Hi ghly sensitive electrochemical detection of influenza virus with nanosphere liposome encapsulating an inorganic complex
| Project/Area Number |
26420800
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Prefectural University of Hiroshima |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | バイオセンサ / リポソーム / インフルエンザウイルス / 電気化学検出 / フェロシアン化カリウム / 抗体 / 内包 / H1N1 / フェリシアン化カリウム |
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| Outline of Final Research Achievements |
We have developed a biosensor combining electrochemical detection with an immuliposome that encapsulates high concentration (0.4M) of potassium ferrocyanide. The liposome stably encapsulated the complex in over one month. Then we found that our method using a microplate enabled to detect BSA protein: interestingly the method does not need blocking procedure. Finally we successfully detected influenza virus of 1 x 104 PFU/mL within 20 min with our method that can be expected to be practically utilized.
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Report
(4 results)
Research Products
(6 results)
