Research Project
Grant-in-Aid for Scientific Research (C)
Triple negative breast cancer cell line SUM149PT is composed of 2 subpopulations; epithelial-like EpCAM positive (EpCAM+) cells and mesenchymal-like EpCAM negative (EpCAM-) cells. In this study, we found that EpCAM+ cells had strong tumorigenicity and the expression levels of miR-200 family were high in only EpCAM+ cells.When we suppressed the miR-200 family in EpCAM+ cells, conversion of EpCAM+ cells to EpCAM- cells occurred and the tumorigenicity of the EpCAM+ cells was reduced. Furthermore, we developed and used doxycycline-inducible miRNA inhibitory vector to construct EpCAM+ cell in which the activities of miR-200 family can be regulated by doxycycline. After tumor formation in xenograft mice transplanted with these cells, we inhibited miR-200 family and observed tumor regression. These results showed that miR-200 family was promising therapeutic targets of triple negative breast cancer.
All 2017 2016 2015 2014 Other
All Journal Article (3 results) (of which Peer Reviewed: 3 results, Open Access: 3 results, Acknowledgement Compliant: 1 results) Presentation (7 results) Remarks (2 results)
Sienctific Reports
Volume: 7(1) Issue: 1 Pages: 889-889
10.1038/s41598-017-00982-3
Scientific Reports
Volume: 6 Issue: 1 Pages: 1-12
10.1038/srep21117
Scientific Reports,
Volume: 5:8428 Issue: 1 Pages: 8428-8428
10.1038/srep08428
http://www.pf.chiba-u.ac.jp/research/project/iba.html
http://www.ims.u-tokyo.ac.jp/div-host-parasite/version1.html
