| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Spikar is a transcriptional coactivator localized in dendritic spines and nucleus in neuron. We conducted real-time imaging of spikar in dendritic spines and analyses of spikar-KO mice. FRAP analysis showed that the dynamics of spikar in dendritic spine is regulated by actin-binding protein drebrin. In drebrin-knockdown neurons, the stable fraction of spikar was decreased, and the phenotype was rescued by drebrin-transfection. In the behavior analysis, we found abnormal maternal behaviors in spikar-KO mice. Spikar (-/-) females showed increased the time retrieving their pups and defect in grouping. Further, we analyzed transcriptome of spikar- KO mice brain by using microarray data. Gene ontology and pathway analyses revealed that the expression of numerous genes related with cytoskeleton and apoptosis signaling are regulated by spikar.
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