| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Outline of Final Research Achievements |
Lignan is a class of useful plant secondary metabolites. The information on genes involved in lignan biosynthesis is limited even in the model plant species Arabidopsis to date. We had established lignan profiling method and evaluated lignans in Arabidopsis. In this study, transcription activator-like effector nuclease (TALEN) was used to knock-out multiple UGT genes to reveal UGTs involved in glucosylation of lignans in Arabidopsis. It was revealed that UGT71C1 is able to transfer glucose from UDP-glucose to lignans, pinoresinol and lariciresinol in vitro. Less amount of lignan glucosides and more aglycones in mutants indicated that UGT71C1 is involved in lignan glucosylation in Arabidopsis.
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