Effects of overdose of fructose on dementia

• Project/Area Number: 26430055
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Kagawa University
• Principal Investigator: UENO Masaki 香川大学, 医学部, 教授 (30322267)
• Co-Investigator(Kenkyū-buntansha): 千葉 陽一 香川大学, 医学部, 講師 (30372113)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 果糖 / 脈絡叢 / グルコース輸送体 / 認知症 / GLUT / 活性酸素種 / 上衣細胞 / 培養細胞

Outline of Final Research Achievements

Immunoreactivity of GLUT5 and GLUT8 was located in epithelial cells of the choroid plexus and ependymal cells. Immunoreactivity of GLUT8 was located in the cytoplasm of astrocytes and microglia. It is likely that intravascular substance including sugars was transported to periventricular areas. These findings suggest that intravascular fructose migrates to periventricular areas. Culture experiments under high concentration of fructose for short and long periods were conducted by evaluating not only survival rates, production of reactive oxygen species (ROS), and effects on the outgrowth of cultured neurons, but also survival rates and ROS production of cultured microglia. Although the survival rate of cultured neurons for a long period had decreased slightly, other parameters were significantly changed.

Research Products

• [Journal Article] Glucose transporter 8 immunoreactivity in astrocytic and microglial cells in subependymal areas of human brains (2017)
  • Author(s): Mashima M, Chiba Y, Murakami R, Uemura N, Matsumoto K, Kawauchi M, Kanenishi K, Hata T, Ueno M
  • Journal Title: Neuroscience Letters Volume: 636 Pages: 90-94
  • DOI: 10.1016/j.neulet.2016.11.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Immunoreactivity of glucose transporter 8 is localized in the epithelial cells of the choroid plexus and in ependymal cells (2016)
  • Author(s): Murakami R, Chiba Y, Tsuboi K, Matsumoto K, Kawauchi M, Fujihara R, Mashima M, Kanenishi K, Yamamoto T, Ueno M
  • Journal Title: Histochemistry and Cell Biology Volume: 146 Issue: 2 Pages: 231-236
  • DOI: 10.1007/s00418-016-1444-5
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Albumin microvascular leakage in brains with diabetes mellitus (2016)
  • Author(s): Fujihara R, Chiba Y, Nakagawa T, Nishi N, Murakami R, Matsumoto K, Kawauchi M, Yamamoto T, Ueno M
  • Journal Title: Microscopy Research and Technique Volume: 79 Issue: 9 Pages: 833-837
  • DOI: 10.1002/jemt.22708
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Blood-brain barrier damage in vascular dementia (2016)
  • Author(s): Masaki Ueno, Yoichi Chiba, Koichi Matsumoto, Ryuta Murakami, Ryuji Fujihara, Machi Kawauchi, Hiroshi Miyanaka, Toshitaka Nakagawa
  • Journal Title: Neuropathology Volume: 36 Issue: 2 Pages: 115-124
  • DOI: 10.1111/neup.12262
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Blood-brain barrier and blood-cerebrospinal fluid barrier in normal and pathological conditions. (2016)
  • Author(s): Ueno M, Chiba Y, Murakami R, Matsumoto K, Kawauchi M, Fujihara R
  • Journal Title: Brain Tumor Pathol Volume: 33 Issue: 2 Pages: 89-96
  • DOI: 10.1007/s10014-016-0255-7
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of transporters related to clearance of amyloid-β peptides through blood-cerebrospinal fluid barrier in human brain. (2015)
  • Author(s): Matsumoto K, Chiba Y, Fujihara R, Kubo H, Sakamoto H, Ueno M
  • Journal Title: Histochem Cell Biol Volume: 144 Issue: 6 Pages: 597-611
  • DOI: 10.1007/s00418-015-1366-7
  • Peer Reviewed
• [Journal Article] 血液脳関門の構造的特徴と生理機能 (2015)
  • Author(s): 上野正樹
  • Journal Title: Dementia Japan Volume: 29 Pages: 36-43
• [Journal Article] Clearance of beta-amyloid in the brain (2014)
  • Author(s): Masaki Ueno, Yoichi Chiba, Koichi Matsumoto, Toshitaka Nakagawa, Hiroshi Miyanaka
  • Journal Title: Current Medicinal Chemistry Volume: 21 Issue: 35 Pages: 4085-4090
  • DOI: 10.2174/0929867321666141011194256
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 血液脳関門機能およびその障害の機序について (2015)
  • Author(s): 上野正樹
  • Organizer: 第33回日本脳腫瘍病理学会
  • Place of Presentation: JRホテルクレメント高松
  • Year and Date: 2015-05-29
  • Invited