Construction of a library of oral vaccine on malaria using molecular display

• Project/Area Number: 26420803
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Hyogo University of Health Sciences
• Principal Investigator: SHIBASAKI Seiji 兵庫医療大学, 共通教育センター, 准教授 (50342530)
• Co-Investigator(Kenkyū-buntansha): 岩崎 剛 兵庫医療大学, 薬学部, 教授 (10151721)
• Research Collaborator: SANO Hajime 兵庫医科大学, 医学部, 教授 (00196304)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 分子ディスプレイ / 酵母 / ワクチン / ディスプレイ / 提示 / 抗原

Outline of Final Research Achievements

By using molecular display technology in yeast cells, a convenient method to develop oral vaccines such as malaria and other infectious diseases. A model proteins was efficiently displayed on the yeast cell surface. Kinds of host cells and anchoring protein were examined at first. Plasmids for creation of library , cultivate condition and preservative condition of yeast cell were also investigated.

Research Products

• [Journal Article] The Impact of MicroRNA-223-3p on IL-17 Receptor D Expression in Synovial Cells (2017)
  • Author(s): Nozomu Moriya, Seiji Shibasaki, Miki Karasaki, Tsuyoshi Iwasaki
  • Journal Title: PLoS ONE Volume: 12 Issue: 1 Pages: e0169702-e0169702
  • DOI: 10.1371/journal.pone.0169702
  • Peer Reviewed / Open Access
• [Journal Article] Oral Vaccine Development by Molecular Display Methods Using Microbial Cells (2016)
  • Author(s): Seiji Shibasaki, Mitsuyoshi Ueda
  • Journal Title: Methods Mol Biol. Volume: 1404 Pages: 497-509
  • DOI: 10.1007/978-1-4939-3389-1_32
  • ISBN: 9781493933884, 9781493933891
  • Peer Reviewed
• [Journal Article] Blocking c-Met signaling enhances bone morphogenetic protein-2-induced osteoblast differentiation. (2015)
  • Author(s): Shibasaki S, Kitano S, Karasaki M, Tsunemi S, Sano H, Iwasaki T.
  • Journal Title: FEBS Open Bio Volume: ５ Issue: 1 Pages: 341-347
  • DOI: 10.1016/j.fob.2015.04.008
  • Peer Reviewed / Open Access
• [Journal Article] Differential regulation of c-Met signaling pathways for synovial cell function. (2014)
  • Author(s): Shibasaki S, Tsunemi S, Kitano S, Sekiguchi M, Sano H, Iwasaki T.
  • Journal Title: SpringerPlus Volume: ３ Issue: 1 Pages: 554-554
  • DOI: 10.1186/2193-1801-3-554
  • Peer Reviewed / Open Access
• [Journal Article] Oral immunization against candidiasis using Lactobacillus casei displaying Enolase 1 from Candida albicans. (2014)
  • Author(s): Shibasaki S, Karasaki M, Tafuku S, Aoki W, Sewaki T, Ueda M.
  • Journal Title: Scientia Pharmaceutica Volume: 82 Issue: 3 Pages: 697-708
  • DOI: 10.3797/scipharm.1404-07
  • Peer Reviewed / Open Access
• [Journal Article] Combining proteomic strategies and molecular display technology for development of vaccines against Candida albicans. (2014)
  • Author(s): Shibasaki S, Karasaki M, Ueda M.
  • Journal Title: Journal of Proteomics & Bioinformatics Volume: 7 Issue: 06 Pages: 134-138
  • DOI: 10.4172/jpb.1000313
  • Peer Reviewed / Open Access
• [Journal Article] Evaluation of Mdh1 Protein as an Antigenic Candidate for a Vaccine against Candidiasis (2014)
  • Author(s): Shibasaki S, Aoki W, Nomura T, Karasaki M, Sewaki T, Ueda M.
  • Journal Title: Biocontrol Science Volume: 19 Issue: 1 Pages: 51-55
  • DOI: 10.4265/bio.19.51
  • NAID: 130003395463
  • ISSN: 1342-4815, 1884-0205
  • Peer Reviewed / Open Access
• [Presentation] Proteome analysis with a unique long monolithic capillary column of rheumatoid arthritis-related molecules in synovium (2016)
  • Author(s): S. Shibasaki, S. Aburaya, W. Aoki, M. Ueda, T. Iwasaki
  • Organizer: Biosensors 2016
  • Place of Presentation: Swedish Exhibition and Congress Center (Gothenburg, Sweden)
  • Year and Date: 2016-05-25
  • Int'l Joint Research
• [Presentation] NOVEL PLATFORM TECHNIQUE FOR PRODUCTION OF BIOPHARMACEUTICS USING YEAST CELL SURFACE DISPLAY SYSTEM (2014)
  • Author(s): Seiji Shibasaki
  • Organizer: 10th European Symposium on Biochemical Engineering Sciences
  • Place of Presentation: LILLE GRAND PALAIS (France)
  • Year and Date: 2014-09-08 – 2014-09-09