Fundamental study of niche modification for reducing cancer stem cell properties
Project/Area Number |
26430125
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | Kyoto Sangyo University |
Principal Investigator |
ITANO Naoki 京都産業大学, 総合生命科学部, 教授 (40257712)
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Research Collaborator |
IZUMIKAWA Tomomi 京都産業大学, 総合生命科学部, 研究助教
Chanmee Theerawut 京都産業大学, 総合生命科学部, 研究員
Ontong Pawared 京都産業大学, 大学院・工学研究科, 学生
MOCHIZUKI Nobutoshi 京都産業大学, 大学院・生命科学研究科, 学生
HIGASHIDE Miho 京都産業大学, 大学院・生命科学研究科, 学生
Chokchaitaweesuk Chatchadawarai 京都産業大学, 大学院・生命科学研究科, 学生
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Project Period (FY) |
2014-04-01 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | がん幹細胞 / ニッチ / ストレス耐性 / ヒアルロン酸 / 炎症 |
Outline of Final Research Achievements |
CSCs are thought to be responsible for tumor recurrence, due to their resistance to chemo- and radiotherapy. CSCs reside in a special microenvironmental niche that provides a favorable microenvironment for their self-renewal and maintenance. We have previously demonstrated that overproduction of hyaluronan (HA), a primary component of stem cell niche, promotes acquisition of CSC signatures. Here, we investigated the metabolic reprogramming in HA-overproducing cells by stable isotope-assisted tracing and mass spectrometry profiling. These integrated approaches disclosed an acceleration of metabolic flux in the hexosamine biosynthetic pathway (HBP). Overexpression or gene silencing of glutamine:fructose-6-phosphate amidotransferase 1, an HBP rate-limiting enzyme, suggests that HA production regulates drug resistance-related properties of CSCs via HBP.
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Report
(5 results)
Research Products
(17 results)
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[Presentation] Hyaluronan production regulates metabolic and cancer stem-like properties of breast cancer cells via hexosamine biosynthetic pathway-coupled HIF-1 signaling2017
Author(s)
Itano, N., Chanmee, T., Ontong, P., Izumikawa, T., Higashide, M., Mochizuki, N., Chokchaitaweesuk, C., Khansai, M., Nakajima, K., Kakizaki, I., Kongtawelert, P., Taniguchi, N.
Organizer
International Society for Hyaluronan Sciences 11th International Conference (Hyaluronan 2017)
Related Report
Int'l Joint Research / Invited
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[Presentation] Hyaluronan production regulates metabolic and cancer stem-like properties of breast cancer cells via hexosamine biosynthetic pathway-coupled HIF-1 signaling2016
Author(s)
Tomomi Izumikawa, Theerawut Chanmee, Pawared Ontong, Miho Higashide, Nobutoshi Mochizuki, Chatchadawalai Chokchaitaweesuk, Manatsanan Khansai, Kazuki Nakajima, Ikuko Kakizaki, Prachya Kongtawelert, Naoyuki Taniguchi, Naoki Itano
Organizer
第89回日本生化学会大会
Place of Presentation
仙台国際センター(宮城県・仙台市)
Year and Date
2016-09-27
Related Report
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[Presentation] Excessive hyaluronan production promotes acquisition of cancer stem signatures2015
Author(s)
Itano, N. Chanmee, T., Ontong, P., Mochizuki, N., Kongtawelert, P., Konno, K.
Organizer
International Society for Hyaluronan Sciences 10th International Conference
Place of Presentation
Florence, Italy
Year and Date
2015-07-11
Related Report
Int'l Joint Research / Invited
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