Systematic genetic analysis for urethane-induced lung cancer susceptibility loci in mice
| Project/Area Number |
26430088
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory animal science
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
Ohno Tamio 名古屋大学, 医学系研究科, 准教授 (90293620)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
TOYOKUNI Shinya 名古屋大学, 大学院医学系研究科, 教授 (90252460)
UNNO Akihiro 名古屋大学, 大学院医学系研究科, 助教 (50628592)
|
|---|
| Research Collaborator |
MIYASAKA Yuki 名古屋大学, 大学院医学系研究科, 助教 (30778098)
MAEGAWA Tomoki
|
|---|
| Project Period (FY) |
2014-04-01 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | マウス / 体系的遺伝解析系 / コンソミック系統 / コンジェニック系統 / ウレタン誘発肺腫瘍 / 肺腫瘍感受性遺伝子 |
|---|
| Outline of Final Research Achievements |
QTL analysis using SMXA・RI strains revealed plumonary adenoma resistance locus 1 (Par1) on Chr.11 and Par3 on Chr.12. However, it could not be confirmed the existence and effects of these loci by using congenic strains developed in this study. We conducted re-mapping by the Chr. 11 congenic strains, and indicated that the other Par locus was located between 27.72Mb and 36.39Mb. The Mpg gene, which encodes a ubiquitous DNA repair enzyme associated with lung cancer, is located within this region. A Sanger sequence analysis of the Mpg gene revealed five polymorphic sites in the A/J genome. One variant, p.Ala132Ser, was suggested the strong candidate mutation with regard to susceptibility to urethan-induced lung cancer.
|
Report
(5 results)
Research Products
(2 results)
