| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
Olfaction starts from the olfactory cilia with nano-scale structure. It is difficult to apply to cell experiment because It involves distribution between air/water phases of molecules and molecular chain reaction within the cilia as nano-scale structure. In addition, many questions remain and we couldn't elucidate that system quantitatively. In this research, we developed new methods and used it for making clear the transduction system within the nano-scale structure cilia. For that, we needed to refine conventional patch clamp technique, nano-imaging technique, laser beams and combined photolysis of caged substances within tiny space. Furthermore, we combined a low noise current analysis of with high S/N ratio into new system. As a result, we could monitor its molecular dynamics in real time in the tiny living tissue and clarified the modification mechanism of olfactory transduction system using channels. We made a paper regarding this result and presented in the conference.
|
