| Project/Area Number |
26430117
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Saga University |
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Principal Investigator |
Takahashi Eiji 佐賀大学, 工学(系)研究科(研究院), 教授 (30206792)
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| Research Collaborator |
YAHARA Disuke 佐賀大学, 大学院工学系研究科, 大学院生
YOSHIDA Takuma 佐賀大学, 大学院工学系研究科, 大学院生
ENOKIDA Yuya 佐賀大学, 大学院工学系研究科, 大学院生
OKUBO Kaima 佐賀大学, 大学院工学系研究科, 大学院生
TSURUNO Yusuke 佐賀大学, 大学院工学系研究科, 大学院生
FUJIWARA Takahiro 佐賀大学, 大学院工学系研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | がん細胞 / 血行性遠隔転移 / 酸素 / pH / 細胞遊走 / 低酸素微小環境 / がん転移 / 酸素濃度勾配 / pH勾配 / イメージング / MDA-MB-231 / 腫瘍細胞遊走 / 転移 / 酸素測定 |
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| Outline of Final Research Achievements |
To elucidate the initial step of hematogenous metastasis of tumors, we hypothesized that migration of tumor cells might be directed by extracellular gradients of metabolic substrates/metabolites particularly by oxygen and/or H+. We first developed a novel technique to produce metabolic gradients in monolayers of cultured cell (MDA-MB-231 cells) in which cell migrations can be tracked for up to 24 hrs. We found significant directional migrations of the cell toward higher oxygen concentration or lower H+ concentration. These directional migrations were demonstrated in unphysiologically high oxygen concentrations (eg. 21%) while changes in extracellular H+ gradients in physiological ranges appeared to affect the directionality. From these results, we conclude that migrations of MDA-MB-231 cells in vitro seem to be directed by extracellular pH gradients.
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