The elucidation of functions of CLAC-P/collagen XXV in neuromuscular development and synapse formation

• Project/Area Number: 26430064
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: The University of Tokyo
• Principal Investigator: Wakabayashi Tomoko 東京大学, 大学院医学系研究科(医学部), 助教 (20530330)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 運動ニューロン / 神経発生

Outline of Final Research Achievements

We previously identified that CLAC-P/collagen XXV is essential for intramuscular innervation of motor neurons during development. In this study, we generated tissue-specific Col25a1 knockout mice and revealed that collagen XXV expressed in developing skeletal muscle but not in motor neuron is indispensable for neuromuscular development. mRNA expression of collagen XXV in skeletal muscle is downregulated by muscle electrical activity arising from motor innervation. We also identified type IIa RPTPs, which are known to be involved in synapse formation in the CNS and motor axon elongation/regeneration, as possible interactors.

Research Products

• [Presentation] Identification of the interactor of CLAC-P/Collagen type XXV in motor nerve innervation of skeletal muscles (2016)
  • Author(s): Haruka Munezane, Hiroaki Oizumi, Tomoyuki Yoshida, Tomoko Wakabayashi, Takeshi Iwastubo
  • Organizer: Neuroscience 2016
  • Place of Presentation: San Diego, CA (U.S.A.)
  • Year and Date: 2016-11-12
  • Int'l Joint Research
• [Presentation] The elucidation of the physiological function of CLAC-P/ collagen XXV in neuromuscular development (2016)
  • Author(s): Haruka Munezane, Hiroaki Oizumi, Tomoyuki Yoshida, Tomoko Wakabayashi, Takeshi Iwastubo
  • Organizer: The 47th NIPS International Symposium
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
  • Year and Date: 2016-10-27
  • Int'l Joint Research
• [Presentation] The elucidation of molecular mechanism of CLAC-P/Collagen type XXV in motor innervation of skeletal muscle (2015)
  • Author(s): Haruka Munezane, Hiroaki Oizumi, Tomoyuki Yoshida, Tomoko Wakabayashi, Takeshi Iwastubo
  • Organizer: BMB2015
  • Place of Presentation: 神戸ポートアイランド（兵庫県神戸市）
  • Year and Date: 2015-12-01
• [Presentation] Collagen XXV/CLAC-Pの骨格筋における発現制御に関する研究 (2015)
  • Author(s): 西尾 周、大泉 寛明、宗實 悠佳、若林 朋子、岩坪 威
  • Organizer: 第34回 日本認知症学会学術集会
  • Place of Presentation: リンクステーションホール青森（青森県青森市）
  • Year and Date: 2015-10-02
• [Presentation] Roles in Neuromuscular Development of CLAC-P/Collagen Type XXV: A Molecule Originally Identified in Alzheimer Brains (2015)
  • Author(s): Tomoko Wakabayashi
  • Organizer: Gordon Research Conference: Collagen
  • Place of Presentation: New London, NH ( U.S.A.)
  • Year and Date: 2015-07-12
  • Int'l Joint Research / Invited
• [Presentation] アルツハイマー病脳老人斑構成成分 CLAC-P/CollagenXXV の 中枢神経系における生理機能の解明 (2014)
  • Author(s): 宗実悠佳、吉田知之、若林朋子、岩坪威
  • Organizer: 第33回日本認知症学会学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2014-11-29 – 2014-12-01
• [Presentation] CLAC-P/collagen type XXV in muscle, but not in motor neurons, is required for intramuscular development of motor axons (2014)
  • Author(s): Oizumi H, Tanaka T, Nishio S, Munezane H, Hashimoto T, Harada A, Wakabayashi T, Iwatsubo T
  • Organizer: Society for Neuroscience 2014
  • Place of Presentation: Washington DC, U.S.A.
  • Year and Date: 2014-11-15 – 2014-11-19