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Aerotaxis in cancer cells

Research Project

Project/Area Number 26430117
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor biology
Research InstitutionSaga University

Principal Investigator

Takahashi Eiji  佐賀大学, 工学(系)研究科(研究院), 教授 (30206792)

Research Collaborator YAHARA Disuke  佐賀大学, 大学院工学系研究科, 大学院生
YOSHIDA Takuma  佐賀大学, 大学院工学系研究科, 大学院生
ENOKIDA Yuya  佐賀大学, 大学院工学系研究科, 大学院生
OKUBO Kaima  佐賀大学, 大学院工学系研究科, 大学院生
TSURUNO Yusuke  佐賀大学, 大学院工学系研究科, 大学院生
FUJIWARA Takahiro  佐賀大学, 大学院工学系研究科, 大学院生
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsがん細胞 / 血行性遠隔転移 / 酸素 / pH / 細胞遊走 / 低酸素微小環境 / がん転移 / 酸素濃度勾配 / pH勾配 / イメージング / MDA-MB-231 / 腫瘍細胞遊走 / 転移 / 酸素測定
Outline of Final Research Achievements

To elucidate the initial step of hematogenous metastasis of tumors, we hypothesized that migration of tumor cells might be directed by extracellular gradients of metabolic substrates/metabolites particularly by oxygen and/or H+. We first developed a novel technique to produce metabolic gradients in monolayers of cultured cell (MDA-MB-231 cells) in which cell migrations can be tracked for up to 24 hrs. We found significant directional migrations of the cell toward higher oxygen concentration or lower H+ concentration. These directional migrations were demonstrated in unphysiologically high oxygen concentrations (eg. 21%) while changes in extracellular H+ gradients in physiological ranges appeared to affect the directionality. From these results, we conclude that migrations of MDA-MB-231 cells in vitro seem to be directed by extracellular pH gradients.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (36 results)

All 2017 2016 2015 2014 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results,  Acknowledgement Compliant: 3 results) Presentation (26 results) (of which Int'l Joint Research: 6 results,  Invited: 8 results) Book (1 results) Remarks (6 results)

  • [Journal Article] Directional migration of MDA-MB-231 cells under O2/pH gradients.2017

    • Author(s)
      Enokida Y, Tsuruno Y, Okubo K, Yamaoka Y, Takahashi E
    • Journal Title

      Adv Exp Med Biol

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Directional migration of MDA-MB-231 cells under oxygen concentration gradients.2016

    • Author(s)
      Yahara D, Yoshida T, Enokida Y, Takahashi E
    • Journal Title

      Adv Exp Med Biol

      Volume: 923 Pages: 129-134

    • DOI

      10.1007/978-3-319-38810-6_17

    • ISBN
      9783319388083, 9783319388106
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Directional migration of MDA-MB-231 cell under oxygen concentration gradients.2016

    • Author(s)
      Yahara D, Yoshida T, Enokida Y, Takahashi E
    • Journal Title

      Advances in Experimental Medicine and Biology

      Volume: XXX

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 培養細胞培地に形成されるO2勾配のイメージング2017

    • Author(s)
      大久保魁馬、靏野雄介、藤原隆祐、榎田祐也、山岡禎久、高橋英嗣
    • Organizer
      日本生体医工学会九州支部学術講演会
    • Place of Presentation
      福岡市
    • Year and Date
      2017-03-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] Metabolic gradients下の細胞遊走2016

    • Author(s)
      靏野雄介, 大久保魁馬, 藤原隆祐, 榎田祐也, 山岡禎久,高橋英嗣
    • Organizer
      第20回酸素ダイナミクス研究会
    • Place of Presentation
      東京都
    • Year and Date
      2016-11-19
    • Related Report
      2016 Annual Research Report
  • [Presentation] MDA-MB-231細胞はmetabolic gradient下で方向性に遊走する2016

    • Author(s)
      靏野雄介, 大久保魁馬, 藤原隆祐, 榎田祐也, 高橋英嗣
    • Organizer
      第14回がんとハイポキシア研究会
    • Place of Presentation
      岐阜市
    • Year and Date
      2016-11-04
    • Related Report
      2016 Annual Research Report
  • [Presentation] がんの遠隔転移:がん細胞は酸素をもとめて遊走するか?2016

    • Author(s)
      高橋英嗣
    • Organizer
      酸素ダイナミックスに関するセミナー
    • Place of Presentation
      郡山
    • Year and Date
      2016-10-14
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Imaging of oxygen in single cardiac myocyte reveals intracellular trafficking of energy2016

    • Author(s)
      Takahashi E
    • Organizer
      Ireland-Japan Joint Seminar: Membrane trafficking, cilia and oxygen sensing
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2016-10-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Mitochondrial membrane potential in DMOG model of metabolic reprogramming.2016

    • Author(s)
      Takahashi E
    • Organizer
      ISOTT2016
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2016-07-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Directional migration of MDA-MB-231 cells under O2/pH gradients.2016

    • Author(s)
      Enokida Y, Tsuruno Y, Okubo K, Yamaoka Y, Takahashi E
    • Organizer
      ISOTT2016
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2016-07-11
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Directional migration of MDA-MB-231 cells under O2/pH gradients2016

    • Author(s)
      Y.Enokida, Y. Tsuruno, K. Okubo, Y. Yamaoka and E. Takahashi
    • Organizer
      ISOTT2016
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2016-07-10
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] MDA-MB-231細胞遊走のpH勾配依存性2016

    • Author(s)
      榎田 祐也, 矢原 大裕, 吉田 拓磨, 靏野 雄介, 大久保 魁馬, 馬場 嵐,山岡 禎久,高橋 英嗣
    • Organizer
      第55回日本生体医工学会大会
    • Place of Presentation
      富山市
    • Year and Date
      2016-04-28
    • Related Report
      2015 Research-status Report
  • [Presentation] MDA-MB-231細胞遊走のpH依存性2016

    • Author(s)
      榎田 祐也, 矢原 大裕, 吉田 拓磨, 靏野 雄介, 大久保 魁馬, 馬場 嵐,山岡 禎久, 高橋 英嗣
    • Organizer
      第55回日本生体医工学会大会
    • Place of Presentation
      富山市
    • Year and Date
      2016-04-27
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酸素の生理学と臓器再生工学への応用2016

    • Author(s)
      高橋英嗣
    • Organizer
      2016年日本生体医工学会九州支部学術講演会
    • Place of Presentation
      佐賀市
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] メタボリックリプログラミングによる低酸素細胞のサバイバル2015

    • Author(s)
      高橋英嗣
    • Organizer
      第49回日本生体医工学会東北支部大会
    • Place of Presentation
      郡山市
    • Year and Date
      2015-11-21
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 腫瘍細胞遊走の酸素濃度勾配依存性2015

    • Author(s)
      矢原 大裕, 吉田 拓磨, 榎田 祐也, 大久保 魁馬, 靏野 雄介, 馬場 嵐, 高橋 英嗣
    • Organizer
      第66回西日本生理学会
    • Place of Presentation
      久留米市
    • Year and Date
      2015-10-09
    • Related Report
      2015 Research-status Report
  • [Presentation] Gap cover glass (GCG)を用いて形成した低酸素微小環境中のpHイメージング2015

    • Author(s)
      榎田 祐也, 吉田 拓磨, 矢原 大裕, 高橋 英嗣
    • Organizer
      生体医工学シンポジウム2015
    • Place of Presentation
      岡山市
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 酸素濃度勾配に沿ったMDA-MB-231細胞の遊走2015

    • Author(s)
      矢原 大裕, 吉田 拓磨, 榎田 祐也, 大久保 魁馬, 靏野 雄介, 馬場 嵐, 高橋 英嗣
    • Organizer
      第19回酸素ダイナミクス研究会
    • Place of Presentation
      東京都
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] Directional migration of MDA-MB-231 cell under oxygen concentration gradients2015

    • Author(s)
      Yahara D, Yoshida T, Enokida Y, Takahashi E
    • Organizer
      ISOTT2015
    • Place of Presentation
      Wuhan, China
    • Year and Date
      2015-07-13
    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Int'l Joint Research
  • [Presentation] Life with less oxygen: role of prolyl hydroxylase pathway activation in pHi stabilization in a cancer cell line2015

    • Author(s)
      Takahashi E, Enokida Y.
    • Organizer
      ISOTT2015
    • Place of Presentation
      Wuhan, China
    • Year and Date
      2015-07-13
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] 酸素濃度勾配下での腫瘍細胞の方向性遊走2015

    • Author(s)
      矢原大裕、吉田拓磨、榎田祐也、高橋英嗣
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-05-08
    • Related Report
      2015 Research-status Report
  • [Presentation] Gap cover glass (GCG)を用いた2次元低酸素微小環境モデル2015

    • Author(s)
      吉田拓磨、矢原大裕、榎田祐也、高橋英嗣
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-05-08
    • Related Report
      2015 Research-status Report
  • [Presentation] 酸素濃度勾配下での腫瘍細胞の方向性遊走2015

    • Author(s)
      矢原大裕,吉田拓磨,榎田祐也,高橋英嗣
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-05-08
    • Related Report
      2014 Research-status Report
  • [Presentation] Gap Cover Glass(GCG)を用いた培養細胞組織内酸素濃度勾配形成2015

    • Author(s)
      吉田拓磨,矢原大裕,榎田祐也,高橋英嗣
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-05-08
    • Related Report
      2014 Research-status Report
  • [Presentation] チュートリアル:生体、組織、細胞の酸素を測る技術2015

    • Author(s)
      高橋英嗣
    • Organizer
      第54回日本生体医工学会大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2015-05-07
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] Gap cover glass (GCG)を用いた 2 次元 低酸素微小環境モデル2014

    • Author(s)
      吉田拓磨,矢原大裕,榎田祐也,高橋英嗣
    • Organizer
      第12回がんとハイポキシア研究会
    • Place of Presentation
      佐賀市
    • Year and Date
      2014-11-21 – 2014-11-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 酸素濃度勾配下での MDA-MB-231細胞の不均一分布2014

    • Author(s)
      矢原大裕,吉田拓磨,榎田祐也,高橋英嗣
    • Organizer
      第12回がんとハイポキシア研究会
    • Place of Presentation
      佐賀市
    • Year and Date
      2014-11-21 – 2014-11-22
    • Related Report
      2014 Research-status Report
  • [Presentation] 低酸素に対する細胞レベル・遺伝子レベルの適応2014

    • Author(s)
      高橋英嗣
    • Organizer
      第49回日本高気圧環境・ 潜水医学会学術総会
    • Place of Presentation
      鹿児島市
    • Year and Date
      2014-11-08
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 生体の低酸素適応メカニズム2014

    • Author(s)
      高橋英嗣
    • Organizer
      日大工学部次世代工学技術センターセミナー
    • Place of Presentation
      郡山市
    • Year and Date
      2014-10-31
    • Related Report
      2014 Research-status Report
    • Invited
  • [Book] 呼吸のトリビア32014

    • Author(s)
      高橋英嗣(分担執筆)
    • Total Pages
      165
    • Publisher
      中外医学社
    • Related Report
      2014 Research-status Report
  • [Remarks] 研究室の学生向けホームページ(日本語)

    • URL

      http://www.ee.saga-u.ac.jp/sensor/ATF_Biomed_J/Research.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究室の学生向けホームページ(英語)

    • URL

      http://www.ee.saga-u.ac.jp/sensor/ATF_Biomed/Research.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] Research

    • URL

      http://www.ee.saga-u.ac.jp/sensor/ATF_Biomed/Research.html

    • Related Report
      2015 Research-status Report
  • [Remarks] Research

    • URL

      http://www.ee.saga-u.ac.jp/sensor/ATF_Biomed_J/Research.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 先端融合工学専攻

    • URL

      http://www.fusion.saga-u.ac.jp/research/takahashi.html

    • Related Report
      2015 Research-status Report
  • [Remarks] ATF: BIOMED ENG TAKAHASHI LAB - RESEARCH

    • URL

      http://www.ee.saga-u.ac.jp/sensor/ATF_Biomed_J/Research.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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