Investigation of the global effect and the mechanism of cancer specific mature mRNA re-splicing
| Project/Area Number |
26430124
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Fujita Health University |
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Principal Investigator |
Kameyama Toshiki 藤田保健衛生大学, 総合医科学研究所, 助教 (60298544)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | がん / 異常スプライシング / RNA結合タンパク質 / siRNAライブラリー / siRNA / RBM4 / シスプラチン / mRNA再スプライシング / p53 / RNase R / 成熟mRNA再スプライシング / 次世代シークエンス |
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| Outline of Final Research Achievements |
We have discovered cancer-specific mRNA re-splicing that occurs on mature spliced mRNA and generates aberrant transcripts/proteins. The control of the re-splicing could be promoted by unknown activator upregulated and/or repressor downregulated in cancer cells.
Recent striking findings represent a breakthrough in the study of mRNA re-splicing. (1) TSG101 delta protein, generated via re-splicing of TSG101, enhances TSG101-stimulated cell proliferation and tumor growth. (2) mRNA re-splicing is repressed by the expression of TP53 often induced by cellular stress, (3) We have identified a repressor candidate of mRNA re-splicing by screening of siRNA library (156 kinds of RNA-binding proteins). Since the identified repressor is a known tumor suppressor, we postulate that global prevention of aberrant mRNA re-splicing, maintaining fidelity of splicing, is critical for the consequent tumor suppression. To prove this hypothesis is underway.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] ETV6-LPXN fusion transcript generated by t(11;12)(q12.1;p13) in a patient with relapsing acute myeloid leukemia with NUP98-HOXA9.2016
Author(s)
Abe A, Yamamoto Y, Iba S, Kanie T, Okamoto A, Tokuda M, Inaguma Y, Yanada M, Morishima S, Mizuta S, Akatsuka Y, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Genes, chromosomes & cancer
Volume: 55(3)
Issue: 3
Pages: 242-250
DOI
Related Report
Peer Reviewed
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[Journal Article] NUP214-RAC1 and RAC1-COL12A1 Fusion in Complex Variant Translocations Involving Chromosomes 6, 7 and 9 in an Acute Myeloid Leukemia Case with DEK-NUP214.2015
Author(s)
Abe A, Yamamoto Y, Iba S, Okamoto A, Tokuda M, Inaguma Y, Yanada M, Morishima S, Kanie T, Tsuzuki M, Akatsuka Y, Mizuta S, Okamoto M, Kameyama T, Mayeda A, Emi N.
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Journal Title
Cytogenetic and genome research
Volume: 146(4)
Issue: 4
Pages: 279-284
DOI
Related Report
Peer Reviewed
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[Journal Article] Fluvoxamine moderates reduced voluntary activity following chronic dexamethasone infusion in mice via recovery of BDNF signal cascades.2014
Author(s)
Terada K, *Izumo N, Suzuki B, Karube Y, Morikawa T, Ishibashi Y, Kameyama T, Chiba K, Sasaki N, Iwatae K, Matsuzaki H. and *Manabe T.
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Journal Title
Neurochem. Int.
Volume: 69
Pages: 9-13
Related Report
Peer Reviewed
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