| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
The toxicity of about 30,000 chemical compounds on the earth should be evaluated by using in vitro cell-based assay system including three-dimensional (3D) culture module. In this research, (1) we have succeeded in the maintenance of the cell viability and CYP3A4 activity of the cryopreserved human hepatocytes for about one mont by perfusion culture using this 3D culture module. (2)We have developed the customized human hepatocyte DNA chip including 188 hepatic function related genes for rapid analysis of hepatic functions of the cells and confirmed the reproducibility of the analysis. (3)It was found by the DNA analysis and so on that the content and activity of mitochondria of fetal hepatocytes were lower than those of adult hepatocytes. And it was suggested that the toxicity differences of several chemical compounds between fetal and adult hepatocytes were depended on their mitochondrial contents and activities.
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