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Identification of Novel Treatment Targets and Biomarkers for Small-Cell Lung Cancer by Circulating Tumor Cell Analysis

Research Project

Project/Area Number 26430142
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Tumor diagnostics
Research InstitutionKanazawa University

Principal Investigator

Kimura Hideharu  金沢大学, 医学系, 助教 (40444202)

Co-Investigator(Kenkyū-buntansha) 笠原 寿郎  金沢大学, 医学系, 准教授 (30272967)
曽根 崇  金沢大学, 医薬保健学総合研究科, 特任准教授 (30420334)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords肺癌 / 血中DNA / 循環腫瘍細胞 / 小細胞肺癌
Outline of Final Research Achievements

[Gene expression analysis using circulating tumor cells (CTCs)] We extracted RNA from the peripheral blood mononuclear cell fraction of 12 small-cell lung cancer patients and analyzed it for CTC-derived gene expression by the reverse transcriptase-polymerase chain reaction (RT-PCR). We investigated neuroendocrine-related, epithelial-mesenchymal-transition-related, and angiogenesis-related gene expression and were able to identify epithelial-mesenchymal transition-related gene expression. The results suggested that it is possible to identify CTC-related gene expression by this method.
[Gene mutation analysis using blood DNA] We analyzed tumor tissue derived DNA and blood DNA from 4 autopsy cases of advanced lung cancer by using a next generation sequencer. The same gene mutations as in the blood DNA were present in multiple lesions, and allele frequency in the tumor tissue was significantly higher.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (13 results)

All 2017 2016 2015 2014 2013

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 5 results,  Acknowledgement Compliant: 2 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Imatinib ameliorates bronchiolitis obliterans via inhibition of fibrocyte migration and differentiation.2017

    • Author(s)
      1.4.Watanabe S, Kasahara K, Waseda Y, Takato H, Nishikawa S, Yoneda T, Hara J, Sone T, Abo M, Kimura H, Nakao S.
    • Journal Title

      J Heart Lung Transplant.

      Volume: 36 Issue: 2 Pages: 138-147

    • DOI

      10.1016/j.healun.2016.06.001

    • NAID

      120005983030

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 1.1.A Rapid and Sensitive Method for Detection of the T790M Mutation of EGFR in Plasma DNA.2016

    • Author(s)
      Kimura H, Nishikawa S, Koba H, Yoneda T, Sone T, Kasahara K.
    • Journal Title

      Adv Exp Med Biol.

      Volume: 924 Pages: 171-174

    • DOI

      10.1007/978-3-319-42044-8_31

    • ISBN
      9783319420424, 9783319420448
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 1.2.Impact of Tight Junction Protein ZO-1 and TWIST Expression on Postoperative Survival of Patients with Hepatocellular Carcinoma.2016

    • Author(s)
      Nagai T, Arao T, Nishio K, Matsumoto K, Hagiwara S, Sakurai T, Minami Y, Ida H, Ueshima K, Nishida N, Sakai K, Saijo N, Kudo K, Kaneda H, Tamura D, Aomatsu K, Kimura H, Fujita Y, Haji S, Kudo M.
    • Journal Title

      Dig Dis.

      Volume: 34 Issue: 6 Pages: 702-707

    • DOI

      10.1159/000448860

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Postoperative Recurrence of Invasive Thymoma with Cold Agglutinin Disease and Autoimmune Hemolytic Anemia2016

    • Author(s)
      1.3.Yoneda T, Koba H, Tanimura K, Ogawa N, Watanabe S, Hara J, Abo M, Sone T, Kimura H, Kasahara K.
    • Journal Title

      Internal Medicine

      Volume: 55 Issue: 18 Pages: 2685-2689

    • DOI

      10.2169/internalmedicine.55.6654

    • NAID

      130005414857

    • ISSN
      0918-2918, 1349-7235
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Severe pneumonitis after nivolumab treatment in a patient with melanoma.2016

    • Author(s)
      1.5.Watanabe S, Kimura H, Takato H, Waseda Y, Hara J, Sone T, Abo M, Maeda S, Matsushita T,Kasahara K.
    • Journal Title

      Allergol Int.

      Volume: 65 Issue: 4 Pages: 487-489

    • DOI

      10.1016/j.alit.2016.04.009

    • NAID

      130005431308

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Imatinib for bronchiolitis obliterans after allogeneic hematopoietic stem cell transplantation.2015

    • Author(s)
      1.1.Watanabe S, Waseda Y, Kimura H, Takato H, Ohata K, Kondo Y, Kasahara K, Nakao S.
    • Journal Title

      Bone Marrow Transplant.

      Volume: 50 Issue: 9 Pages: 1250-1252

    • DOI

      10.1038/bmt.2015.120

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Analytical performance of the cobas EGFR2014

    • Author(s)
      Kimura H, Nagao T, 他6名
    • Journal Title

      Mutation assay for Japanese non-small-cell lung cancer

      Volume: 83 Issue: 3 Pages: 329-33

    • DOI

      10.1016/j.lungcan.2013.12.012

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Biomarkers of reactive resistance and early disease progression during chemotherapy plus bevacizumab treatment for colorectal carcinoma.2014

    • Author(s)
      Hayashi H, Arao T, Matsumoto K, Kimura H, Togashi Y, Hirashima Y, Horita Y, Iwasa S, Okita NT, Honma Y, Takashima A, Kato K, Hamaguchi T, Shimada Y, Nakagawa K, Nishio K, Yamada Y.
    • Journal Title

      Oncotarget

      Volume: 5 Pages: 2588-2595

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Frequent amplification of ORAOV1 gene in esophageal squamous cell cancer promotes an aggressive phenotype via proline metabolism and ROS production.2014

    • Author(s)
      Togashi Y, Arao T, Kato H, Matsumoto K, Terashima M, Hayashi H, de Velasco MA, Fujita Y, Kimura H, Yasuda T, Shiozaki H, Nishio K.
    • Journal Title

      Oncotarget

      Volume: 5 Pages: 2962-2973

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] The OCT4 pseudogene POU5F1B is amplified and promotes an aggressive phenotype in gastric cancer.2013

    • Author(s)
      Hayashi, H., Arao, T., Togashi, Y., Kato, H., Velasco, MA., Kimura, H., Matsumoto, K., Tanaka, K., Okamoto, I., Ito, A., Yamada, Y., Nakagawa, K., Nishio, K.
    • Journal Title

      Oncogene

      Volume: E-pub ahead or print Issue: 2 Pages: 199-208

    • DOI

      10.1038/onc.2013.547

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Next-generation sequencing analysis identifies genomic alterations in pathological morphologies  a case report of pulmonary carcinosarcoma harboring EGFR mutations.2016

    • Author(s)
      Hayato Koba, Hideharu Kimura, Shingo Nishikawa, Yuichi Tambo, Takashi Sone, Atsushi Tajima,  Kazuo Kasahara
    • Organizer
      ESMO ASIA 2016
    • Place of Presentation
      シンガポール
    • Year and Date
      2016-12-16
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Non-invasive analysis for T790M mutations of EGFR using a selective amplification method2015

    • Author(s)
      Shingo Nishikawa, Hideharu Kimura, Hayato Koba, Taro Yoneda, Takashi Sone, Chris Booth, Andrew Webb, Kazuo Kasahara
    • Organizer
      AACR Annual Meeting 2015
    • Place of Presentation
      Philadelphia
    • Year and Date
      2015-04-18
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] Noninvasive analysis of acquired resistance to EGFR-TKI2014

    • Author(s)
      Hideharu Kimura, Makoto Nishio, Tsutomu Daito, Kazuto Nishio.
    • Organizer
      AACR Annual Meeting 2014
    • Place of Presentation
      San Diego
    • Year and Date
      2014-04-05 – 2014-04-09
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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