Analysis of the properties of synaptic back propagating signal

• Project/Area Number: 26430005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: National Center of Neurology and Psychiatry (2016); The University of Tokyo (2014-2015)
• Principal Investigator: NOGUCHI Jun 国立研究開発法人国立精神・神経医療研究センター, 神経研究所 微細構造研究部, 室長 (40421367)
• Co-Investigator(Renkei-kenkyūsha): KASAI Haruo 東京大学, 大学院医学系研究科, 教授 (60224375) ; TAKAHASHI Noriko 東京大学, 大学院医学系研究科, 講師 (60332178) ; WATANABE Satoshi 東京大学, 大学院医学系研究科, 助教 (80302610)
• Research Collaborator: UCAR Hasan 東京大学, 大学院医学系研究科, 博士研究員
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 樹状突起スパイン / プレシナプスブトン / 逆行性シグナル / 機械的刺激 / シナプス / シナプス可塑性 / スパイン形態可塑性 / 逆行性情報伝達 / 蛍光寿命画像顕微鏡 / FRET / ２光子顕微鏡 / プレシナプス / 2光子顕微鏡

Outline of Final Research Achievements

We investigated whether the synaptic plasticity information can be transmitted from post-synaptic to pre-synaptic sites by mechanistic stimulation of presynaptic membrane. First, we developed a fluorescent probe (iSLIM) which can quantitate presynaptic function using FRET method. Then we examined it in rat hippocampal slice CA1 area. We could detect accumulations of SNARE complexes which are the indicator of the presynaptic function in Schaffer collateral synaptic boutons using this probe.
Next, we evaluated the difference of presynaptic function after mechanistic stimulations such as high-osmotic pressure, using this functional probe. The results showed that the mechanistic stimulations for the presynaptic site cause an increase of presynaptic function.

Research Products

• [Journal Article] State-dependent diffusion of actin-depolymerizing factor/cofilin underlies the enlargement and shrinkage of dendritic spines. (2016)
  • Author(s): Noguchi, J., Hayama, T., Watanabe, S., Ucar, H., Yagishita, S., Takahashi, N. & Kasai, H.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 32897-32897
  • DOI: 10.1038/srep32897
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Implantable Microfluidic Device with Hydrogel Permeable Membrane for Delivering Chemical Compounds and Imaging Neural Cells in Living Mice (2016)
  • Author(s): Takehara, H., Nagaoka, A., Noguchi, J., Akagi, T., Kasai, H. & Ichiki, T.
  • Journal Title: Journal of Photopolymer Science and Technology Volume: 29 Issue: 4 Pages: 513-518
  • DOI: 10.2494/photopolymer.29.513
  • NAID: 130005261780
  • ISSN: 0914-9244, 1349-6336
  • Peer Reviewed / Open Access
• [Journal Article] Abnormal intrinsic dynamics of dendritic spines in a fragile X syndrome mouse model in vivo. (2016)
  • Author(s): Nagaoka A, Takehara H, _Hayashi-Takagi A_, Noguchi J, Ishii K, Shirai F, Yagishita S, Akagi T, Ichiki T, Kasai H.
  • Journal Title: Scientific Reports Volume: 6 Issue: 1 Pages: 26651-26651
  • DOI: 10.1038/srep26651
  • Peer Reviewed / Open Access
• [Journal Article] Two-photon fluorescence lifetime imaging of primed SNARE complexes in presynaptic terminals and β cells. (2015)
  • Author(s): Takahashi N#, Sawada W#, Noguchi J#, Watanabe S#, Ucar H, Hayashi-Takagi A, Yagishita S, Ohno M, Tokumaru H & Kasai H. (#Equally contributed).
  • Journal Title: Nature Communications Volume: 6 Issue: 1 Pages: 8531-8531
  • DOI: 10.1038/ncomms9531
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Lab-on-a-brain : Implantable micro-optical fluidic devices for neural cell analysis in vivo (2014)
  • Author(s): Takehara, H.*, Nagaoka, A.*, Noguchi, J., Akagi, T., Kasai, H. & Ichiki, T.
  • Journal Title: Scientific Report Volume: 4 Issue: 1 Pages: 6721-6721
  • DOI: 10.1038/srep06721
  • Peer Reviewed
• [Journal Article] Caged Glutamates with π-Extended 1, 2-Dihydronaphthalene Chromophore : Design, Synthesis, Two-Photon Absorption Property, and Photochemical Reactivity (2014)
  • Author(s): Boinapally, S., Huang, B., Abe, M., Katan, C., Noguchi, J., Watanabe, S., Kasai, H., Xue, B. & Kobayashi, T.
  • Journal Title: Opt Express Volume: 22 Issue: 17 Pages: 7822-30
  • DOI: 10.1021/jo501425p
  • Peer Reviewed
• [Presentation] 2光子励起蛍光寿命画像法を用いたSNARE複合化の解析 (2016)
  • Author(s): 高橋 倫子、澤田 和可子、野口 潤、ハサン ウチャル、渡辺 惠、河西 春郎
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-07-22
  • Invited
• [Presentation] FRET-FLIM measurement with a trans-SNARE-probe detecting the mechanical force from dendritic spine to pre-synaptic bouton. (2016)
  • Author(s): ハサン ウチャル、渡辺 惠、野口 潤、高橋 倫子、澤田 和可子、栁下 祥、河西 春郎
  • Organizer: 第39回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-07-22
• [Presentation] シナプス可塑性記録のためのプレシナプスプローブの海馬培養スライスにおける使用 (2014)
  • Author(s): 野口潤、渡邊恵、高橋倫子、河西春郎
  • Organizer: 日本神経科学学会
  • Place of Presentation: パシフィコ横浜（神奈川県、横浜市）
  • Year and Date: 2014-09-11
• [Book] 脳内環境辞典 （分担執筆） (2017)
  • Author(s): 高橋良輔、山中宏二、樋口真人、漆谷真（編）
  • Total Pages: 156
  • Publisher: メディカルドゥ
• [Remarks] 東京大学大学院医学系研究科 構造生理学部門
• [Remarks] 東京大学大学院医学系研究科疾患生命工学センター構造生理学部門ホームページ
  • URL: http://www.bm2.m.u-tokyo.ac.jp/