To reveal the mechanisms of cell-lineage dependent coritcal neural circuits formation during postnatal development
Project/Area Number |
26430047
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Nerve anatomy/Neuropathology
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Research Institution | Osaka University (2017) National Institute for Physiological Sciences (2014-2016) |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | 神経回路 / シナプス結合 / synapse / 微小神経回路 |
Outline of Final Research Achievements |
We analyzed developmental establishment of the cell-lineage-dependent neural connections in mouse barrel cortex using chimeric mice which is generated by injection of GFP-expressing mouse-derived iPS cells. There was no significant difference in the connection probability between clonal and non-clonal pairs at P9-11. The probability increased significantly from P9-11 to P14-16, then decreased only in clonal pairs, resulting in the same connection probability in clonal and non-clonal pairs at P18-20. The proportion of reciprocally connected pairs among connected pairs was not significantly different between clonal and non-clonal neuron pairs at P9-11. Then the reciprocity continued to increase significantly in clonal pairs until P18-20, whereas it showed only an insignificant increase in non-clonal pairs during that period. These results suggest that cell lineage dependent reciprocal connections are formed accompanied with cell lineage specific reconstruction of synaptic connectivity.
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Report
(5 results)
Research Products
(10 results)
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[Journal Article] 1.Establishment of high reciprocal connectivity between clonal cortical neurons is regulated by the Dnmt3b DNA methyltransferase and clustered protocadherins.2016
Author(s)
Tarusawa E, Sanbo M, Okayama A, Miyashita T, Kitsukawa T, Hirayama T, Hirabayashi T, Hasegawa S, Kaneko R, Toyoda S, Kobayashi T, Kato-Itoh M, Nakauchi H, Hirabayashi M, Yagi T, Yoshimura Y.
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Journal Title
BMC Biol.
Volume: Dec 2;14(1)
Pages: 103-103
Related Report
Peer Reviewed / Open Access
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