Analysis of signaling for axon formation in corticospinal tract development
| Project/Area Number |
26430036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Fujita Health University |
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Principal Investigator |
Omi Minoru 藤田保健衛生大学, 医学部, 助教 (00400416)
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 真 大阪大学, 連合小児発達学研究科, 教授 (10222019)
岡 雄一郎 大阪大学, 連合小児発達学研究科, 講師 (30614432)
猪口 徳一 大阪大学, 医学系研究科, 助教 (60509305)
黒田 一樹 福井大学, 学術研究院医学系部門, 助教 (60557966)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 神経科学 / 神経細胞 / 細胞分化 / 神経回路 / 解剖学 / 発生・発達 |
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| Outline of Final Research Achievements |
Pyramidal neurons in the cortex form corticospinal tract that project to the spinal cord, generating some collaterals projecting to some nuclei such as pontine nucleus. To identify signaling molecules that function during the corticospinal tract development, we performed screening of genes of receptor molecules that may be expressed in the pyramidal neurons by knockdown experiment with shRNA expressing vectors. We focused two of genes listed by the screening which showed loss or truncation of collaterals in the pons when each was knocked down. We confirmed that those were expressed in the fifth layer in the cortex of newborns. When cortical neurons were cultured in the media with ligand protein of the receptor, blanching of the neurites was increased, suggesting that they may be involved in collateral formation of the pyramidal tract.
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Report
(4 results)
Research Products
(11 results)
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[Journal Article] DBZ regulates cortical cell positioning and neurite development by sustaining the anterograde transport of Lis1 and DISC1 through control of Ndel1 dual-phosphorylation.2015
Author(s)
1.Okamoto, M., Iguchi, T., Hattori, T., Matsuzaki, S., Koyama, Y., Taniguchi, M., Kodama, M., Xie, M-J., Yagi, H., Shimizu, S., Konishi, Y., Yoshimi, T., Tachibana, T., Fujieda, S., Katayama, T., Ito, A., Hirotsune, S., Tohyaman, M. and Sato, M.
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Journal Title
J Neurosci
Volume: 18
Issue: 7
Pages: 2942-2958
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] DISC1-binding zinc finger protein (DBZ) regulates cortical cell positioning and neurite elongation through control of Ndel1 dual-phosphorylation2015
Author(s)
Iguchi T, Okamoto M, Hattori T, Matsuzaki S, Koyama Y, Taniguchi M, Komada M, Xie M, Yagi H, Shimizu S, Omi M, Katayama T, Ito A, Hirotsune S, Tohyama M, Sato M
Organizer
Neuroscience 2015, SfN's 45th annual meeting
Place of Presentation
米国、シカゴ
Year and Date
2015-10-17
Related Report
Int'l Joint Research
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