Hi ghly sensitive electrochemical detection of influenza virus with nanosphere liposome encapsulating an inorganic complex

• Project/Area Number: 26420800
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Prefectural University of Hiroshima
• Principal Investigator: egashira naoyoshi 県立広島大学, 生命環境学部, 名誉教授 (90094060)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: バイオセンサ / リポソーム / インフルエンザウイルス / 電気化学検出 / フェロシアン化カリウム / 抗体 / 内包 / H1N1 / フェリシアン化カリウム

Outline of Final Research Achievements

We have developed a biosensor combining electrochemical detection with an immuliposome that encapsulates high concentration (0.4M) of potassium ferrocyanide. The liposome stably encapsulated the complex in over one month. Then we found that our method using a microplate enabled to detect BSA protein: interestingly the method does not need blocking procedure. Finally we successfully detected influenza virus of 1 x 104 PFU/mL within 20 min with our method that can be expected to be practically utilized.

Research Products

• [Journal Article] Detection of influenza virus by a biosensor based on the method combining electrochemiluminescence on binary SAMs modified Au electrode with an immunoliposome encapsulating Ru (II) complex (2016)
  • Author(s): Y. Katayama, T. Ohgi, Y. Mitoma, E. Hifumi, N. egashira
  • Journal Title: Anal Bioanal Chem Volume: 408 Issue: 22 Pages: 5963-5971
  • DOI: 10.1007/s00216-016-9587-8
  • Peer Reviewed
• [Presentation] フェロシアン化カリウム内包イムノリポソームと電気化学検出をくみあわせたインフルエンザウイルスの迅速定量 (2017)
  • Author(s): 中西莉子、臼井ありさ、三苫好治、一二三恵美、江頭直義
  • Organizer: 日本化学会第９７春季年会
  • Place of Presentation: 慶応義塾大学 日吉キャンパス
  • Year and Date: 2017-03-16
• [Presentation] フェロシアン化カリウム内包イムノリポソームを用いる電気化学検出によるタンパクの迅速定量 (2016)
  • Author(s): 中西莉子、稲毛咲矢香、三苫好治、江頭直義
  • Organizer: 日本化学会第９６春季年会
  • Place of Presentation: 同志社大学京田辺キャンパス
  • Year and Date: 2016-03-24
• [Presentation] Detection of influenza virus by an immunosensor combining liposome and electrochemiluminescence on Au electrode modified with binary self-assembled monolayers (2015)
  • Author(s): Katayama, Yumi; Mitoma,Yoshiharu; Hifumi, Emi; Egashira, Naoyoshi
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: ホノルル、ハワイ
  • Year and Date: 2015-12-17
  • Int'l Joint Research
• [Presentation] ２成分ＳＡＭ膜電極上におけるイムノリポソーム／電解発光によるインフルエンザウイルスの検出 (2015)
  • Author(s): 片山裕美
  • Organizer: 第９５回日本化学会春季年会
  • Place of Presentation: 日大理工（船橋キャンパス）
  • Year and Date: 2015-03-26
• [Patent(Industrial Property Rights)] 被検物質の定量方法 (2016)
  • Inventor(s): 江頭直義、三苫好治
  • Industrial Property Rights Holder: 公立大学法人県立広島大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2016-03-08