Analysis of signaling for axon formation in corticospinal tract development

• Project/Area Number: 26430036
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Fujita Health University
• Principal Investigator: Omi Minoru 藤田保健衛生大学, 医学部, 助教 (00400416)
• Co-Investigator(Kenkyū-buntansha): 佐藤 真 大阪大学, 連合小児発達学研究科, 教授 (10222019) ; 岡 雄一郎 大阪大学, 連合小児発達学研究科, 講師 (30614432) ; 猪口 徳一 大阪大学, 医学系研究科, 助教 (60509305) ; 黒田 一樹 福井大学, 学術研究院医学系部門, 助教 (60557966)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 神経科学 / 神経細胞 / 細胞分化 / 神経回路 / 解剖学 / 発生・発達

Outline of Final Research Achievements

Pyramidal neurons in the cortex form corticospinal tract that project to the spinal cord, generating some collaterals projecting to some nuclei such as pontine nucleus. To identify signaling molecules that function during the corticospinal tract development, we performed screening of genes of receptor molecules that may be expressed in the pyramidal neurons by knockdown experiment with shRNA expressing vectors. We focused two of genes listed by the screening which showed loss or truncation of collaterals in the pons when each was knocked down. We confirmed that those were expressed in the fifth layer in the cortex of newborns. When cortical neurons were cultured in the media with ligand protein of the receptor, blanching of the neurites was increased, suggesting that they may be involved in collateral formation of the pyramidal tract.

Research Products

• [Journal Article] Pea3 determines the isthmus region at the downstream of Fgf8-Ras-ERK signaling pathway (2015)
  • Author(s): Harada, H., Omi, M., Sato, T. & Nakamura, H.
  • Journal Title: Dev. Growth Differ. Volume: 57 Issue: 9 Pages: 657-666
  • DOI: 10.1111/dgd.12254
  • Peer Reviewed
• [Journal Article] DBZ regulates cortical cell positioning and neurite development by sustaining the anterograde transport of Lis1 and DISC1 through control of Ndel1 dual-phosphorylation. (2015)
  • Author(s): 1.Okamoto, M., Iguchi, T., Hattori, T., Matsuzaki, S., Koyama, Y., Taniguchi, M., Kodama, M., Xie, M-J., Yagi, H., Shimizu, S., Konishi, Y., Yoshimi, T., Tachibana, T., Fujieda, S., Katayama, T., Ito, A., Hirotsune, S., Tohyaman, M. and Sato, M.
  • Journal Title: J Neurosci Volume: 18 Issue: 7 Pages: 2942-2958
  • DOI: 10.1523/jneurosci.5029-13.2015
  • Peer Reviewed / Open Access
• [Journal Article] Engrailed and tectum development (2015)
  • Author(s): Omi M, Nakamura H
  • Journal Title: Development, Growth & Differentiation Volume: 57(2) Issue: 2 Pages: 135-145
  • DOI: 10.1111/dgd.12197
  • Peer Reviewed
• [Journal Article] Role of En2 in the tectal laminar formation of chick embryos (2014)
  • Author(s): Omi M., Harada H., Watanabe Y., Funahashi J., Nakamura H.
  • Journal Title: Development Volume: 141(10) Issue: 10 Pages: 2131-2138
  • DOI: 10.1242/dev.102905
  • Peer Reviewed
• [Presentation] DISC1-binding zinc finger protein (DBZ) regulates cortical cell positioning and neurite elongation through control of Ndel1 dual-phosphorylation (2015)
  • Author(s): Iguchi T, Okamoto M, Hattori T, Matsuzaki S, Koyama Y, Taniguchi M, Komada M, Xie M, Yagi H, Shimizu S, Omi M, Katayama T, Ito A, Hirotsune S, Tohyama M, Sato M
  • Organizer: Neuroscience 2015, SfN's 45th annual meeting
  • Place of Presentation: 米国、シカゴ
  • Year and Date: 2015-10-17
  • Int'l Joint Research
• [Presentation] 皮質脊髄路側枝形成を制御する受容体による皮質-橋-小脳路の形成機構 (2015)
  • Author(s): 猪口徳一、尾身 実、岡 雄一郎、佐藤 真
  • Organizer: 第16回 ORIGIN 神経科学研究会 2015
  • Place of Presentation: 下関市豊北生涯学習センター、山口県下関市
  • Year and Date: 2015-08-28
• [Presentation] 受容体チロシンキナーゼとその反発性結合分子は脳内で相補的に発現することで軸索側枝の投射様式を制御し皮質‐橋‐小脳路の形成に関わる (2015)
  • Author(s): 猪口徳一、尾身 実、岡 雄一郎、佐藤 真
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場・展示場、兵庫県神戸市
  • Year and Date: 2015-07-28
• [Presentation] 海馬神経細胞におけるNMDAレセプタに結合するFILIP関連分子の機能解析 (2015)
  • Author(s): 黒田一樹、八木秀司、謝 敏かく、尾身 実、深澤 有吾、岡 雄一郎、猪口 徳一、佐藤 真
  • Organizer: 第38回日本神経科学大会
  • Place of Presentation: 神戸国際会議場・展示場、兵庫県神戸市
  • Year and Date: 2015-07-28
• [Presentation] Analysis on the candidate receptors for the axon collateralization of the developing corticospinal tract (2015)
  • Author(s): Iguchi T, Omi M, Oka Y, Sato M
  • Organizer: 第120回 日本解剖学会総会全国学術集会
  • Place of Presentation: 兵庫県神戸市・神戸国際会議場
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 皮質脊髄路側枝形成に関与する受容体分子の探索と解析 (2014)
  • Author(s): 猪口徳一、尾身実、岡雄一郞、森泰丈、佐藤真
  • Organizer: 第90回 日本解剖学会近畿支部学術集会
  • Place of Presentation: 大阪府吹田市・大阪大学医学部
  • Year and Date: 2014-11-29
• [Book] Avian and Reptilian Developmental Biology (Methods in Molecular Biology, vol.1650) (2017)
  • Author(s): Harada H., Omi M., Nakamura H. (Ed. Sheng G.)
  • Total Pages: 390
  • Publisher: Humana Press