Identification of Novel Treatment Targets and Biomarkers for Small-Cell Lung Cancer by Circulating Tumor Cell Analysis
Project/Area Number |
26430142
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor diagnostics
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Research Institution | Kanazawa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
笠原 寿郎 金沢大学, 医学系, 准教授 (30272967)
曽根 崇 金沢大学, 医薬保健学総合研究科, 特任准教授 (30420334)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | 肺癌 / 血中DNA / 循環腫瘍細胞 / 小細胞肺癌 |
Outline of Final Research Achievements |
[Gene expression analysis using circulating tumor cells (CTCs)] We extracted RNA from the peripheral blood mononuclear cell fraction of 12 small-cell lung cancer patients and analyzed it for CTC-derived gene expression by the reverse transcriptase-polymerase chain reaction (RT-PCR). We investigated neuroendocrine-related, epithelial-mesenchymal-transition-related, and angiogenesis-related gene expression and were able to identify epithelial-mesenchymal transition-related gene expression. The results suggested that it is possible to identify CTC-related gene expression by this method. [Gene mutation analysis using blood DNA] We analyzed tumor tissue derived DNA and blood DNA from 4 autopsy cases of advanced lung cancer by using a next generation sequencer. The same gene mutations as in the blood DNA were present in multiple lesions, and allele frequency in the tumor tissue was significantly higher.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Imatinib ameliorates bronchiolitis obliterans via inhibition of fibrocyte migration and differentiation.2017
Author(s)
1.4.Watanabe S, Kasahara K, Waseda Y, Takato H, Nishikawa S, Yoneda T, Hara J, Sone T, Abo M, Kimura H, Nakao S.
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Journal Title
J Heart Lung Transplant.
Volume: 36
Issue: 2
Pages: 138-147
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] 1.2.Impact of Tight Junction Protein ZO-1 and TWIST Expression on Postoperative Survival of Patients with Hepatocellular Carcinoma.2016
Author(s)
Nagai T, Arao T, Nishio K, Matsumoto K, Hagiwara S, Sakurai T, Minami Y, Ida H, Ueshima K, Nishida N, Sakai K, Saijo N, Kudo K, Kaneda H, Tamura D, Aomatsu K, Kimura H, Fujita Y, Haji S, Kudo M.
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Journal Title
Dig Dis.
Volume: 34
Issue: 6
Pages: 702-707
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Postoperative Recurrence of Invasive Thymoma with Cold Agglutinin Disease and Autoimmune Hemolytic Anemia2016
Author(s)
1.3.Yoneda T, Koba H, Tanimura K, Ogawa N, Watanabe S, Hara J, Abo M, Sone T, Kimura H, Kasahara K.
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Journal Title
Internal Medicine
Volume: 55
Issue: 18
Pages: 2685-2689
DOI
NAID
ISSN
0918-2918, 1349-7235
Related Report
Peer Reviewed
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[Journal Article] Biomarkers of reactive resistance and early disease progression during chemotherapy plus bevacizumab treatment for colorectal carcinoma.2014
Author(s)
Hayashi H, Arao T, Matsumoto K, Kimura H, Togashi Y, Hirashima Y, Horita Y, Iwasa S, Okita NT, Honma Y, Takashima A, Kato K, Hamaguchi T, Shimada Y, Nakagawa K, Nishio K, Yamada Y.
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Journal Title
Oncotarget
Volume: 5
Pages: 2588-2595
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Frequent amplification of ORAOV1 gene in esophageal squamous cell cancer promotes an aggressive phenotype via proline metabolism and ROS production.2014
Author(s)
Togashi Y, Arao T, Kato H, Matsumoto K, Terashima M, Hayashi H, de Velasco MA, Fujita Y, Kimura H, Yasuda T, Shiozaki H, Nishio K.
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Journal Title
Oncotarget
Volume: 5
Pages: 2962-2973
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The OCT4 pseudogene POU5F1B is amplified and promotes an aggressive phenotype in gastric cancer.2013
Author(s)
Hayashi, H., Arao, T., Togashi, Y., Kato, H., Velasco, MA., Kimura, H., Matsumoto, K., Tanaka, K., Okamoto, I., Ito, A., Yamada, Y., Nakagawa, K., Nishio, K.
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Journal Title
Oncogene
Volume: E-pub ahead or print
Issue: 2
Pages: 199-208
DOI
Related Report
Peer Reviewed / Open Access
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