| Project/Area Number |
26430125
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Kyoto Sangyo University |
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Principal Investigator |
ITANO Naoki 京都産業大学, 総合生命科学部, 教授 (40257712)
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| Research Collaborator |
IZUMIKAWA Tomomi 京都産業大学, 総合生命科学部, 研究助教
Chanmee Theerawut 京都産業大学, 総合生命科学部, 研究員
Ontong Pawared 京都産業大学, 大学院・工学研究科, 学生
MOCHIZUKI Nobutoshi 京都産業大学, 大学院・生命科学研究科, 学生
HIGASHIDE Miho 京都産業大学, 大学院・生命科学研究科, 学生
Chokchaitaweesuk Chatchadawarai 京都産業大学, 大学院・生命科学研究科, 学生
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | がん幹細胞 / ニッチ / ストレス耐性 / ヒアルロン酸 / 炎症 |
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| Outline of Final Research Achievements |
CSCs are thought to be responsible for tumor recurrence, due to their resistance to chemo- and radiotherapy. CSCs reside in a special microenvironmental niche that provides a favorable microenvironment for their self-renewal and maintenance. We have previously demonstrated that overproduction of hyaluronan (HA), a primary component of stem cell niche, promotes acquisition of CSC signatures. Here, we investigated the metabolic reprogramming in HA-overproducing cells by stable isotope-assisted tracing and mass spectrometry profiling. These integrated approaches disclosed an acceleration of metabolic flux in the hexosamine biosynthetic pathway (HBP). Overexpression or gene silencing of glutamine:fructose-6-phosphate amidotransferase 1, an HBP rate-limiting enzyme, suggests that HA production regulates drug resistance-related properties of CSCs via HBP.
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