| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
Collagen gels were irradiated by ultra violet (UV) light for intermolecularly-cross-linking. Young’s modulus of collagen gels increased after UV-irradiation. Collagen gels were irradiated with stripe pattern using photo-masking materials. Brain capillary endothelial cells (bEnd.3) were cultured on the UV-irradiated collagen gels. bEnd.3 cells were adhered on UV-irradiated collagen gel with stripe pattern. Next, mouse neural stem/progenitor cells formed neurosphere were seeded on UV-irradiated collagen gels with stripe patterned bEnd.3 cells. After the incubation with the differentiation medium for one week, stripe pattern consisted of bEnd.3 cells and differentiating cells form neural stem/progenitor cells were formed on UV-irradiated collagen gels.
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