Target analyses of Otx2 homeoprotein in postnatal plasticity

• Project/Area Number: 26430010
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Niigata University
• Principal Investigator: SUGIYAMA Sayaka 新潟大学, 医歯学系, 准教授 (10360570)
• Research Collaborator: SAKAI Akiko
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 臨界期 / ホメオ蛋白質 / 抑制性介在ニューロン / 眼優位性可塑性 / 視覚野 / 眼優位性 / Otx2 / 弱視

Outline of Final Research Achievements

Neuronal circuits are sharpened by experience during critical periods in early life. We previously demonstrated that Otx2 functions as an inducer of the critical period in mouse postnatal visual cortex. In this study, we investigated Otx2 target genes such as transcription factors or homeoproteins by chromatin immunoprecipitation sequencing (ChIP-seq) and cell-type specific RNA-seq analyses for critical period plasticity. Our results suggest some homeoproteins and immediate-early genes (IEGs) as novel targets of Otx2. Otx2 may render the chromatin environment capable of an immediate response to neuronal depolarization and may be required for such a ‘poised’ state of histone modifications of IEGs promoters. Our data indicates that Otx2-induced genetic cascade is broadly involved in neuronal plasticity as well as critical period.

Research Products

• [Journal Article] Chondroitin Sulfate Is Required for Onset and Offset of Critical Period Plasticity in Visual Cortex (2017)
  • Author(s): Xubin Hou, Nozomu Yoshioka, Hiroaki Tsukano, Akiko Sakai, Shinji Miyata, Yumi Watanabe, Yuchio Yanagawa, Kenji Sakimura, Kosei Takeuchi, Hiroshi Kitagawa, Takao Hensch, Katsuei Shibuki, Michihiro Igarashi, Sayaka Sugiyama
  • Journal Title: Scientific Reports Volume: ―
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genome-Wide Target Analyses of Otx2 Homeoprotein in Postnatal Cortex (2017)
  • Author(s): Akiko Sakai, Ryuichiro Nakato, Yiwei Ling, Xubin Hou, Norikazu Hara, Tomoya Iijima, Yuchio Yanagawa, Ryozo Kuwano, Shujiro Okuda, Katsuhiko Shirahige, Sayaka Sugiyama
  • Journal Title: Frontiers in Neuroscience Volume: ―
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 臨界期の機構 ― こどもの脳の柔軟性を紐解く (2017)
  • Author(s): 酒井晶子、杉山清佳
  • Journal Title: 生体の科学 Volume: 68 Pages: 54-58
  • Peer Reviewed
• [Presentation] Transcriptional regulation of homeoprotein in postnatal cortical plasticity (2017)
  • Author(s): Sugiyama S
  • Organizer: The 40th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 幕張メッセ（千葉県千葉市）
  • Year and Date: 2017-07-20
  • Invited
• [Presentation] Identification of Otx2-eIF4E targetome in cortical plasticity with RNA immunoprecipitation-sequencing (2016)
  • Author(s): Hou X, Ling Y, Sakai A, Okuda S and Sugiyama S
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
• [Presentation] Experience-dependent maturation of inhibitory circuits in ocular dominance plasticity (2016)
  • Author(s): Sugiyama S and Hou X
  • Organizer: The 39th Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-07-20
  • Invited
• [Presentation] コンドロイチン硫酸による量依存的な臨界期制御 (2015)
  • Author(s): 杉山清佳
  • Organizer: 分子生物学会
  • Place of Presentation: 神戸ポートアイランド（兵庫県・神戸市）
  • Year and Date: 2015-12-01
  • Invited
• [Presentation] Ocular dominance plasticity regulated by Otx2-inducible molecules (2015)
  • Author(s): Sayaka Sugiyama
  • Organizer: 神経化学会
  • Place of Presentation: 大宮ソニックシティ（埼玉県・大宮市）
  • Year and Date: 2015-09-11
  • Invited
• [Presentation] Coactosin, an actin-remodeling factor promotes maturation of parvalbumin-positive cells for cortical plasticity (2015)
  • Author(s): Xubin Hou, Kenji Sakimura, Sayaka Sugiyama
  • Organizer: 神経科学会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Involvement of Otx2 homeoprotein in regulating activity-dependent genes to activate plasticity (2015)
  • Author(s): Akiko Sakai, Ryuichiro Nakato, Tomoya Iijima, Norikazu Hara, Yuchio Yanagawa, Ryozo Kuwano, Katsuhiko Shirahige, Sayaka Sugiyama
  • Organizer: 神経科学会
  • Place of Presentation: 神戸コンベンションセンター（兵庫県・神戸市）
  • Year and Date: 2015-07-28
• [Presentation] Inhibitory maturation regulates the critical period plasticity for binocular vision (2015)
  • Author(s): Sayaka Sugiyama
  • Organizer: 第１２０回日本解剖学会 第９２回日本生理学会大会 合同大会
  • Place of Presentation: 神戸市（神戸国際会議場）
  • Year and Date: 2015-03-21 – 2015-03-23
  • Invited
• [Presentation] Identification of plastic genes by transcriptome and ChIP-seq of Otx2 homeoprotein (2014)
  • Author(s): Akiko Sakai, Ryuichiro Nakato, Norikazu Hara, Yuchio Yanagawa, Ryozo Kuwano, Katsuhiko Shirahige, Sayaka Sugiyama
  • Organizer: 第３７回日本神経科学大会
  • Place of Presentation: 横浜市（パシフィコ横浜）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] 臨界期の時期を制御する分子たち (2014)
  • Author(s): 杉山清佳
  • Organizer: 第18回視覚科学フォーラム
  • Place of Presentation: 前橋市（前橋工科大学）
  • Year and Date: 2014-08-18 – 2014-08-19
  • Invited
• [Book] 脳科学辞典 項目名：臨界期 (2015)
  • Author(s): 杉山清佳
  • Publisher: 脳科学辞典編集委員会（日本神経科学会）
• [Book] 分子脳科学 (2015)
  • Author(s): 杉山清佳
  • Total Pages: 291
  • Publisher: 化学同人