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Lung carcinogenesis by genetic reconstitution using organoids derived from normal lung in mice

Research Project

Project/Area Number 26430134
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor biology
Research InstitutionNational Cancer Center Japan

Principal Investigator

Ochiai Masako  国立研究開発法人国立がん研究センター, 研究所, 主任研究員 (90150200)

Co-Investigator(Kenkyū-buntansha) 筆宝 義隆  国立研究開発法人国立がん研究センター, 研究所, 客員研究員 (30359632)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords正常肺3D培養 / in vitro肺発がんモデル
Outline of Final Research Achievements

We aimed to establish the 3D-cultured cells (organoids) from normal lung tissues in mice and to construct an in vitro model of lung carcinogenesis using genetic reconstitution, which is transduction by shRNAs of tumor suppressor genes (TSG) using lentivirus. From the examination of culture conditions, it was suitable for lung organoids culture to the addition of inhibitor X related to a signal transduction and growth factor Y with proliferative effect for alveolar cells type II. These organoids with transduction of TSG shRNAs were injected to nude mice subcutaneously and developed tumors.
In application to in vitro evaluation system of lung carcinogenesis by chemicals, lung carcinogen C as positive control is possible dose setting in the combination to chemical exposure. After multiple exposures, it was formed subcutaneous tumor in nude mice and it was shown to dysplastic features. It is indicated that dysplastic changes can be an marker for carcinogenic potentials of chemicals.

Report

(5 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Products Report
  • Research Products

    (10 results)

All 2019 2016 2015 2014 Other

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (7 results) Remarks (2 results)

  • [Journal Article] Kras-driven heterotopic tumor development from hepatobiliary organoids2019

    • Author(s)
      Ochiai Masako、Yoshihara Yasunori、Maru Yoshiaki、Matsuura Tetsuya、Izumiya Masashi、Imai Toshio、Hippo Yoshitaka
    • Journal Title

      Carcinogenesis

      Volume: 印刷中 Pages: 1142-1152

    • DOI

      10.1093/carcin/bgz024

    • Related Report
      Products Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] マウス正常大腸上皮細胞の3次元培養によるin vitro発がんモデルの開発2016

    • Author(s)
      落合雅子、松浦哲也、中釜斉、筆宝義隆、今井俊夫
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      神奈川県・横浜市
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス正常大腸上皮細胞の3次元培養によるin vitro発がんモデルの開発―化学発がん・予防研究への応用に向けて2016

    • Author(s)
      落合雅子、松浦哲也、筆宝義隆、今井俊夫
    • Organizer
      がん予防学術大会2016名古屋
    • Place of Presentation
      愛知県・名古屋市
    • Year and Date
      2016-07-01
    • Related Report
      2016 Annual Research Report
  • [Presentation] マウス正常上皮細胞の3次元培養法を用いるin vitro発がんモデルの開発2015

    • Author(s)
      落合雅子、中釜 斉
    • Organizer
      日本環境変異原学会第44回大会
    • Place of Presentation
      福岡県福岡市
    • Year and Date
      2015-11-27
    • Related Report
      2015 Research-status Report
  • [Presentation] マウス正常腸管上皮の3次元培養法を用いる発がん再構成系に対するPhIPの修飾作用2015

    • Author(s)
      落合雅子、松浦哲也、中釜 斉、筆宝義隆、今井俊夫
    • Organizer
      第30回発癌病理研究会
    • Place of Presentation
      香川県小豆郡土庄町
    • Year and Date
      2015-08-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 正常上皮細胞の3次元培養によるin vitro発がんモデルの開発 -化学発がん・予防研究への応用に向けて2015

    • Author(s)
      落合雅子、松浦哲也、中釜 斉、筆宝義隆、今井俊夫
    • Organizer
      がん予防学術大会2015さいたま
    • Place of Presentation
      埼玉県さいたま市
    • Year and Date
      2015-06-05
    • Related Report
      2015 Research-status Report
  • [Presentation] マウス肝臓初代培養細胞を用いた新規肝内胆管がんモデルの確立2014

    • Author(s)
      落合雅子,折橋 郁,松浦哲也,今井俊夫,中釜 斉,筆宝義隆
    • Organizer
      第73回日本癌学会学術総会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] マウス肝臓初代培養細胞を用いた新規肝内胆管がんモデルの確立2014

    • Author(s)
      落合雅子,折橋 郁,松浦哲也,今井俊夫,中釜 斉,筆宝義隆
    • Organizer
      第29回発癌病理研究会
    • Place of Presentation
      福島県いわき市
    • Year and Date
      2014-09-01 – 2014-09-03
    • Related Report
      2014 Research-status Report
  • [Remarks] 正常組織の3次元培養法を応用したモデルの開発(動物実験部門 )

    • URL

      https://www.nccri.ncc.go.jp/s029/010/010/20151216104229.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 正常組織の3次元培養法を応用したモデルの開発(動物実験部門 )

    • URL

      http://www.nccri.ncc.go.jp/s029/010/010/20151216104229.html

    • Related Report
      2015 Research-status Report

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Published: 2014-04-04   Modified: 2021-07-06  

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