| Project/Area Number |
26430005
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | National Center of Neurology and Psychiatry (2016)
The University of Tokyo (2014-2015) |
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Principal Investigator |
NOGUCHI Jun 国立研究開発法人国立精神・神経医療研究センター, 神経研究所 微細構造研究部, 室長 (40421367)
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| Co-Investigator(Renkei-kenkyūsha) |
KASAI Haruo 東京大学, 大学院医学系研究科, 教授 (60224375)
TAKAHASHI Noriko 東京大学, 大学院医学系研究科, 講師 (60332178)
WATANABE Satoshi 東京大学, 大学院医学系研究科, 助教 (80302610)
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| Research Collaborator |
UCAR Hasan 東京大学, 大学院医学系研究科, 博士研究員
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 樹状突起スパイン / プレシナプスブトン / 逆行性シグナル / 機械的刺激 / シナプス / シナプス可塑性 / スパイン形態可塑性 / 逆行性情報伝達 / 蛍光寿命画像顕微鏡 / FRET / ２光子顕微鏡 / プレシナプス / 2光子顕微鏡 |
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| Outline of Final Research Achievements |
We investigated whether the synaptic plasticity information can be transmitted from post-synaptic to pre-synaptic sites by mechanistic stimulation of presynaptic membrane. First, we developed a fluorescent probe (iSLIM) which can quantitate presynaptic function using FRET method. Then we examined it in rat hippocampal slice CA1 area. We could detect accumulations of SNARE complexes which are the indicator of the presynaptic function in Schaffer collateral synaptic boutons using this probe.
Next, we evaluated the difference of presynaptic function after mechanistic stimulations such as high-osmotic pressure, using this functional probe. The results showed that the mechanistic stimulations for the presynaptic site cause an increase of presynaptic function.
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