Modulation mechanism of transduction channels in the olfactory cilia
| Project/Area Number |
26430016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 情報伝達機構 / イオンチャネル / ナノスケール / 線毛 / パッチクランプ法 / 電流 / 嗅覚 / ナノバイオロジー / 電気生理学 / 嗅細胞 / 飲食品 |
|---|
| Outline of Final Research Achievements |
Olfaction starts from the olfactory cilia with nano-scale structure. It is difficult to apply to cell experiment because It involves distribution between air/water phases of molecules and molecular chain reaction within the cilia as nano-scale structure. In addition, many questions remain and we couldn't elucidate that system quantitatively. In this research, we developed new methods and used it for making clear the transduction system within the nano-scale structure cilia. For that, we needed to refine conventional patch clamp technique, nano-imaging technique, laser beams and combined photolysis of caged substances within tiny space. Furthermore, we combined a low noise current analysis of with high S/N ratio into new system. As a result, we could monitor its molecular dynamics in real time in the tiny living tissue and clarified the modification mechanism of olfactory transduction system using channels. We made a paper regarding this result and presented in the conference.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果により、感覚細胞における情報変換の分子機構を定量的に説明し、生体情報入力に関する分子動態的知見をもたらすことで、今後、ニューロンを含む生体細胞の微細構造部分（各種線毛、細胞小器官、デンドライトや軸索、シナプス、分泌顆粒）での分子機能研究に応用される進展が期待される。
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Report
(6 results)
Research Products
(27 results)
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[Book] Taste and Smell2017
Author(s)
Hiroko Takeuchi, Takashi Kurahashi, Dietmar Krautwurst(Ed)
Total Pages
199
Publisher
Springer International Publishing
ISBN
9783319489254
Related Report
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[Book] Topics in Medical Chemistry, Taste and Small2017
Author(s)
Takashi Kurahashi, Hiroko Takeuchi, Maik Behrens, Simone Prandi, Wolfgang Meyerhof, Joshua Fletcher, Li Pan, Douglas Kinghon, Brett Boonen, Justyna Startek, Karel Talavera, Patrick Marcinek, Christiane Geithe, Stefano Espinoza, Raul Gainetdinov, Dietmar Krautwurst (Ed)
Total Pages
199
Publisher
Springer
Related Report
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