| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
Dysregulation of the centrosome cycle promotes the chromosomal instability (CIN), one of the hallmarks of tumor malignancy, thereby inducing centrosome overduplication, multipolar spindle formation, and chromosome missegregation. This suggests that certain centrosomal kinases stringently regulate the mitotic checkpoint. In this study, we found that the centrosomal kinases, Lats1 and Lats2, regulate centrosome duplication, cytokinesis, accurate chromosome segregation, and a nuclear function after DNA damage by phosphorylating various target proteins, such as Cdc25B, CHO1/MKLP1, and INCENP, thereby avoiding the induction of CIN. Therefore, we proposed a model in which the centrosomes function not only as a mitotic spindle pole for microtubule nucleation but also as an important site for crosstalk of signaling pathways in the mitotic checkpoint.
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