| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Outline of Final Research Achievements |
We analyzed developmental establishment of the cell-lineage-dependent neural connections in mouse barrel cortex using chimeric mice which is generated by injection of GFP-expressing mouse-derived iPS cells.
There was no significant difference in the connection probability between clonal and non-clonal pairs at P9-11. The probability increased significantly from P9-11 to P14-16, then decreased only in clonal pairs, resulting in the same connection probability in clonal and non-clonal pairs at P18-20. The proportion of reciprocally connected pairs among connected pairs was not significantly different between clonal and non-clonal neuron pairs at P9-11. Then the reciprocity continued to increase significantly in clonal pairs until P18-20, whereas it showed only an insignificant increase in non-clonal pairs during that period. These results suggest that cell lineage dependent reciprocal connections are formed accompanied with cell lineage specific reconstruction of synaptic connectivity.
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