Imobilization of scFv through SiN-binding peptide for sensitive RIfS sensor
| Project/Area Number |
26420795
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
Kumada Yoichi 京都工芸繊維大学, 分子化学系, 准教授 (70452373)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | SiN / SiN-tag / antibody / GST / immobilization / orientation control / 窒化ケイ素 / 単鎖抗体 / 材料親和性ペプチド / 配向制御 / リフォールディング / 固定化 / バイオセンサ |
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| Outline of Final Research Achievements |
In this study, we developed a new immobilization method of protein onto the surface of silicone nitride. First, we successfully identified SIN-binding peptides from Elongation Factor Tu which was isolated from E. coli cell lysate. Especially, one of them showed the strongest binding affintiy towards the surface of SIN plate. By genetic fusion of SIN-tag to the C-termimus of single-chain Fv antibodies resulted in higher antigen-binding activity of scFv in adsorption state. Thus, the SIN-binding peptides developed in this study will be significantly useful for site-specific immobilization of recombinant antibody fragments such as scFvs in RIfS sensor.
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Report
(4 results)
Research Products
(3 results)
