Mechanism of diabetes development due to dysfunction of unfolded protein response
| Project/Area Number |
26430090
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
Saito Michiko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 客員准教授 (40379558)
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| Research Collaborator |
Tsuchiya Yuichi
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 小胞体ストレス / 糖尿病 / インスリン / 小胞体ストレス応答 |
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| Outline of Final Research Achievements |
The endoplasmic reticulum (ER) is an organelle where newly synthesized secretory and membrane proteins are folded and assembled. Various stresses cause the accumulation of unfolded proteins in the ER, resulting in dysfunction of the ER. This condition is called ER stress. ER stress sensors are activated in pancreatic β cells, and loss of these sensors causes diabetes. Although the function of each sensor has been well studied, their precise roles in animal tissues are largely unknown.
To examine this physiological role of the IRE1α (one of the ER stress sensors) in pancreatic β cells, we generated pancreatic-β-cell-specific IRE1α conditional KO (CKO) mice and IRE1α-CKO insulinoma cell lines. IRE1α CKO mice developed insulin-dependent diabetes. We found that expression of some genes encoding folding enzymes was decreased in the insulinoma cells. From these observations, we concluded that IRE1α was found to be necessary for the folding process of insulin.
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] 4μ8C Inhibits Insulin Secretion Independent of IRE1α RNase Activity2017
Author(s)
Sato, H., Shiba, Y., Tsuchiya, Y., Saito, M., Kohno, K.
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Journal Title
Cell Structure and Function
Volume: 42
Issue: 1
Pages: 61-70
DOI
NAID
ISSN
0386-7196, 1347-3700
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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