Search for miRNAs that regulate stemness of breast cancer stem cell to find therapeutic targets of triple-negative breast cancer.
| Project/Area Number |
26430109
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Tumor biology
|
|---|
| Research Institution | Chiba University (2016)
The University of Tokyo (2014-2015) |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
伊庭 英夫 千葉大学, 真菌医学研究センター, 特任教授 (60111449)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
|
|---|
| Keywords | microRNA / microRNA inhibitor / 癌幹細胞 / EMT / 乳癌 / トリプルネガティブ |
|---|
| Outline of Final Research Achievements |
Triple negative breast cancer cell line SUM149PT is composed of 2 subpopulations; epithelial-like EpCAM positive (EpCAM+) cells and mesenchymal-like EpCAM negative (EpCAM-) cells. In this study, we found that EpCAM+ cells had strong tumorigenicity and the expression levels of miR-200 family were high in only EpCAM+ cells.
When we suppressed the miR-200 family in EpCAM+ cells, conversion of EpCAM+ cells to EpCAM- cells occurred and the tumorigenicity of the EpCAM+ cells was reduced. Furthermore, we developed and used doxycycline-inducible miRNA inhibitory vector to construct EpCAM+ cell in which the activities of miR-200 family can be regulated by doxycycline. After tumor formation in xenograft mice transplanted with these cells, we inhibited miR-200 family and observed tumor regression. These results showed that miR-200 family was promising therapeutic targets of triple negative breast cancer.
|
Report
(4 results)
Research Products
(12 results)
-
-
[Journal Article] Dynamics and plasticity of the epithelial to mesenchymal transition induced by miR-200 family inhibition2016
Author(s)
Haraguchi, T, Kondo, M, Uchikawa, R, Kobayashi, K, Hiramatsu, H, Kobayashi, K , Chit, UW, Shimizu, T, Iba, H.
-
Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 1-12
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
-
[Journal Article] The miR-199a/Brm/EGR1 axis is a determinant of anchorage-independent growth in epithelial tumor cell lines.2015
Author(s)
Kobayashi, K., Sakurai, K., Hiramatsu, H., Inada, K., Shiogama, K., Nakamura, S., Suemasa, F., Kobayashi, K., Imoto, S., Haraguchi, T., Ito, H., Ishizaka, A., Tsutsumi, Y., & Iba H.
-
Journal Title
Scientific Reports,
Volume: 5:8428
Issue: 1
Pages: 8428-8428
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
