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Development of antibody-inducing vaccine models by analysis of B404 which neutralizes various SIV strains

Research Project

Project/Area Number 26430091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Laboratory animal science
Research InstitutionKumamoto University

Principal Investigator

Kuwata Takeo  熊本大学, エイズ学研究センター, 助教 (70346063)

Co-Investigator(Renkei-kenkyūsha) MATSUSHITA Shuzo  熊本大学, エイズ学研究センター, 教授 (00199788)
IGARASHI Tatsuhiko  京都大学, ウイルス研究所, 教授 (90467431)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsantibody / SIV / vaccine / HIV / 中和抗体 / アカゲザル / ワクチン
Outline of Final Research Achievements

SIV-infected macaques, which is an animal model of HIV infection, was used for analysis to develop antibody-inducing vaccines. Binding of potent anti-SIV neutralizing antibody B404 was not affected by point mutations on Env, and was suggested to require the structure of antigen-binding domain with negative charges. The biased induction of B404-class antibodies in macaques suggested the effect of host factors in antibody induction. Moreover, maturation of antibodies resulted in increase of neutralization activity against homologous virus, but did not associated with cross-neutralization activity against heterologous viruses

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (22 results)

All 2016 2015 2014 Other

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (15 results) (of which Int'l Joint Research: 3 results) Remarks (2 results)

  • [Journal Article] Enhanced antibody-mediated neutralization of HIV-1 variants that are resistant to fusion inhibitors2016

    • Author(s)
      Alam, M., Kuwata, T., Shimura, K., Yokoyama, M., Ramirez Valdez, K. P., Tanaka, K., Maruta, Y., Oishi, S., Fujii, N., Sato, H., Matsuoka M. and Matsushita S.
    • Journal Title

      Retrovirology

      Volume: 13 Issue: 1 Pages: 70-70

    • DOI

      10.1186/s12977-016-0304-7

    • NAID

      120005973385

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Cross-Neutralization Activity of Single-Chain Variable Fragment (scFv) Derived from Anti-V3 Monoclonal Antibodies Mediated by Post-Attachment Binding2016

    • Author(s)
      Maruta, Y., Kuwata, T., Tanaka, K., Alam, M., Valdez, K. P., Egami, Y., Yoshiaki Suwa, Y., Morioka, H. and Matsushita, S.
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 69 Issue: 5 Pages: 395-404

    • DOI

      10.7883/yoken.JJID.2015.667

    • NAID

      130005416280

    • ISSN
      1344-6304, 1884-2836
    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Incompatible Natures of the HIV-1 Envelope in Resistance to the CCR5 Antagonist Cenicriviroc and to Neutralizing Antibodies2016

    • Author(s)
      Kuwata T, Enomoto I, Baba M, Matsushita S
    • Journal Title

      Antimicrob Agents Chemother

      Volume: 60 Issue: 1 Pages: 437-450

    • DOI

      10.1128/aac.02285-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Immunoglobulin VH gene diversity and somatic hypermutation during SIV infection of rhesus macaques2015

    • Author(s)
      Guo K, Halemano K, Schmitt K, Katuwal M, Wang Y, Harper MS, Heilman KJ, Kuwata T, Stephens EB, Santiago ML
    • Journal Title

      Immunogenetics

      Volume: 67 Issue: 7 Pages: 355-370

    • DOI

      10.1007/s00251-015-0844-3

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Complementary and synergistic activities of anti-V3, CD4bs and CD4i antibodies derived from a single individual can cover a wide range of HIV-1 strains.2015

    • Author(s)
      Ramirez, K.P., Kuwata, T., Maruta, Y, Tanaka K., Muntasir, A., Yoshimura, K., Matsushita, S.
    • Journal Title

      Virology

      Volume: 475 Pages: 187-203

    • DOI

      10.1016/j.virol.2014.11.011

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] CD4+T細胞の分化に対するHIV感染とmiR125bの影響の検討2016

    • Author(s)
      郭悠、南留美、小松真梨子、高濱宗一郎、高濱正吉、桑田岳夫、山本政弘、松下修三
    • Organizer
      第30回日本エイズ学会学術集会・総会
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Dynamics of HIV-1 DNA in long-term treated patients with hematological co-morbidities2016

    • Author(s)
      Stanoeva K, Konig A, Fukuda A, Kawanami Y, Kuwata T, Satou Y, Matsushita S
    • Organizer
      第30回日本エイズ学会学術集会・総会
    • Place of Presentation
      鹿児島
    • Year and Date
      2016-11-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] Analysis and characterization of HIV-1 envelope glycoprotein in Japanese patients with recent diagnosis2016

    • Author(s)
      Thida W, Kuwata T, Alam M, Tanaka K, Maun M, Shimizu M, Kawanami Y and Matsushita S
    • Organizer
      17th Kumamoto AIDS Seminar
    • Place of Presentation
      熊本
    • Year and Date
      2016-10-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] Construction, purification and analysis of neutralization potency of anti-V3 scFv’s against HIV-1 strains in vitro2016

    • Author(s)
      Mamun M, Maruta Y, Tanaka K, Alam M, Thida W, Kuwata T and Matsushita S
    • Organizer
      17th Kumamoto AIDS Seminar
    • Place of Presentation
      熊本
    • Year and Date
      2016-10-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] Induction of antibodies, which belonged to the same class as broadly neutralizing antibody B404, in macaques infected with SIVsmH635FC2016

    • Author(s)
      Kuwata T, Sano M, Matsuoka S, Shimizu M, Miura T, Seki Y, Akari H, Matano T and Matsushita S
    • Organizer
      17th Kumamoto AIDS Seminar
    • Place of Presentation
      熊本
    • Year and Date
      2016-10-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] PM1/CCR5 cells transduced with T312 Intrabody have survival advantage, if challenged with SF162 HIV-1 strain2016

    • Author(s)
      Alves G, Kuwata T, Tanaka Y, Matsushita S
    • Organizer
      17th Kumamoto AIDS Seminar
    • Place of Presentation
      熊本
    • Year and Date
      2016-10-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] Post-attachment neutralization of a broad range of HIV-1 strains by the small antibody fragments targeting CD4-induced (CD4i) epitope2016

    • Author(s)
      Tanaka K, Kuwata T, Takahama S, Alam M, Maruta Y, Ramirez Valdez K P, Shimizu M, Kawanami Y and Matsushita S
    • Organizer
      17th Kumamoto AIDS Seminar
    • Place of Presentation
      熊本
    • Year and Date
      2016-10-31
    • Related Report
      2016 Annual Research Report
  • [Presentation] Post-attachment Neutralization of a Wide Range of HIV-1 Strains by Anti-CD4i Single Chain Variable Fragment (scFv)2016

    • Author(s)
      Tanaka K, Kuwata T, Maruta Y, Ramirez Valdez K P, Alam M, Kawanami Y, Matsushita S
    • Organizer
      HIVR4P 2016
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2016-10-17
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] HIV proviral DNA quantification in a cohort of Japanese patients on long-term ART2016

    • Author(s)
      Stanoeva K, Konig A, Fukuda A, Kawanami Y, Kuwata T, Satou Y, Matsushita S
    • Organizer
      2016 Towards an HIV Cure Symposium
    • Place of Presentation
      Durban, South Africa
    • Year and Date
      2016-07-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Incompatibility of HIV-1 Resistance to Both Cenicriviroc and Neutralizing Antibodies2016

    • Author(s)
      Kuwata T, Enomoto I, Baba M and Matsushita S
    • Organizer
      The annual Conference on Retroviruses and Opportunistic Infections
    • Place of Presentation
      Boston, USA
    • Year and Date
      2016-02-22
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Impact of gp41 mutations on resistance to B404, a broad neutralizing antibody reactive against a conformational epitope of SIV gp1202015

    • Author(s)
      Kuwata T, Shimizu M, Matsushita S
    • Organizer
      第63回日本ウイルス学会学術集会
    • Place of Presentation
      福岡
    • Year and Date
      2015-11-22
    • Related Report
      2015 Research-status Report
  • [Presentation] Impact of gp41 mutations for escape from a broad neutralizing antibody against SIV2015

    • Author(s)
      Kuwata T, Shimizu M, Matsushita S
    • Organizer
      16th Kumamoto AIDS Seminar
    • Place of Presentation
      熊本
    • Year and Date
      2015-10-07
    • Related Report
      2015 Research-status Report
  • [Presentation] 強力な抗SIV中和抗体B404からの逃避メカニズムの解析2014

    • Author(s)
      桑田岳夫、松下修三
    • Organizer
      第28回日本エイズ学会学術集会・総会
    • Place of Presentation
      大阪国際会議場(大阪府北区)
    • Year and Date
      2014-12-03 – 2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] Mutations in gp41 is Critical to Escape from B404, a Broad Neutralizing Antibody Against SIV, Which Recognizes a V3/V4 Conformational Epitope2014

    • Author(s)
      Takeo Kuwata, Kazuhisa Yoshimura, Fan Wu, Ilnour Ourmanov, Vanessa M. Hirsch, Masaru Yokoyama, Hironori Sato and Shuzo Matsushita
    • Organizer
      32nd Annual Symposium on Nonhuman Primate Models for AIDS
    • Place of Presentation
      The Nines Hotel (Portland, Oregon, USA)
    • Year and Date
      2014-11-11 – 2014-11-14
    • Related Report
      2014 Research-status Report
  • [Presentation] SIVサル・モデルを用いた中和抗体の解析2014

    • Author(s)
      桑田岳夫
    • Organizer
      第17回日本レトロウイルス研究会夏期セミナー
    • Place of Presentation
      伊豆山研修センター(静岡県熱海市)
    • Year and Date
      2014-07-03 – 2014-07-05
    • Related Report
      2014 Research-status Report
  • [Remarks] 松下プロジェクト研究室 - 熊本大学エイズ学研究センター

    • URL

      http://matsushita-lab.jp

    • Related Report
      2015 Research-status Report
  • [Remarks] 熊本大学 エイズ学研究センター 松下プロジェクト研究室

    • URL

      http://matsushita-lab.kumamoto-univ.jp

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2018-03-22  

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