Development of an early diagnostic system for metastasis using the mass spectrometer

• Project/Area Number: 26430143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Kobe University
• Principal Investigator: Hatano Naoya 神戸大学, 医学研究科, 特命助教 (10332280)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: バイオマーカー / メタボローム / がん

Outline of Final Research Achievements

Cancer is leading cause of death in Japan. It is known that the metastasis has occurred in most cases of death. There is no method to find the metastasis at an early stage. Therefore, a low-cost, easy-to-use, and high-sensitive cancer metastasis screening method is required.
Initiation metastasis required invasion, which is enabled by epithelial-mesenchymal transition(EMT). Here, we detected the biomarker candidates for metastasis using EMT-induced breast cancer cells.

Research Products

• [Journal Article] Glucose-regulated protein 78 (GRP78) binds directly to PIP3 phosphatase SKIP and determines its localization. (2016)
  • Author(s): Ijuin T, Hatano N, Takenawa T.
  • Journal Title: Genes to Cells Volume: 21(5) Issue: 5 Pages: 457-465
  • DOI: 10.1111/gtc.12353
  • Peer Reviewed
• [Journal Article] Identification of putative biomarkers for prediabetes by metabolome analysis of rat models of type 2 diabetes (2015)
  • Author(s): Yokoi N, Beppu M, Yoshida E, Hoshikawa R, Hidaka S, Matsubara T, Shinohara M, Irino Y, Hatano N, Seino S
  • Journal Title: Metabolomics Volume: 印刷中 Issue: 5 Pages: 1277-1286
  • DOI: 10.1007/s11306-015-0784-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Regulation of insulin signaling in skeletal muscle by PIP3 phosphatase, SKIP, and endoplasmic reticulum molecular chaperone glucose-regulated protein 78. (2015)
  • Author(s): Ijuin T, Hatano N, Hosooka T, Takenawa T.
  • Journal Title: Biochimica et Biophysica Acta Volume: 1853(12) Issue: 12 Pages: 3192-3201
  • DOI: 10.1016/j.bbamcr.2015.09.009
  • Peer Reviewed
• [Journal Article] Glutamate acts as a key signal linking glucose metabolism to incretin/cAMP action to amplify insulin secretion (2014)
  • Author(s): Gheni G, Ogura M, Iwasaki M, Yokoi N, Minami K, Nakayama Y, Harada K, Hastoy B, Wu X, Takahashi H, Kimura K, Matsubara T, Hoshikawa R, Hatano N, Sugawara K, Shibasaki T, Inagaki N, Bamba T, Mizoguchi A, Fukusaki E, Rorsman P, Seino S
  • Journal Title: Cell Rep Volume: 9 Issue: 2 Pages: 661-673
  • DOI: 10.1016/j.celrep.2014.09.030
  • Peer Reviewed / Open Access / Acknowledgement Compliant