| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
To determine the essential region working as a promoter, I constructed expression vectors including a secreted leucine aminopeptidase gene from Streptomyces sp. under the SCMP promotor, which was identified a powerful promoter from S. cinnamoneus TH-2. The vectors were containing of the first half, the last half, 63 bps from the 3’ termini and 43 bps from the 3’ termini of the SCMP promoter, respectively. By using S. lividans 1326 and above vectors, secreted aminopeptidase activities were compared. From the results, there is no working by using the first half of SCMP promoter. On the other hand, 63 bps from the 3’ termini showed a strong activity. Moreover, it is possible to use various expression media when the region was used as a promotor.
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