Development and evolution of the frontal cortex

• Project/Area Number: 26430033
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Tohoku University
• Principal Investigator: Sato Tatsuya 東北大学, 情報科学研究科, 准教授 (00568222)
• Co-Investigator(Kenkyū-buntansha): 大隅 典子 東北大学, 医学系研究科, 教授 (00220343)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 内側前頭前野 / 嗅球 / 眼窩前頭皮質 / 電気穿孔法 / マウス / 線維芽細胞増殖因子 / 形態形成 / FGF8 / 大脳皮質 / 前頭前野 / 神経回路形成 / ドーパミン / 錐体細胞 / 樹状突起

Outline of Final Research Achievements

The frontal cortex (FC) is an important brain region for motor function as well as decision making and social or cognitive behavior. Here we show the organizing molecule, fibroblast growth factor 8 (Fgf8), has an effect to medialize the dorsal part of the FC. We analyzed expression of FC subdivision markers in Fgf8-transfected mouse brains. When Fgf8 was misexpressed in the anterior pole of the telencephalon by in vivo electroporation, the dorsomedial part of the FC (anterior cingulate and prelimbic cortex) was expanded. Expression borders of dorsal FC subdivision markers shifted laterally. We also found that the morphology of the Fgf8-transfected brains was quite abnormal: the ventral FC (the orbital cortex) was not formed and the olfactory bulb was larger than that of wild-type brains. These findings suggest that Fgf8 is involved in the formation of FC subdivision and olfactory bulb development.

Research Products

• [Journal Article] Targeted disruption of the mouse protein phosphatase ppm1l gene leads to structural abnormalities in the brain. (2016)
  • Author(s): Kusano, R., Kousuke Fujita, K., Yasuharu Shinoda, Y., Nagaura, Y., Kiyonari, H., Abe, T, Watanabe, T., Matsui, Y., Fukaya, M., Sakagami, H., Sato, T., Funahashi, J.-I., Ohnishi, M., Tamura, S., Kobayashi, T.
  • Journal Title: FEBS Letters Volume: 590 Issue: 20 Pages: 3603-3615
  • DOI: 10.1002/1873-3468.12429
  • Peer Reviewed
• [Journal Article] Fgf8 signaling for development of the midbrain and hindbrain. (2016)
  • Author(s): Harada, H., Sato, T. & Nakamura, H.
  • Journal Title: Dev. Growth Differ. Volume: 58 Issue: 5 Pages: 473-445
  • DOI: 10.1111/dgd.12293
  • Peer Reviewed
• [Journal Article] Nepro is localized in the nucleolus and essential for preimplantation development in mice. (2015)
  • Author(s): Masakazu Hashimoto, Tatsuya Sato, Yuko Muroyama, Lisa Fujimura, Masahiko Hatano, Tetsuichiro Saito
  • Journal Title: Development, Growth & Differentiation Volume: 57 Issue: 7 Pages: 529-538
  • DOI: 10.1111/dgd.12232
  • Peer Reviewed
• [Journal Article] Pea3 determines the isthmus region at the downstream of Fgf8-Ras-ERK signaling pathway (2015)
  • Author(s): Harada, H., Omi, M., Sato, T. & Nakamura, H.
  • Journal Title: Dev. Growth Differ. Volume: 57 Issue: 9 Pages: 657-666
  • DOI: 10.1111/dgd.12254
  • Peer Reviewed
• [Presentation] Role of Fgf8 for regionalization of the frontal cortex (2017)
  • Author(s): Sato, T., Kuwata, R., Nakamura, H., Itoi, K. & Osumi, N.
  • Organizer: 第10回神経発生討論会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-10
• [Presentation] Sex differences of CRF-expressing neurons in the bed nucleus of the stria terminalis of the mouse (2017)
  • Author(s): Hiroko Otsuka, Katsuya Uchida, Masahiro Morisita, Shinji Tsukahara, Toshimitsu Fuse, Yuto Kagotani, Satoshi Yamagata, Tatsuya Sato, Manabu Abe, Kenji Sakimura, Keiichi Itoi
  • Organizer: The 2017 Japan-NIH joint Symposium on Advances in Biomedical Research and Disease
  • Place of Presentation: 仙台
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] Generation and application of CRF neuron-selective channelrhodopsin-2 expressing mouse (2017)
  • Author(s): Satoshi Yamagata, Isumi Akiba, Tatsuya Sato, Katsuya Uchida, Li Zhou, Rie Natsume, Manabu Abe, Kenji Sakimura, Keiichi Itoi
  • Organizer: The 2017 Japan-NIH joint Symposium on Advances in Biomedical Research and Disease
  • Place of Presentation: 仙台
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] Identification of homeobox genes regulating expression of catecholamine-synthesizing enzymes in the locus ceruleus (2017)
  • Author(s): Tatsuya Sato, Katsuya Uchida, Yasumasa Iwasaki, Satoshi Yamagata, Chiaki Tanaka, Hiroaki Tomita, Keiichi Itoi
  • Organizer: The 2017 Japan-NIH joint Symposium on Advances in Biomedical Research and Disease
  • Place of Presentation: 仙台
  • Year and Date: 2017-02-16
  • Int'l Joint Research
• [Presentation] Dendtritic morphology of the cortical pyramidal neurons is regulated by dopaminergic signal in early postnatal mice (2015)
  • Author(s): Sato, T., Saito, T. & Osumi, N.
  • Organizer: Tohoku Forum for Creativity, Memory and Mind
  • Place of Presentation: 仙台
  • Year and Date: 2015-09-29
• [Presentation] Dendtritic morphology of the cortical pyramidal neurons is regulated by dopaminergic signal in early postnatal mice (2015)
  • Author(s): Sato, T., Saito, T. & Osumi, N.
  • Organizer: Tohoku Forum for Creativity, Development and Disease
  • Place of Presentation: 仙台
  • Year and Date: 2015-08-25
• [Presentation] Dendtritic morphology of the cortical pyramidal neurons is regulated by dopaminergic signal in early postnatal mice (2015)
  • Author(s): Sato, T., Saito, T. & Osumi, N.
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-07-28
• [Presentation] Postmitotic dorsal spinal cord neurons are transfated into commissural neurons by induced misexpression of Barh1 (2014)
  • Author(s): Sato, T., Muroyama, Y. & Saito, T.
  • Organizer: 第44回北米神経科学会
  • Place of Presentation: アメリカ合衆国、ワシントン
  • Year and Date: 2014-11-18
• [Book] 脳科学辞典「エンハンサー」 (2015)
  • Author(s): 佐藤達也、齋藤哲一郎
  • Total Pages: 1
  • Publisher: 理化学研究所
• [Book] NEUROMETHODS "Electroporation Methods and Neuroscience" Chapter 15 (2015)
  • Author(s): Sato, T., Muroyama, Y. and Saito, T.
  • Total Pages: 9
  • Publisher: Springer
• [Book] Dojin Bioscience シリーズ「分子脳科学」第20章 (2015)
  • Author(s): 佐藤達也、大隅典子
  • Total Pages: 13
  • Publisher: 化学同人
• [Remarks] 研究概要
  • URL: http://www.fris.tohoku.ac.jp/fris/organization/creative/satoTatsuya.html
• [Remarks] 「精神」「知性」とは？
  • URL: http://www.dev-neurobio.med.tohoku.ac.jp/researchoutline/osumi/intellect/index.html