Molecular basis of lifespan and aging by exposing LED light in C. elegans

• Project/Area Number: 26430068
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Kanazawa University
• Principal Investigator: Shin-ichi Harada 金沢大学, 医学系, 助教 (90272955)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: C. elegans / LED light / LED / 寿命

Outline of Final Research Achievements

To invesigate the biological disadvantage of blue LED light, we used the free-living nematode Caenorhabditis elegans, as a model. By exposing blue LED light at 465nm, the lifespan of C. elegans was decreased, and these reactive oxygen species (ROS) activity as a marker of stress response were slightly increased. Using Real-Time RT-PCR analysis to measure gene expression levels by exposure of LED light, we found that mec-5 gene which express in sensory neuron was highly expressed and neural calcium sensor (ncs-1) gene was significantly decreased in worms with blue LED light as compared to dark exposure as a control. Furthermore, we found to cause a marked deterioration in the worm's behavior with blue LED light. It is concluded that exposure of blue LED light may cause oxidative stress, and result in abnormal locomotion and survival in the nematode C. elegans. However, further investigation is required to clarify the toxicological effect of the LEDs light.

Research Products

• [Journal Article] Low-dose paclitaxel suppresses the induction of M2 macrophages in gastric cancer (2017)
  • Author(s): Yamaguchi T, Fushida S, Yamamoto Y, Tsukada T, Kinoshita J, Oyama K, Miyashita T, Tajima H, Ninomiya I, Munesue S, Harashima A, Harada S, Yamamoto H, Ohta T
  • Journal Title: Oncology Reports Volume: - Issue: 6 Pages: 3341-3350
  • DOI: 10.3892/or.2017.5586
  • Peer Reviewed
• [Journal Article] Extravasated platelet aggregation in the livers of rats with drug-induced hepatic sinusoidal obstruction syndrome (2017)
  • Author(s): Hirata M, Tajima H, Miyashita T, Miyata T, Nakanuma S, Makino I, Hayashi H, Oyama K, Takamura H, Ninomiya I, Fushida S, Nakata H, Iseki S, Harada S, Wakayama T, Ohta T
  • Journal Title: Molecular Medicine Reports Volume: 15(5) Issue: 5 Pages: 3147-3152
  • DOI: 10.3892/mmr.2017.6407
  • NAID: 120006306225
  • Peer Reviewed
• [Journal Article] Establishing a xenograft mouse model of peritoneal dissemination of gastric cancer with organ invasion and fibrosis (2017)
  • Author(s): Okazaki M, Fushida S, Harada S, Tsukada T, Kinoshita J, Oyama K, Miyashita T, Ninomiya I, Ohta T
  • Journal Title: BMC Cancer Volume: 17(1) Issue: 1 Pages: 23-23
  • DOI: 10.1186/s12885-016-2991-9
  • Peer Reviewed / Open Access
• [Journal Article] Valproic acid inhibits irradiation-induced epithelial-mesenchymal transition and stem cell-like characteristics in esophageal squamous cell carcinoma (2016)
  • Author(s): Kanamoto A, Ninomiya I, Harada S, Tsukada T, Okamoto K, Nakanuma S, Sakai S, Makino I, Kinoshita J, Hayashi H, Oyama K, Miyashita T, Tajima H, Takamura H, Fushida S, Ohta T
  • Journal Title: Int J Oncol. Volume: 49(5) Issue: 5 Pages: 1859-1869
  • DOI: 10.3892/ijo.2016.3712
  • Peer Reviewed / Open Access
• [Journal Article] Metformin inhibits the radiation-induced invasive phenotype of esophageal squamous cell carcinoma (2016)
  • Author(s): Nakayama A, Ninomiya I, Harada S, Tsukada T, Okamoto K, Nakanuma S, Sakai S, Makino I, Kinoshita J, Hayashi H, Oyama K, Miyashita T, Tajima H, Takamura H, Fushida S, Ohta T
  • Journal Title: Int J Oncol. Volume: 49(5) Issue: 5 Pages: 1890-1898
  • DOI: 10.3892/ijo.2016.3676
  • Peer Reviewed / Open Access
• [Journal Article] Concentration-dependent radiosensitizing effect of docetaxel in esophageal squamous cell carcinoma cells. (2015)
  • Author(s): Miyanaga S, Ninomiya I, Tsukada T, Okamoto K, Harada S, Nakanuma S, Sakai S, Makino I, Kinoshita J, Hayashi H, Nakamura K, Oyama K, Nakagawara H, Miyashita T, Tajima H, Takamura H, Fushida S, Ohta T
  • Journal Title: Int J Oncol. Volume: 48(2) Issue: 2 Pages: 517-524
  • DOI: 10.3892/ijo.2015.3291
  • Peer Reviewed / Open Access
• [Journal Article] Valproic acid inhibits proliferation of HER2-expressing breast cancer cells by inducing cell cycle arrest and apoptosis through Hsp70 acetylation. (2015)
  • Author(s): Mawatari T, Ninomiya I, Inokuchi M, Harada S, Hayashi H, Oyama K, Makino I, Nakagawara H, Miyashita T, Tajima H, Takamura H, Fushida S, Ohta T
  • Journal Title: Int J Oncol. Volume: 47(6) Issue: 6 Pages: 2073-2081
  • DOI: 10.3892/ijo.2015.3213
  • Peer Reviewed / Open Access
• [Journal Article] Inhibitory effects of valproic acid in DNA double-strand break repair after irradiation in esophageal squamous carcinoma cells. (2015)
  • Author(s): Makita N, Ninomiya I, Tsukada T, Okamoto K, Harada S, Nakanuma S, Sakai S, Makino I, Kinoshita J, Hayashi H, Oyama K, Nakagawara H, Miyashita T, Tajima H, Takamura H, Fushida S, Ohta T
  • Journal Title: Oncol Rep. Volume: 34(3) Issue: 3 Pages: 1185-1192
  • DOI: 10.3892/or.2015.4089
  • Peer Reviewed / Open Access
• [Journal Article] Chemoprevention of esophageal adenocarcinoma in a rat model by ursodeoxycholic acid. (2015)
  • Author(s): Ojima E, Fujimura T, Oyama K, Tsukada T, Kinoshita J, Miyashita T, Tajima H, Fushida S, Harada S, Mukaisho K, Hattori T, Ohta T
  • Journal Title: Clin Exp Med. Volume: 15(3) Issue: 3 Pages: 343-350
  • DOI: 10.1007/s10238-014-0301-x
  • Peer Reviewed / Open Access
• [Journal Article] Bone marrow derived "fibrocytes" contribute to tumor proliferation and fibrosis in gastric cancer. (2015)
  • Author(s): erai S, Fushida S, Tsukada T, Kinoshita J, Oyama K, Okamoto K, Makino I, Tajima H, Ninomiya I, Fujimura T, Harada S, Ohta T
  • Journal Title: Gastric Cancer Volume: 18(2) Issue: 2 Pages: 306-313
  • DOI: 10.1007/s10120-014-0380-0
  • Peer Reviewed / Open Access
• [Journal Article] Protein-bound polysaccharide K suppresses tumor fibrosis in gastric cancer by inhibiting the TGF-β signaling pathway. (2015)
  • Author(s): Shinbo T, Fushida S, Tsukada T, Harada S, Kinoshita J, Oyama K, Okamoto K, Ninomiya I, Takamura H, Kitagawa H, Fujimura T, Yashiro M, Hirakawa K, Ohta T.
  • Journal Title: Oncol Rep. Volume: 33(2) Issue: 2 Pages: 553-558
  • DOI: 10.3892/or.2014.3636
  • Peer Reviewed
• [Journal Article] The angiotensin II type 1 receptor blocker candesartan suppresses proliferation and fibrosis in gastric cancer. (2014)
  • Author(s): Okazaki M, Fushida S, Harada S, Tsukada T, Kinoshita J, Oyama K, Tajima H, Ninomiya I, Fujimura T, Ohta T.
  • Journal Title: Cancer Lett. Volume: 355(1) Issue: 1 Pages: 46-53
  • DOI: 10.1016/j.canlet.2014.09.019
  • Peer Reviewed