Studies on the evaluation method for chemical compound toxicity to human hepatocytes by using hollow fiber type three dimensional culture module
| Project/Area Number |
26420804
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Sojo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
石田 誠一 国立医薬品食品衛生研究所, 薬理部, 室長 (10270505)
古水 雄志 崇城大学, 生物生命学部, 助教 (80735829)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 三次元培養 / 胎児肝細胞 / 成人肝細胞 / 化学物質 / 毒性評価 / 亜急性毒性試験 / 動物実験代替法 / 安全性試験 |
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| Outline of Final Research Achievements |
The toxicity of about 30,000 chemical compounds on the earth should be evaluated by using in vitro cell-based assay system including three-dimensional (3D) culture module. In this research, (1) we have succeeded in the maintenance of the cell viability and CYP3A4 activity of the cryopreserved human hepatocytes for about one mont by perfusion culture using this 3D culture module. (2)We have developed the customized human hepatocyte DNA chip including 188 hepatic function related genes for rapid analysis of hepatic functions of the cells and confirmed the reproducibility of the analysis. (3)It was found by the DNA analysis and so on that the content and activity of mitochondria of fetal hepatocytes were lower than those of adult hepatocytes. And it was suggested that the toxicity differences of several chemical compounds between fetal and adult hepatocytes were depended on their mitochondrial contents and activities.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Comparative metabolome analysis of cultured fetal and adult hepatocytes in humans2014
Author(s)
S.-R. Kim, T. Kubo, Y. Kuroda, M. Hojyo, T. Matsuo, A. Miyajima, M. Usami, Y. Sekino, T. Matsushita, S. Ishida
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Journal Title
The Journal of Toxicological Sciences
Volume: 39
Issue: 5
Pages: 717-723
DOI
NAID
ISSN
0388-1350, 1880-3989
Related Report
Peer Reviewed / Open Access
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[Journal Article] Inflammatory cytokines promote the retrodifferentiation of tumor-derived hepatocyte-like cells to progenitor cells2014
Author(s)
H. Dubois-Pot-Schneider, K. Fekir, C. Coulouarn,D. Glaise, C. Aninat, K. Jarnouen, R. Le Guevel, T. Kubo, S. Ishida, F. Morel, A. Corlu
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Journal Title
Hepatology
Volume: 60
Issue: 6
Pages: 2077-2090
DOI
Related Report
Peer Reviewed
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