, Agus SALIM
  • Journal Title: Solvent Extr. Res. Dev., Jpn. Volume: 22 Pages: 177-186
  • NAID: 130005071512
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Liquid Carbon Dioxide Extraction of Xanthones from the Pericarps of Garcinia Mangostana Linn. Enhanced by Ultrasonic Irradiation (2015)
  • Author(s): Kenji MISHIMA, Hirofumi KAWAMURA, Shota ITO, Yuuta INOUE, Tetsuya HIROTA, Takafumi KATO, Tanjina SHARMIN, Ryo KAWAKAMI, Makoto MISUMI,Tadashi SUETSUGU, Keiichi IRIE, Kenichi MISHIMA, Takunori HARADA, Salim MUSTOFA, Fauziyah HASANAH,Yusraini Dian Inayati SIEGAR, Lily Surayya Eka PUTRI, Agus SALIM
  • Journal Title: Solvent Extr. Res. Dev., Jpn. Volume: 22 Pages: 187-199
  • NAID: 130005071513
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Gas-saturated solution process to obtain microcomposite particles of alpha lipoic acid/hydrogenated colza oil in supercritical carbon dioxide (2015)
  • Author(s): Kenji Mishimaa, Masatoshi Honjo, Tanjina Sharmin, Shota Ito, Ryo Kawakami, Takafumi Kato, Makoto Misumi, Tadashi Suetsugu, Hideaki Orii, Hiroyuki Kawano, Keiichi Irie, Kazunori Sano, Kenichi Mishima, Takunori Harada, and Mikio Ouchi
  • Journal Title: Pharmaceutical Development and Technology Volume: 60 Pages: 111-222
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Plasmon-Resonance-Enhanced CPL for Self-Assembly Meso-tetrakis(4-sulfonatophenyl)porphyrin-Surfactant Complex interacted with Ag nanoparticles (2014)
  • Author(s): Takunori Harada, Naoki Kajiyama, Kei Ishizaka, Reona Toyofuku,b Katsuki Izumi,b Kazuo Umemura, Naoya Taniguchic and Kenji Mishima Yoshitane Imai,
  • Journal Title: Chem. Commun. Volume: 50 Issue: 76 Pages: 11173-11176
  • DOI: 10.1039/c4cc04477k
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Spectroscopic Characterization of Supramolecular Chiral Porphyrin Homoassociates at the Air-Water Interface (2014)
  • Author(s): Takunori Harada, Hiroshi Moriyama, Hiromi Takahashi, Kazuo Uemura, Haruo Yokota, Ryo Kawakami and Kenji Mishima
  • Journal Title: Appl. Spectrosc. Volume: 68(11) Pages: 1235-1240
  • Peer Reviewed / Open Access
• [Presentation] 超臨界二酸化炭素を用いた九州産植物の有効成分抽出と脳機能評価 (2017)
  • Author(s): 三島健司
  • Organizer: 第３回Japan Cosmetic Center(JCC)産学交流セミナー
  • Place of Presentation: 唐津市
  • Year and Date: 2017-03-01
  • Invited
• [Presentation] ナノ・マイクロカプセルの製造と医療・健康食品・化粧品への応用 (2016)
  • Author(s): 三島健司
  • Organizer: 日本材料学会九州支部第25回技術懇話会,
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2016-12-09
  • Invited
• [Presentation] Bringing technology of nano and micro materials to cosmetic and drug industry (2016)
  • Author(s): K. Mishima
  • Organizer: 2016 International Cosmetic-Tech Conference & Exhibition
  • Place of Presentation: Chienkuo Technology University, Taiwan
  • Year and Date: 2016-10-20
  • Int'l Joint Research / Invited
• [Presentation] Bringing Research to Industry Environmentally Benign Commercialization of Supercritical Fluid Technology (2016)
  • Author(s): K. Mishima
  • Organizer: 2nd international conference on industrial pharmacy
  • Place of Presentation: Kuantan, Malaysia
  • Year and Date: 2016-08-15
  • Int'l Joint Research / Invited
• [Presentation] Novel Products and Evaluation Methods for Cosmetics and Medicine in Asian Network (2016)
  • Author(s): K. Mishima
  • Organizer: 2016 International Symposium on Cosmetic Regulation
  • Place of Presentation: Taipei International Convention Center, Taipei, Taiwan
  • Year and Date: 2016-08-03
  • Int'l Joint Research / Invited
• [Presentation] ナノ・マイクロカプセルの製造と医療・健康食品・化粧品への応用 (2016)
  • Author(s): 三島健司
  • Organizer: M&M研究会
  • Place of Presentation: M&M研究会（サンメッセ鳥栖）
  • Year and Date: 2016-07-22
  • Invited
• [Presentation] Functional Ingredients Extraction from Garcinia Mangostana Pericarp by Liquefied Dimethyl Ether. (2015)
  • Author(s): Hazuki Nerome, Rintaro Hoshino, Shota Ito, Tanjina Sharmin, Hideki Kanda, Motonobu Goto, Kenji Mishima
  • Organizer: the 13th International Symposium on East Asian Resources Recycling Technology (EARTH 2015)
  • Place of Presentation: Dusit Thani Pattaya, Thailand
  • Year and Date: 2015-11-01
  • Int'l Joint Research
• [Presentation] 超臨界二酸化炭素に対するバイカレインの溶解度の測定ならびに相関 (2015)
  • Author(s): 2)三島健司,伊藤祥太,川上亮,タンジナ・シャルミン,佐伯優太,戸花優介, 平山沙織,加藤貴史,三角真,末次正,折居英章,入江圭一,佐野和憲,三島健一,原田拓典,河村弘文
  • Organizer: 第34回溶媒抽出討論会
  • Place of Presentation: 金沢工大
  • Year and Date: 2015-10-23
• [Presentation] Effect of Lightning Impulse Discharge on PVC Thin Film. (2015)
  • Author(s): 3)Norimitsu Takamura, Takao Matsumoto, Hazuki Nerome, Yasuji Izawa, Kenji Mishima, Masahiro Hanai, Kiyoto Nishijima,
  • Organizer: 9th International Conference on Reactive Plasma
  • Place of Presentation: Hawaii Convention Center
  • Year and Date: 2015-10-12
  • Int'l Joint Research
• [Presentation] 生体に優しい二酸化炭素の機能性溶媒としての利用 (2015)
  • Author(s): K. Mishima
  • Organizer: 産業応用工学会全国大会2015
  • Place of Presentation: 福岡大学
  • Year and Date: 2015-09-04
  • Invited
• [Presentation] 超臨界二酸化炭素の圧力誘起法を用いたpH応答性高分子マイクロコーティング (2014)
  • Author(s): 伊藤祥太, 三島健司, 原田拓典, 入江圭一, 三島健一
  • Organizer: SCEJ化学工学会第46回秋季大会(2014.9.17-19)
  • Place of Presentation: 九州大学
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] 液体二酸化炭素によるアルファルファ葉からのルテオリンとアピゲニンの抽出 (2014)
  • Author(s): 河村弘文, 三島健司, 原田拓典, 入江圭一, 川上亮, 三島健一
  • Organizer: SCEJ化学工学会第46回秋季大会(2014.9.17-19)
  • Place of Presentation: 九州大学
  • Year and Date: 2014-09-17 – 2014-09-19
• [Presentation] オキサリプラチン誘発性末梢神経障害ラットのCharcot-Marie-Tooth病原因遺伝子の動態 (2014)
  • Author(s): 寺岡里織、入江圭一、山下郁太、岩橋理紗、吉武摩耶、明瀬孝之、原暦、百武美香、市丸優希、林稔展、松尾宏一、佐藤朝光、三島健司、岩崎克典、三島健一
  • Organizer: 第52回日本癌治療学会学術集会(2014.8.28-30)パシフィコ横浜
  • Place of Presentation: 横浜
  • Year and Date: 2014-08-28 – 2014-08-30
• [Presentation] Environmentally Benign Application of Supercritical Fluid Technology to Advanced Production of Materials (2014)
  • Author(s): K. Mishima
  • Organizer: 2nd International Conference on Ecology, Sanitation, Water, Resources, Materials and Energy
  • Place of Presentation: Fukuoka University
  • Year and Date: 2014-08-23 – 2014-08-27
  • Invited
• [Presentation] Interaction of different polyphenols with serum albumins: Spectroscopic and molecular modelling studies (2014)
  • Author(s): Hilyatuz ZAHROH, Kenji MISHIMA, Takunori HARADA, Shouta ITOU, Keiichi IRIE,Kenichi MISHIMA, Yusraini Dian Inayati SIREGAR, Lily Surayya Eka PUTRI
  • Organizer: 2nd International Conference on Ecology, Sanitation, Water, Resources, Materials and Energy (ESWRME)(2014.8.23-27) Fukuok
  • Place of Presentation: Fukuoka University
  • Year and Date: 2014-08-23 – 2014-08-27
• [Presentation] Extraction of Apigenin and Luteolin and from Leaves of Medicago sativa (L.) with Liquid Carbon Dioxide (2014)
  • Author(s): Hirofumi KAWAMURA, Kenji MISHIMA, Ryo KAWAKAM1, Shouta ITOU, Tetsuya Hirota, Takunori HARADA, Takafumi KATO, Keiichi IRIE, Kenichi MIHIMA, Salim Mustofa, Fauziyah HASANAH, Yusraini Dian Inayati SIREGAR, Hilyatuz ZAHARO, Lily Surayya Eka PUTRI, Agus SALIM
  • Organizer: 2nd International Conference on Ecology, Sanitation, Water, Resources, Materials and Energy (ESWRME)(2014.8.23-27) Fukuok
  • Place of Presentation: Fukuoka University
  • Year and Date: 2014-08-23 – 2014-08-27
• [Presentation] Formation of pH-release microcapusles in supercritical carbon dioxide (2014)
  • Author(s): Shouta ITOU, Yusraini Dian Inayati SIREGAR, Kenji MISHIMA, Ryo KAWAKAMI, Yuuta INOUE, Hirofumi KAWAMURA, Takunori HARADA, Keiichi IRIE, Kenichi MIHIMA, Salim Mustofa, Fauziyah HASANAH, Hilyatuz ZAHAROH, Lily Surayya Eka PUTRI, Agus SALIM
  • Organizer: 2nd International Conference on Ecology, Sanitation, Water, Resources, Materials and Energy (ESWRME)(2014.8.23-27) Fukuok
  • Place of Presentation: Fukuoka University
  • Year and Date: 2014-08-23 – 2014-08-27
• [Presentation] Extraction of Flavonoids from Melinjo (Gnetum gnemon L.) Leaves with Liquid Carbon Dioxide and Ethanol (2014)
  • Author(s): Yusraini Dian Inayati SIREGAR, Kenji MISHIMA, Ryo KAWAKAMI, Shouta ITOU, Yuuta INOUE, Takunori HARADA, Keiichi IRIE, Kenichi Mishima, Hirofumi KAWAMURA, Hilyatuz Zahroh, Lily Surayya Eka PUTRI and Agus SALIM
  • Organizer: 2nd International Conference on Ecology, Sanitation, Water, Resources, Materials and Energy (ESWRME)(2014.8.23-27) Fukuoka
  • Place of Presentation: Fukuoka University
  • Year and Date: 2014-08-23 – 2014-08-27
• [Presentation] Microencapsulation of Particles with Fluoropolymer by Pressure-Induced Phase Separation of Supercritical Carbon Dioxide Solutions (2014)
  • Author(s): K. Mishima
  • Organizer: he 4th International Symposium on Aqua Science and Water Resources (ISASWR2014)
  • Place of Presentation: Shanxi University
  • Year and Date: 2014-08-13 – 2014-08-16
  • Invited
• [Presentation] 円偏光吸収および円偏光発光測定システムの開発 (2014)
  • Author(s): 原田拓典、髙本 真、梶山直樹、早川広志、渡辺正行、三島健司
  • Organizer: Symposium on Molecular Chirality 2014(2014.6.6-7 Sendai)
  • Place of Presentation: 仙台
  • Year and Date: 2014-06-06 – 2014-06-07
• [Presentation] 表面プラズモン共鳴増強によるキラル光学特性評価 (2014)
  • Author(s): 梶山直樹、石坂慶、梅村和夫、谷口直哉、今井喜胤、三島健司、原田拓典
  • Organizer: Symposium on Molecular Chirality 2014(2014.6.6-7 Sendai)
  • Place of Presentation: 仙台
  • Year and Date: 2014-06-06 – 2014-06-07
• [Book] Professional Engineer Library 物理化学 (2015)
  • Author(s): 福地賢治、三島健司ら
  • Total Pages: 256
  • Publisher: 実教出版