The elucidation of function of long noncoding RNAs induced by oncogenic signal and the exploration of their inhibitors.
Project/Area Number |
26430127
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | Kindai University |
Principal Investigator |
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | ガン化シグナル / 長鎖ノンコーディングRNA / 細胞周期 / 癌化シグナル / lncRNA / long noncoding RNA / Cell cycle / Apoptosis / Oncogene |
Outline of Final Research Achievements |
In this study, we revealed that a long noncoding RNA, PANDA, induced by oncogenic signal stabilizes p53 tumor suppressor protein in response to DNA damage. Quantitative RT-PCR assay showed that PANDA was highly expressed in U2OS human osteosarcoma cells. Silencing PANDA caused arrest at the G1 phase of the cell cycle, leading to inhibition of cell proliferation. We revealed that PANDA promotes G1-S transition by repressing p18 transcription, and thus promotes U2OS cell proliferation. These results indicate that PANDA may be a good molecular target for cancer treatment drug.
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Report
(4 results)
Research Products
(61 results)
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[Journal Article] Silencing of bcr/abl Chimeric Gene in Human Chronic Myelogenous Leukemia Cell Line K562 by siRNA-Nuclear Export Signal Peptide Conjugates.2017
Author(s)
Shinkai S, Kashihara S, Minematsu G, Fujii H, Naemura M, Kotake Y, Morita Y, Ohnuki K, Fokina AA, Stetsenko DA, Filichev VV, Fujii M.
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Journal Title
Nucleic Acid Therapeutics
Volume: 印刷中
Issue: 3
Pages: 168-175
DOI
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Peer Reviewed / Int'l Joint Research
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[Journal Article] YB-1 promotes transcription of cyclin D1 in human non-small-cell lung cancers2014
Author(s)
Harada, M., Kotake, Y., Ohhata, T., Kitagawa, K., Niida, H., Matsuura, S., Funai, K., Sugimura, H., Suda, T. and Kitagawa, M.
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Journal Title
Genes Cells
Volume: 18
Issue: 6
Pages: 999-1006
DOI
Related Report
Peer Reviewed
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[Presentation] 化学修飾siRNAのRNA干渉効果への影響2016
Author(s)
新貝恭広, 苗村円佳, 神武洋二郎, 山吉麻子, 有吉順平, Alesya A. Fokina, Dmitry A. Stetsenko, Vyacheslav V. Filichev, 藤井政幸.
Organizer
日本核酸医薬学会第二回年会
Place of Presentation
東京理科大学葛飾キャンパス(葛飾区)
Year and Date
2016-11-15
Related Report
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[Presentation] LONG NONCODING RNA ANRIL PROMOTES PROLIFERATION OF CANCER CELLS2015
Author(s)
Madoka Naemura, Chihiro Murasaki, Yasutoshi Inoue, Haruna Okamoto, and Yojiro Kotake
Organizer
Joint International Symposium on「Regional Revitalization and Innovation for Social Contribution」 and and「e-ASIA Functional Materials and Biomass Utilization 2015」
Place of Presentation
福岡県立大学(田川市)
Year and Date
2015-11-01
Related Report
Int'l Joint Research
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[Presentation] 化学修飾核酸の新規合成法2015
Author(s)
江見友裕, 髙松辰臣, 岡本大幹, 苗村円佳, 神武洋二郎, 藤井政幸
Organizer
第52回化学関連支部合同九州大会
Place of Presentation
北九州国際会議場(北九州市)
Year and Date
2015-06-27
Related Report
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[Presentation] カチオン性siRNAの遺伝子サイレンシング効果2014
Author(s)
高科あゆみ, 萱野光, 江見友裕, 苗村円佳, 神武洋二郎, 有吉純平, 山吉麻子, 村上章, 藤井政幸
Organizer
アンチセンス・遺伝子・デリバリーシンポジウム
Place of Presentation
東京医科歯科大学(東京都文京区)
Year and Date
2014-09-08
Related Report
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